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Improved ethanol production from xylose in the presence of acetic acid by the overexpression of the HAA1 gene in Saccharomyces cerevisiae.
J Biosci Bioeng. 2015 Mar; 119(3):297-302.JB

Abstract

The hydrolysis of lignocellulosic biomass liberates sugars, primarily glucose and xylose, which are subsequently converted to ethanol by microbial fermentation. The rapid and efficient fermentation of xylose by recombinant Saccharomyces cerevisiae strains is limited by weak acids generated during biomass pretreatment processes. In particular, acetic acid negatively affects cell growth, xylose fermentation rate, and ethanol production. The ability of S. cerevisiae to efficiently utilize xylose in the presence of acetic acid is an essential requirement for the cost-effective production of ethanol from lignocellulosic hydrolysates. Here, an acetic acid-responsive transcriptional activator, HAA1, was overexpressed in a recombinant xylose-fermenting S. cerevisiae strain to yield BY4741X/HAA1. This strain exhibited improved cell growth and ethanol production from xylose under aerobic and oxygen limited conditions, respectively, in the presence of acetic acid. The HAA1p regulon enhanced transcript levels in BY4741X/HAA1. The disruption of PHO13, a p-nitrophenylphosphatase gene, in BY4741X/HAA1 led to further improvement in both yeast growth and the ability to ferment xylose, indicating that HAA1 overexpression and PHO13 deletion act by different mechanisms to enhance ethanol production.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Sakihama Y
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
Hasunuma T
Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
Kondo A
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. Electronic address: akondo@kobe-u.ac.jp.

MeSH

4-NitrophenylphosphataseAcetic AcidAerobiosisCulture MediaEthanolFermentationGene ExpressionSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsXylose

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25282639

Citation

Sakihama, Yuri, et al. "Improved Ethanol Production From Xylose in the Presence of Acetic Acid By the Overexpression of the HAA1 Gene in Saccharomyces Cerevisiae." Journal of Bioscience and Bioengineering, vol. 119, no. 3, 2015, pp. 297-302.
Sakihama Y, Hasunuma T, Kondo A. Improved ethanol production from xylose in the presence of acetic acid by the overexpression of the HAA1 gene in Saccharomyces cerevisiae. J Biosci Bioeng. 2015;119(3):297-302.
Sakihama, Y., Hasunuma, T., & Kondo, A. (2015). Improved ethanol production from xylose in the presence of acetic acid by the overexpression of the HAA1 gene in Saccharomyces cerevisiae. Journal of Bioscience and Bioengineering, 119(3), 297-302. https://doi.org/10.1016/j.jbiosc.2014.09.004
Sakihama Y, Hasunuma T, Kondo A. Improved Ethanol Production From Xylose in the Presence of Acetic Acid By the Overexpression of the HAA1 Gene in Saccharomyces Cerevisiae. J Biosci Bioeng. 2015;119(3):297-302. PubMed PMID: 25282639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved ethanol production from xylose in the presence of acetic acid by the overexpression of the HAA1 gene in Saccharomyces cerevisiae. AU - Sakihama,Yuri, AU - Hasunuma,Tomohisa, AU - Kondo,Akihiko, Y1 - 2014/10/01/ PY - 2014/06/12/received PY - 2014/08/22/revised PY - 2014/09/02/accepted PY - 2014/10/6/entrez PY - 2014/10/6/pubmed PY - 2015/7/7/medline KW - Acetic acid KW - Ethanol KW - HAA1 KW - Saccharomyces cerevisiae KW - Xylose fermentation SP - 297 EP - 302 JF - Journal of bioscience and bioengineering JO - J Biosci Bioeng VL - 119 IS - 3 N2 - The hydrolysis of lignocellulosic biomass liberates sugars, primarily glucose and xylose, which are subsequently converted to ethanol by microbial fermentation. The rapid and efficient fermentation of xylose by recombinant Saccharomyces cerevisiae strains is limited by weak acids generated during biomass pretreatment processes. In particular, acetic acid negatively affects cell growth, xylose fermentation rate, and ethanol production. The ability of S. cerevisiae to efficiently utilize xylose in the presence of acetic acid is an essential requirement for the cost-effective production of ethanol from lignocellulosic hydrolysates. Here, an acetic acid-responsive transcriptional activator, HAA1, was overexpressed in a recombinant xylose-fermenting S. cerevisiae strain to yield BY4741X/HAA1. This strain exhibited improved cell growth and ethanol production from xylose under aerobic and oxygen limited conditions, respectively, in the presence of acetic acid. The HAA1p regulon enhanced transcript levels in BY4741X/HAA1. The disruption of PHO13, a p-nitrophenylphosphatase gene, in BY4741X/HAA1 led to further improvement in both yeast growth and the ability to ferment xylose, indicating that HAA1 overexpression and PHO13 deletion act by different mechanisms to enhance ethanol production. SN - 1347-4421 UR - https://www.unboundmedicine.com/medline/citation/25282639/Improved_ethanol_production_from_xylose_in_the_presence_of_acetic_acid_by_the_overexpression_of_the_HAA1_gene_in_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -
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