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Correlation of the electrophysiological profiles and sodium channel transcripts of individual rat dorsal root ganglia neurons.
Front Cell Neurosci. 2014; 8:285.FC

Abstract

Voltage gated sodium channels (Nav channels) play an important role in nociceptive transmission. They are intimately tied to the genesis and transmission of neuronal firing. Five different isoforms (Nav1.3, Nav1.6, Nav1.7, Nav1.8, and Nav1.9) have been linked to nociceptive responses. A change in the biophysical properties of these channels or in their expression levels occurs in different pathological pain states. However, the precise involvement of the isoforms in the genesis and transmission of nociceptive responses is unknown. The aim of the present study was to investigate the synergy between the different populations of Nav channels that give individual neurons a unique electrophysical profile. We used the patch-clamp technique in the whole-cell configuration to record Nav currents and action potentials from acutely dissociated small diameter DRG neurons (<30 μm) from adult rats. We also performed single cell qPCR on the same neurons. Our results revealed that there is a strong correlation between Nav currents and mRNA transcripts in individual neurons. A cluster analysis showed that subgroups formed by Nav channel transcripts by mRNA quantification have different biophysical properties. In addition, the firing frequency of the neurons was not affected by the relative populations of Nav channel. The synergy between populations of Nav channel in individual small diameter DRG neurons gives each neuron a unique electrophysiological profile. The Nav channel remodeling that occurs in different pathological pain states may be responsible for the sensitization of the neurons.

Authors+Show Affiliations

Department of Medicine, Centre de Recherche de l'Institut Universitaire en Santé Mentale de Québec, Université Laval Quebec City, QC, Canada.Department of Medicine, Centre de Recherche de l'Institut Universitaire en Santé Mentale de Québec, Université Laval Quebec City, QC, Canada.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25285069

Citation

Thériault, Olivier, and Mohamed Chahine. "Correlation of the Electrophysiological Profiles and Sodium Channel Transcripts of Individual Rat Dorsal Root Ganglia Neurons." Frontiers in Cellular Neuroscience, vol. 8, 2014, p. 285.
Thériault O, Chahine M. Correlation of the electrophysiological profiles and sodium channel transcripts of individual rat dorsal root ganglia neurons. Front Cell Neurosci. 2014;8:285.
Thériault, O., & Chahine, M. (2014). Correlation of the electrophysiological profiles and sodium channel transcripts of individual rat dorsal root ganglia neurons. Frontiers in Cellular Neuroscience, 8, 285. https://doi.org/10.3389/fncel.2014.00285
Thériault O, Chahine M. Correlation of the Electrophysiological Profiles and Sodium Channel Transcripts of Individual Rat Dorsal Root Ganglia Neurons. Front Cell Neurosci. 2014;8:285. PubMed PMID: 25285069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation of the electrophysiological profiles and sodium channel transcripts of individual rat dorsal root ganglia neurons. AU - Thériault,Olivier, AU - Chahine,Mohamed, Y1 - 2014/09/19/ PY - 2014/07/18/received PY - 2014/08/28/accepted PY - 2014/10/7/entrez PY - 2014/10/7/pubmed PY - 2014/10/7/medline KW - biophysical properties KW - dorsal root ganglia neurons KW - neuronal excitability KW - pain KW - voltage-gated sodium channel SP - 285 EP - 285 JF - Frontiers in cellular neuroscience JO - Front Cell Neurosci VL - 8 N2 - Voltage gated sodium channels (Nav channels) play an important role in nociceptive transmission. They are intimately tied to the genesis and transmission of neuronal firing. Five different isoforms (Nav1.3, Nav1.6, Nav1.7, Nav1.8, and Nav1.9) have been linked to nociceptive responses. A change in the biophysical properties of these channels or in their expression levels occurs in different pathological pain states. However, the precise involvement of the isoforms in the genesis and transmission of nociceptive responses is unknown. The aim of the present study was to investigate the synergy between the different populations of Nav channels that give individual neurons a unique electrophysical profile. We used the patch-clamp technique in the whole-cell configuration to record Nav currents and action potentials from acutely dissociated small diameter DRG neurons (<30 μm) from adult rats. We also performed single cell qPCR on the same neurons. Our results revealed that there is a strong correlation between Nav currents and mRNA transcripts in individual neurons. A cluster analysis showed that subgroups formed by Nav channel transcripts by mRNA quantification have different biophysical properties. In addition, the firing frequency of the neurons was not affected by the relative populations of Nav channel. The synergy between populations of Nav channel in individual small diameter DRG neurons gives each neuron a unique electrophysiological profile. The Nav channel remodeling that occurs in different pathological pain states may be responsible for the sensitization of the neurons. SN - 1662-5102 UR - https://www.unboundmedicine.com/medline/citation/25285069/Correlation_of_the_electrophysiological_profiles_and_sodium_channel_transcripts_of_individual_rat_dorsal_root_ganglia_neurons_ L2 - https://doi.org/10.3389/fncel.2014.00285 DB - PRIME DP - Unbound Medicine ER -
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