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Preparation and characterization of novel electrospun poly(ϵ-caprolactone)-based nanofibrous scaffolds.
Artif Cells Nanomed Biotechnol 2016; 44(2):504-9AC

Abstract

Nanofibrous scaffolds have many advantages that make them excellent candidates for tissue engineering applications. The scaffolds with high surface area to volume ratio favor cell adhesion, proliferation, migration and differentiation. In the present study, the preparation of electrospun poly (ϵ-caprolactone)-polyethylene glycol-poly (ϵ-caprolactone) (PCL-PEG-PCL) nanofibers is shown for the first time. PCL-PEG-PCL copolymers were synthesized using a ring-opening polymerization method. The polymers were characterized by FT-IR, (1)H NMR and DSC. Nanofibers with mean diameters ranging from 60 to 170 nm were obtained by the electrospinning method. Their morphology was evaluated by scanning electron microscopy (SEM). An MTT assay was used to compare the number of cells in the nanofiber scaffold. It was found that the morphology and diameter of the nanofiber depended on the chemical composition and molecular weight of the PEG segment of the copolymer used for electrospinning. Increasing the molecular weight of PEG blocks from 2000 to 6000 led to a decrease of the diameter of the fibers and the formation of beads.

Authors+Show Affiliations

a Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran. b Student Research Committee, Tabriz University of Medical Sciences , Tabriz , Iran. d Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.a Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran. b Student Research Committee, Tabriz University of Medical Sciences , Tabriz , Iran.a Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran. d Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.d Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.a Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran. b Student Research Committee, Tabriz University of Medical Sciences , Tabriz , Iran.a Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.c Department of Clinical Biochemistry , Faculty of Medicine, Tabriz University of Medical Sciences , Tabriz , Iran.a Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran. d Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25307268

Citation

Valizadeh, Alireza, et al. "Preparation and Characterization of Novel Electrospun Poly(ϵ-caprolactone)-based Nanofibrous Scaffolds." Artificial Cells, Nanomedicine, and Biotechnology, vol. 44, no. 2, 2016, pp. 504-9.
Valizadeh A, Bakhtiary M, Akbarzadeh A, et al. Preparation and characterization of novel electrospun poly(ϵ-caprolactone)-based nanofibrous scaffolds. Artif Cells Nanomed Biotechnol. 2016;44(2):504-9.
Valizadeh, A., Bakhtiary, M., Akbarzadeh, A., Salehi, R., Frakhani, S. M., Ebrahimi, O., ... Davaran, S. (2016). Preparation and characterization of novel electrospun poly(ϵ-caprolactone)-based nanofibrous scaffolds. Artificial Cells, Nanomedicine, and Biotechnology, 44(2), pp. 504-9. doi:10.3109/21691401.2014.965310.
Valizadeh A, et al. Preparation and Characterization of Novel Electrospun Poly(ϵ-caprolactone)-based Nanofibrous Scaffolds. Artif Cells Nanomed Biotechnol. 2016;44(2):504-9. PubMed PMID: 25307268.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation and characterization of novel electrospun poly(ϵ-caprolactone)-based nanofibrous scaffolds. AU - Valizadeh,Alireza, AU - Bakhtiary,Mohsen, AU - Akbarzadeh,Abolfazl, AU - Salehi,Roya, AU - Frakhani,Samad Mussa, AU - Ebrahimi,Ommolbanin, AU - Rahmati-yamchi,Mohammad, AU - Davaran,Soodabeh, Y1 - 2014/10/13/ PY - 2014/10/14/entrez PY - 2014/10/14/pubmed PY - 2016/11/2/medline KW - MTT assay KW - PCL-PEG-PCL KW - electrospinning KW - nanofibrous scaffolds SP - 504 EP - 9 JF - Artificial cells, nanomedicine, and biotechnology JO - Artif Cells Nanomed Biotechnol VL - 44 IS - 2 N2 - Nanofibrous scaffolds have many advantages that make them excellent candidates for tissue engineering applications. The scaffolds with high surface area to volume ratio favor cell adhesion, proliferation, migration and differentiation. In the present study, the preparation of electrospun poly (ϵ-caprolactone)-polyethylene glycol-poly (ϵ-caprolactone) (PCL-PEG-PCL) nanofibers is shown for the first time. PCL-PEG-PCL copolymers were synthesized using a ring-opening polymerization method. The polymers were characterized by FT-IR, (1)H NMR and DSC. Nanofibers with mean diameters ranging from 60 to 170 nm were obtained by the electrospinning method. Their morphology was evaluated by scanning electron microscopy (SEM). An MTT assay was used to compare the number of cells in the nanofiber scaffold. It was found that the morphology and diameter of the nanofiber depended on the chemical composition and molecular weight of the PEG segment of the copolymer used for electrospinning. Increasing the molecular weight of PEG blocks from 2000 to 6000 led to a decrease of the diameter of the fibers and the formation of beads. SN - 2169-141X UR - https://www.unboundmedicine.com/medline/citation/25307268/Preparation_and_characterization_of_novel_electrospun_poly_ϵ_caprolactone__based_nanofibrous_scaffolds_ L2 - http://www.tandfonline.com/doi/full/10.3109/21691401.2014.965310 DB - PRIME DP - Unbound Medicine ER -