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Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion.
Chin Med J (Engl). 2014; 127(19):3378-82.CM

Abstract

BACKGROUND

Preeclampsia (PE) is associated with abnormal fatty acid beta-oxidation (FAO), especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is unclear. The aim of this study was to explore the influence of various lengths fatty acids oxidation on invasiveness of trophoblasts.

METHODS

Primary human trophoblast cells and HTR8/SVneo cells were treated with fatty acids of various lengths. Morphological changes, lipid deposition and ultrastructure changes of trophoblast cells were detected. Cells invasiveness was determined by transwell insert. CPT1, CPT2 and long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) protein expression were analyzed. The correlation between intracellular lipid droplets deposition and cells invasiveness was evaluated.

RESULTS

Cells treated with long-chain fatty acids showed significant increased lipid droplets deposition, severe mitochondrial damage, decreased CPT2 and LCHAD protein expression (P < 0.05) but no significant difference in CPT1 protein expression (P > 0.05). Invasiveness of the trophoblast cells of the LC-FFA group significantly decreased (P < 0.05). Intracellular lipid droplets deposition was negatively correlated with invasivenss (R = -0.745, P < 0.05).

CONCLUSION

Trophoblast cells after stimulation with long chain fatty acids exist fatty acid oxidation disorders, and reduce the ability of trophoblastic invasion.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China. Email: zi_yang@email.com.Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25269899

Citation

Yu, Huan, et al. "Correlation Between the Different Chain Lengths of Free Fatty Acid Oxidation and Ability of Trophoblastic Invasion." Chinese Medical Journal, vol. 127, no. 19, 2014, pp. 3378-82.
Yu H, Yang Z, Ding X, et al. Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion. Chin Med J (Engl). 2014;127(19):3378-82.
Yu, H., Yang, Z., Ding, X., Wang, Y., & Han, Y. (2014). Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion. Chinese Medical Journal, 127(19), 3378-82.
Yu H, et al. Correlation Between the Different Chain Lengths of Free Fatty Acid Oxidation and Ability of Trophoblastic Invasion. Chin Med J (Engl). 2014;127(19):3378-82. PubMed PMID: 25269899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation between the different chain lengths of free fatty acid oxidation and ability of trophoblastic invasion. AU - Yu,Huan, AU - Yang,Zi, AU - Ding,Xiaoyan, AU - Wang,Yanling, AU - Han,Yiwei, PY - 2014/10/2/entrez PY - 2014/10/2/pubmed PY - 2015/4/10/medline SP - 3378 EP - 82 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 127 IS - 19 N2 - BACKGROUND: Preeclampsia (PE) is associated with abnormal fatty acid beta-oxidation (FAO), especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is unclear. The aim of this study was to explore the influence of various lengths fatty acids oxidation on invasiveness of trophoblasts. METHODS: Primary human trophoblast cells and HTR8/SVneo cells were treated with fatty acids of various lengths. Morphological changes, lipid deposition and ultrastructure changes of trophoblast cells were detected. Cells invasiveness was determined by transwell insert. CPT1, CPT2 and long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) protein expression were analyzed. The correlation between intracellular lipid droplets deposition and cells invasiveness was evaluated. RESULTS: Cells treated with long-chain fatty acids showed significant increased lipid droplets deposition, severe mitochondrial damage, decreased CPT2 and LCHAD protein expression (P < 0.05) but no significant difference in CPT1 protein expression (P > 0.05). Invasiveness of the trophoblast cells of the LC-FFA group significantly decreased (P < 0.05). Intracellular lipid droplets deposition was negatively correlated with invasivenss (R = -0.745, P < 0.05). CONCLUSION: Trophoblast cells after stimulation with long chain fatty acids exist fatty acid oxidation disorders, and reduce the ability of trophoblastic invasion. SN - 2542-5641 UR - https://www.unboundmedicine.com/medline/citation/25269899/Correlation_between_the_different_chain_lengths_of_free_fatty_acid_oxidation_and_ability_of_trophoblastic_invasion_ L2 - https://Insights.ovid.com/pubmed?pmid=25269899 DB - PRIME DP - Unbound Medicine ER -