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Evaluation of the genotoxicity of a herbicide formulation containing 3,6-dichloro-2-metoxybenzoic acid (dicamba) in circulating blood cells of the tropical fish Cnesterodon decemmaculatus.

Abstract

Acute toxicity and genotoxicity of the dicamba-based commercial herbicide formulation Banvel(®) were evaluated on Cnesterodon decemmaculatus (Pisces, Poeciliidae) exposed under laboratory conditions. A lethal effect was used as the end point for mortality, whereas frequency of micronuclei (MNs) and DNA single-strand breaks evaluated by the single cell gel electrophoresis assay were employed as end points for genotoxicity. Mortality studies revealed an LC50 96 h value of 1639 mg/L (range, 1471-1808) of dicamba. Furthermore, behavioral changes, e.g., gathering at the bottom of the aquarium, slowness in motion, abnormal swimming, and slow reaction, were observed. Whereas increased frequency of MNs was observed when 1229 mg/L dicamba was assayed for 48 h, no induction of MNs was observed in fish exposed to the herbicide for 96 h, regardless of the concentration of dicamba. Furthermore, other nuclear abnormalities, i.e., binucleated cells and lobed and notched nuclei, were induced in fish exposed for 48 h but not 96 h. Increase in the genetic damage index was observed in those treatments (lasting for both 48 and 96 h) within the 410-1229 mg/L dicamba concentration-range. This study represents the first evidence of acute lethal and sublethal effects exerted by dicamba on a piscine species native to Argentina. The results could indicate that dicamba-based formulation Banvel(®) is the less toxic emerging pollutant reported so far for C. decemmaculatus. Finally, our findings highlight the properties of this herbicide that jeopardize nontarget living species exposed to this agrochemical.

Authors+Show Affiliations

Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 N° 3, B1904AMA La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: marcelo.larramendy@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25308699

Citation

Ruiz de Arcaute, C, et al. "Evaluation of the Genotoxicity of a Herbicide Formulation Containing 3,6-dichloro-2-metoxybenzoic Acid (dicamba) in Circulating Blood Cells of the Tropical Fish Cnesterodon Decemmaculatus." Mutation Research. Genetic Toxicology and Environmental Mutagenesis, vol. 773, 2014, pp. 1-8.
Ruiz de Arcaute C, Soloneski S, Larramendy ML. Evaluation of the genotoxicity of a herbicide formulation containing 3,6-dichloro-2-metoxybenzoic acid (dicamba) in circulating blood cells of the tropical fish Cnesterodon decemmaculatus. Mutat Res Genet Toxicol Environ Mutagen. 2014;773:1-8.
Ruiz de Arcaute, C., Soloneski, S., & Larramendy, M. L. (2014). Evaluation of the genotoxicity of a herbicide formulation containing 3,6-dichloro-2-metoxybenzoic acid (dicamba) in circulating blood cells of the tropical fish Cnesterodon decemmaculatus. Mutation Research. Genetic Toxicology and Environmental Mutagenesis, 773, 1-8. https://doi.org/10.1016/j.mrgentox.2014.08.001
Ruiz de Arcaute C, Soloneski S, Larramendy ML. Evaluation of the Genotoxicity of a Herbicide Formulation Containing 3,6-dichloro-2-metoxybenzoic Acid (dicamba) in Circulating Blood Cells of the Tropical Fish Cnesterodon Decemmaculatus. Mutat Res Genet Toxicol Environ Mutagen. 2014;773:1-8. PubMed PMID: 25308699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the genotoxicity of a herbicide formulation containing 3,6-dichloro-2-metoxybenzoic acid (dicamba) in circulating blood cells of the tropical fish Cnesterodon decemmaculatus. AU - Ruiz de Arcaute,C, AU - Soloneski,S, AU - Larramendy,M L, Y1 - 2014/08/19/ PY - 2014/06/23/received PY - 2014/08/07/revised PY - 2014/08/08/accepted PY - 2014/10/14/entrez PY - 2014/10/14/pubmed PY - 2015/1/3/medline KW - Banvel(®) KW - Dicamba-based commercial formulation KW - Lethality KW - Micronuclei KW - SCGE assay KW - Sublethal effects SP - 1 EP - 8 JF - Mutation research. Genetic toxicology and environmental mutagenesis JO - Mutat Res Genet Toxicol Environ Mutagen VL - 773 N2 - Acute toxicity and genotoxicity of the dicamba-based commercial herbicide formulation Banvel(®) were evaluated on Cnesterodon decemmaculatus (Pisces, Poeciliidae) exposed under laboratory conditions. A lethal effect was used as the end point for mortality, whereas frequency of micronuclei (MNs) and DNA single-strand breaks evaluated by the single cell gel electrophoresis assay were employed as end points for genotoxicity. Mortality studies revealed an LC50 96 h value of 1639 mg/L (range, 1471-1808) of dicamba. Furthermore, behavioral changes, e.g., gathering at the bottom of the aquarium, slowness in motion, abnormal swimming, and slow reaction, were observed. Whereas increased frequency of MNs was observed when 1229 mg/L dicamba was assayed for 48 h, no induction of MNs was observed in fish exposed to the herbicide for 96 h, regardless of the concentration of dicamba. Furthermore, other nuclear abnormalities, i.e., binucleated cells and lobed and notched nuclei, were induced in fish exposed for 48 h but not 96 h. Increase in the genetic damage index was observed in those treatments (lasting for both 48 and 96 h) within the 410-1229 mg/L dicamba concentration-range. This study represents the first evidence of acute lethal and sublethal effects exerted by dicamba on a piscine species native to Argentina. The results could indicate that dicamba-based formulation Banvel(®) is the less toxic emerging pollutant reported so far for C. decemmaculatus. Finally, our findings highlight the properties of this herbicide that jeopardize nontarget living species exposed to this agrochemical. SN - 1879-3592 UR - https://www.unboundmedicine.com/medline/citation/25308699/Evaluation_of_the_genotoxicity_of_a_herbicide_formulation_containing_36_dichloro_2_metoxybenzoic_acid__dicamba__in_circulating_blood_cells_of_the_tropical_fish_Cnesterodon_decemmaculatus_ DB - PRIME DP - Unbound Medicine ER -