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Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
J Sci Food Agric. 2015 Aug 30; 95(11):2337-44.JS

Abstract

BACKGROUND

Green tea is a dietary source of bioactive compounds for human health. Enzymatic treatments induce the bioconversion of bioactive components, which can improve biological activities. In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).

RESULTS

Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content. The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG). Biotransformation of catechins improved the radical scavenging activity of GTE. Pectinase treatment led to change of TPS composition in GTE by hydrolyzing polysaccharides. In addition, pectinase-driven hydrolysis in polysaccharides significantly increased TPS-induced Interleukin 6 (IL-6) production in macrophages. In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.

CONCLUSION

Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.

Authors+Show Affiliations

Cosmax Bio INC, Jecheon, 390-250, Republic of Korea.Department of Food Science and Biotechnology, Kyonggi University, Suwon, 443-760, Republic of Korea.Department of Food and Nutrition, Korea University, Seoul, 136-703, Republic of Korea.Department of Food and Nutrition, Korea National University of Transportation, Jeungpyeong, 368-701, Republic of Korea.Department of Food and Nutrition, Korea University, Seoul, 136-703, Republic of Korea.Department of Food Science and Technology, Seoul Women's University, Seoul, 139-774, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25307474

Citation

Baik, Joo Hyun, et al. "Biotransformation of Catechin and Extraction of Active Polysaccharide From Green Tea Leaves Via Simultaneous Treatment With Tannase and Pectinase." Journal of the Science of Food and Agriculture, vol. 95, no. 11, 2015, pp. 2337-44.
Baik JH, Shin KS, Park Y, et al. Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase. J Sci Food Agric. 2015;95(11):2337-44.
Baik, J. H., Shin, K. S., Park, Y., Yu, K. W., Suh, H. J., & Choi, H. S. (2015). Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase. Journal of the Science of Food and Agriculture, 95(11), 2337-44. https://doi.org/10.1002/jsfa.6955
Baik JH, et al. Biotransformation of Catechin and Extraction of Active Polysaccharide From Green Tea Leaves Via Simultaneous Treatment With Tannase and Pectinase. J Sci Food Agric. 2015 Aug 30;95(11):2337-44. PubMed PMID: 25307474.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase. AU - Baik,Joo Hyun, AU - Shin,Kwang-Soon, AU - Park,Yooheon, AU - Yu,Kwang-Won, AU - Suh,Hyung Joo, AU - Choi,Hyeon-Son, Y1 - 2014/11/07/ PY - 2014/07/30/received PY - 2014/09/23/revised PY - 2014/10/08/accepted PY - 2014/10/14/entrez PY - 2014/10/14/pubmed PY - 2016/4/19/medline KW - IL-6 production KW - biotransformation KW - radical scavenging activity KW - simultaneous enzymatic process KW - tea polysaccharide SP - 2337 EP - 44 JF - Journal of the science of food and agriculture JO - J Sci Food Agric VL - 95 IS - 11 N2 - BACKGROUND: Green tea is a dietary source of bioactive compounds for human health. Enzymatic treatments induce the bioconversion of bioactive components, which can improve biological activities. In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE). RESULTS: Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content. The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG). Biotransformation of catechins improved the radical scavenging activity of GTE. Pectinase treatment led to change of TPS composition in GTE by hydrolyzing polysaccharides. In addition, pectinase-driven hydrolysis in polysaccharides significantly increased TPS-induced Interleukin 6 (IL-6) production in macrophages. In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control. CONCLUSION: Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/25307474/Biotransformation_of_catechin_and_extraction_of_active_polysaccharide_from_green_tea_leaves_via_simultaneous_treatment_with_tannase_and_pectinase_ L2 - https://doi.org/10.1002/jsfa.6955 DB - PRIME DP - Unbound Medicine ER -