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Profile of skin barrier proteins (filaggrin, claudins 1 and 4) and Th1/Th2/Th17 cytokines in adults with atopic dermatitis.
J Eur Acad Dermatol Venereol. 2015 Jun; 29(6):1091-5.JE

Abstract

BACKGROUND

Atopic dermatitis (AD) in adults and profile of skin barrier proteins and inflammatory cytokines.

OBJECTIVE

Evaluation of the expression of skin barrier proteins such as filaggrin, claudins 1 and 4 and of circulating inflammatory cytokines (Th1/Th2/Th17) in adults with AD.

METHODS

Thirty-three adult patients with AD diagnosed according to the Hanifin & Rajkacriteria, and 25 healthy controls were enrolled in the study. AD severity was measured by Eczema Area and Severity Index (EASI). Laboratory assays included immunohistochemistry analysis of skin barrier proteins, such as filaggrin, claudins 1 and 4 and interleukin-17 (IL-17) from skin samples and determination of circulating cytokine levels (IL-2, 4, 5, 6, 10, 17A, TNF and IFN-γ) by flow cytometry (Cytometric Bead Array).

RESULTS

We observed a reduced expression of filaggrin and claudin 1 in lesional skin of AD patients, when compared to controls. There was an inverse correlation of filaggrin expression and disease severity. In addition, IL-17 expression was enhanced in AD patients. Similarly, higher levels of inflammatory cytokines (IL-2, 5, 6, 10, 17A and IFN-γ) were found in AD patients.

CONCLUSION

Our data reinforce the role of an altered skin barrier in the pathogenesis of AD. Our results show not only reduced expression of filaggrin and claudin 1 in lesional atopic skin but also inverse correlation of filaggrin expression and disease severity. Moreover, elevation of in situ IL-17 and of circulating interleukin levels in AD emphasize the systemic, inflammatory profile of this defective skin barrier dermatosis.

Authors+Show Affiliations

Department of Dermatology, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.Department of Dermatology, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.Department of Dermatology, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.Department of Dermatology, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.Department of Dermatology, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.Laboratory of Medical Investigation in Dermatology and Immunodeficiency, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.Department of Dermatology, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.Department of Dermatology, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.Laboratory of Medical Investigation in Dermatology and Immunodeficiency, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.Laboratory of Medical Investigation in Dermatology and Immunodeficiency, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.Department of Dermatology, University of Sao Paulo School of Medicine, Sao Paulo, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25271795

Citation

Batista, D I S., et al. "Profile of Skin Barrier Proteins (filaggrin, Claudins 1 and 4) and Th1/Th2/Th17 Cytokines in Adults With Atopic Dermatitis." Journal of the European Academy of Dermatology and Venereology : JEADV, vol. 29, no. 6, 2015, pp. 1091-5.
Batista DI, Perez L, Orfali RL, et al. Profile of skin barrier proteins (filaggrin, claudins 1 and 4) and Th1/Th2/Th17 cytokines in adults with atopic dermatitis. J Eur Acad Dermatol Venereol. 2015;29(6):1091-5.
Batista, D. I., Perez, L., Orfali, R. L., Zaniboni, M. C., Samorano, L. P., Pereira, N. V., Sotto, M. N., Ishizaki, A. S., Oliveira, L. M., Sato, M. N., & Aoki, V. (2015). Profile of skin barrier proteins (filaggrin, claudins 1 and 4) and Th1/Th2/Th17 cytokines in adults with atopic dermatitis. Journal of the European Academy of Dermatology and Venereology : JEADV, 29(6), 1091-5. https://doi.org/10.1111/jdv.12753
Batista DI, et al. Profile of Skin Barrier Proteins (filaggrin, Claudins 1 and 4) and Th1/Th2/Th17 Cytokines in Adults With Atopic Dermatitis. J Eur Acad Dermatol Venereol. 2015;29(6):1091-5. PubMed PMID: 25271795.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Profile of skin barrier proteins (filaggrin, claudins 1 and 4) and Th1/Th2/Th17 cytokines in adults with atopic dermatitis. AU - Batista,D I S, AU - Perez,L, AU - Orfali,R L, AU - Zaniboni,M C, AU - Samorano,L P, AU - Pereira,N V, AU - Sotto,M N, AU - Ishizaki,A S, AU - Oliveira,L M S, AU - Sato,M N, AU - Aoki,V, Y1 - 2014/10/01/ PY - 2014/05/08/received PY - 2014/08/13/accepted PY - 2014/10/2/entrez PY - 2014/10/2/pubmed PY - 2016/3/24/medline SP - 1091 EP - 5 JF - Journal of the European Academy of Dermatology and Venereology : JEADV JO - J Eur Acad Dermatol Venereol VL - 29 IS - 6 N2 - BACKGROUND: Atopic dermatitis (AD) in adults and profile of skin barrier proteins and inflammatory cytokines. OBJECTIVE: Evaluation of the expression of skin barrier proteins such as filaggrin, claudins 1 and 4 and of circulating inflammatory cytokines (Th1/Th2/Th17) in adults with AD. METHODS: Thirty-three adult patients with AD diagnosed according to the Hanifin & Rajkacriteria, and 25 healthy controls were enrolled in the study. AD severity was measured by Eczema Area and Severity Index (EASI). Laboratory assays included immunohistochemistry analysis of skin barrier proteins, such as filaggrin, claudins 1 and 4 and interleukin-17 (IL-17) from skin samples and determination of circulating cytokine levels (IL-2, 4, 5, 6, 10, 17A, TNF and IFN-γ) by flow cytometry (Cytometric Bead Array). RESULTS: We observed a reduced expression of filaggrin and claudin 1 in lesional skin of AD patients, when compared to controls. There was an inverse correlation of filaggrin expression and disease severity. In addition, IL-17 expression was enhanced in AD patients. Similarly, higher levels of inflammatory cytokines (IL-2, 5, 6, 10, 17A and IFN-γ) were found in AD patients. CONCLUSION: Our data reinforce the role of an altered skin barrier in the pathogenesis of AD. Our results show not only reduced expression of filaggrin and claudin 1 in lesional atopic skin but also inverse correlation of filaggrin expression and disease severity. Moreover, elevation of in situ IL-17 and of circulating interleukin levels in AD emphasize the systemic, inflammatory profile of this defective skin barrier dermatosis. SN - 1468-3083 UR - https://www.unboundmedicine.com/medline/citation/25271795/Profile_of_skin_barrier_proteins__filaggrin_claudins_1_and_4__and_Th1/Th2/Th17_cytokines_in_adults_with_atopic_dermatitis_ L2 - https://doi.org/10.1111/jdv.12753 DB - PRIME DP - Unbound Medicine ER -