TY - JOUR T1 - Evaluating the efficiency of spectral resolution of univariate methods manipulating ratio spectra and comparing to multivariate methods: an application to ternary mixture in common cold preparation. AU - Moustafa,Azza Aziz, AU - Salem,Hesham, AU - Hegazy,Maha, AU - Ali,Omnia, Y1 - 2014/09/28/ PY - 2014/07/02/received PY - 2014/08/16/revised PY - 2014/09/19/accepted PY - 2014/10/13/entrez PY - 2014/10/13/pubmed PY - 2015/8/15/medline KW - Chlorpheniramine maleate KW - Ibuprofen KW - Multivariate methods KW - Pseudoephedrine HCl KW - Ratio spectra KW - Univariate methods SP - 1363 EP - 73 JF - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JO - Spectrochim Acta A Mol Biomol Spectrosc VL - 137 N2 - Simple, accurate, and selective methods have been developed and validated for simultaneous determination of a ternary mixture of Chlorpheniramine maleate (CPM), Pseudoephedrine HCl (PSE) and Ibuprofen (IBF), in tablet dosage form. Four univariate methods manipulating ratio spectra were applied, method A is the double divisor-ratio difference spectrophotometric method (DD-RD). Method B is double divisor-derivative ratio spectrophotometric method (DD-RD). Method C is derivative ratio spectrum-zero crossing method (DRZC), while method D is mean centering of ratio spectra (MCR). Two multivariate methods were also developed and validated, methods E and F are Principal Component Regression (PCR) and Partial Least Squares (PLSs). The proposed methods have the advantage of simultaneous determination of the mentioned drugs without prior separation steps. They were successfully applied to laboratory-prepared mixtures and to commercial pharmaceutical preparation without any interference from additives. The proposed methods were validated according to the ICH guidelines. The obtained results were statistically compared with the official methods where no significant difference was observed regarding both accuracy and precision. SN - 1873-3557 UR - https://www.unboundmedicine.com/medline/citation/25306132/Evaluating_the_efficiency_of_spectral_resolution_of_univariate_methods_manipulating_ratio_spectra_and_comparing_to_multivariate_methods:_an_application_to_ternary_mixture_in_common_cold_preparation_ DB - PRIME DP - Unbound Medicine ER -