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New types of wheat chromosomal structural variations in derivatives of wheat-rye hybrids.
PLoS One. 2014; 9(10):e110282.Plos

Abstract

BACKGROUND

Chromosomal rearrangements induced by wheat-rye hybridization is a very well investigated research topic. However, the structural alterations of wheat chromosomes in wheat-rye hybrids are seldom reported.

METHODOLOGY/PRINCIPAL FINDINGS

Octoploid triticale lines were derived from common wheat Triticum. aestivum L. 'Mianyang11'×rye Secale cereale L. 'Kustro'. Some progeny were obtained by the controlled backcrossing of triticale with 'Mianyang11' and common wheat T. aestivum L. 'Chuannong27' followed by self-fertilization. Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) using Oligo-pSc119.2-1, Oligo-pTa535-1 and rye genomic DNA as probes were used to analyze the mitotic chromosomes of these progeny. Alterations of wheat chromosomes including 5A, 6A, 1B, 2B, 6B, 7B, 1D, 3D and 7D were observed. 5AL arm carrying intercalary Oligo-pSc119.2-1, Oligo-pTa535-1 or both Oligo-pSc119.2-1 and Oligo-pTa535-1 signals, 6AS, 1BS and 1DL arms containing terminal Oligo-pSc119.2-1 signal, 6BS and 3DS arms without terminal Oligo-pSc119.2-1 signal, 7BS without subtelomeric Oligo-pSc119.2-1 signal and 7DL with intercalary Oligo-pSc119.2-1 signal have been observed. However, these changed wheat chromosomes have not been detected in 'Mianyang11' and Chuannong 27. The altered 5A, 6A, 7B and 7D chromosomes in this study have not been reported and represent several new karyotype structures of common wheat chromosomes.

CONCLUSIONS/SIGNIFICANCE

These rearranged wheat chromosomes in the present study afford some new genetic variations for wheat breeding program and are valuable materials for studying the biological function of tandem repetitive DNA sequences.

Authors+Show Affiliations

Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.Agronomy College, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25302962

Citation

Tang, Zongxiang, et al. "New Types of Wheat Chromosomal Structural Variations in Derivatives of Wheat-rye Hybrids." PloS One, vol. 9, no. 10, 2014, pp. e110282.
Tang Z, Li M, Chen L, et al. New types of wheat chromosomal structural variations in derivatives of wheat-rye hybrids. PLoS One. 2014;9(10):e110282.
Tang, Z., Li, M., Chen, L., Wang, Y., Ren, Z., & Fu, S. (2014). New types of wheat chromosomal structural variations in derivatives of wheat-rye hybrids. PloS One, 9(10), e110282. https://doi.org/10.1371/journal.pone.0110282
Tang Z, et al. New Types of Wheat Chromosomal Structural Variations in Derivatives of Wheat-rye Hybrids. PLoS One. 2014;9(10):e110282. PubMed PMID: 25302962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New types of wheat chromosomal structural variations in derivatives of wheat-rye hybrids. AU - Tang,Zongxiang, AU - Li,Meng, AU - Chen,Lei, AU - Wang,Yangyang, AU - Ren,Zhenglong, AU - Fu,Shulan, Y1 - 2014/10/10/ PY - 2014/07/31/received PY - 2014/09/15/accepted PY - 2014/10/11/entrez PY - 2014/10/11/pubmed PY - 2015/12/19/medline SP - e110282 EP - e110282 JF - PloS one JO - PLoS One VL - 9 IS - 10 N2 - BACKGROUND: Chromosomal rearrangements induced by wheat-rye hybridization is a very well investigated research topic. However, the structural alterations of wheat chromosomes in wheat-rye hybrids are seldom reported. METHODOLOGY/PRINCIPAL FINDINGS: Octoploid triticale lines were derived from common wheat Triticum. aestivum L. 'Mianyang11'×rye Secale cereale L. 'Kustro'. Some progeny were obtained by the controlled backcrossing of triticale with 'Mianyang11' and common wheat T. aestivum L. 'Chuannong27' followed by self-fertilization. Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) using Oligo-pSc119.2-1, Oligo-pTa535-1 and rye genomic DNA as probes were used to analyze the mitotic chromosomes of these progeny. Alterations of wheat chromosomes including 5A, 6A, 1B, 2B, 6B, 7B, 1D, 3D and 7D were observed. 5AL arm carrying intercalary Oligo-pSc119.2-1, Oligo-pTa535-1 or both Oligo-pSc119.2-1 and Oligo-pTa535-1 signals, 6AS, 1BS and 1DL arms containing terminal Oligo-pSc119.2-1 signal, 6BS and 3DS arms without terminal Oligo-pSc119.2-1 signal, 7BS without subtelomeric Oligo-pSc119.2-1 signal and 7DL with intercalary Oligo-pSc119.2-1 signal have been observed. However, these changed wheat chromosomes have not been detected in 'Mianyang11' and Chuannong 27. The altered 5A, 6A, 7B and 7D chromosomes in this study have not been reported and represent several new karyotype structures of common wheat chromosomes. CONCLUSIONS/SIGNIFICANCE: These rearranged wheat chromosomes in the present study afford some new genetic variations for wheat breeding program and are valuable materials for studying the biological function of tandem repetitive DNA sequences. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25302962/New_types_of_wheat_chromosomal_structural_variations_in_derivatives_of_wheat_rye_hybrids_ L2 - https://dx.plos.org/10.1371/journal.pone.0110282 DB - PRIME DP - Unbound Medicine ER -