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Ultrasound-assisted extraction of phenolic compounds from Cratoxylum formosum ssp. formosum leaves using central composite design and evaluation of its protective ability against H2O2-induced cell death.
Asian Pac J Trop Med 2014; 7S1:S497-505AP

Abstract

OBJECTIVE

To optimize the processing parameters for phenolic compounds extracted from Cratoxylum formosum ssp. formosum leaves using an ultrasound-assisted extraction and to evaluate its protective ability against H2O2-induced cell death.

METHODS

The influence of three independent variables including ethanol concentration (%), extraction temperature (°C) and extraction time (min) on the extraction yield of phenolic compounds were optimized using a central composite design-based response surface methodology. The obtained extract was assessed for its antioxidant activity by DPPH(•) and ABTS(•)(+) methods. Cellular protective ability against H2O2-induced cell death was evaluated on HEK293 cells using the MTT assay.

RESULTS

The optimal conditions to achieve maximal yields of phenolic compounds were ethanol concentration of 50.33% (v/v), temperature of 65 °C, and extractiontion time of 15 min. The yield of phenolic compounds was (40.00±1.00) mg gallic acid equivalent/g dry powder which matched well with the values predicted from the proposed model. These conditions resulted in a higher efficiency concerning the extraction of phenolics compared to a conventional heat reflux extraction by providing shorter extraction time and reduced energy consumption. 5-O-caffeoylquinic acid identified by high performance liquid chromatography-diode array detector-electron spin ionization-mass spectrometry was the major compound in the obtained extract [(41.66±0.07) mg/g plant extract]. The obtained extract showed a strong ability to scavenge both DPPH(•) and ABTS(•)(+) free radicals and exhibited additionally good ability to protect HEK293 cells death against oxidative stress.

CONCLUSIONS

These results indicate the suitability of ultrasound-assisted extraction for the extraction of phenolic compounds from Cratoxylum formosum ssp. formosum leaves. This phenolic-enriched extract can be used as valuable antioxidant source for health benefits.

Authors+Show Affiliations

Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4/1, A-8010 Graz, Austria; Faculty of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry and Technology, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4/1, A-8010 Graz, Austria.Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4/1, A-8010 Graz, Austria. Electronic address: adelheid.brantner@uni-graz.at.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25312174

Citation

Yingngam, Bancha, et al. "Ultrasound-assisted Extraction of Phenolic Compounds From Cratoxylum Formosum Ssp. Formosum Leaves Using Central Composite Design and Evaluation of Its Protective Ability Against H2O2-induced Cell Death." Asian Pacific Journal of Tropical Medicine, vol. 7S1, 2014, pp. S497-505.
Yingngam B, Monschein M, Brantner A. Ultrasound-assisted extraction of phenolic compounds from Cratoxylum formosum ssp. formosum leaves using central composite design and evaluation of its protective ability against H2O2-induced cell death. Asian Pac J Trop Med. 2014;7S1:S497-505.
Yingngam, B., Monschein, M., & Brantner, A. (2014). Ultrasound-assisted extraction of phenolic compounds from Cratoxylum formosum ssp. formosum leaves using central composite design and evaluation of its protective ability against H2O2-induced cell death. Asian Pacific Journal of Tropical Medicine, 7S1, pp. S497-505. doi:10.1016/S1995-7645(14)60281-9.
Yingngam B, Monschein M, Brantner A. Ultrasound-assisted Extraction of Phenolic Compounds From Cratoxylum Formosum Ssp. Formosum Leaves Using Central Composite Design and Evaluation of Its Protective Ability Against H2O2-induced Cell Death. Asian Pac J Trop Med. 2014;7S1:S497-505. PubMed PMID: 25312174.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasound-assisted extraction of phenolic compounds from Cratoxylum formosum ssp. formosum leaves using central composite design and evaluation of its protective ability against H2O2-induced cell death. AU - Yingngam,Bancha, AU - Monschein,Marlene, AU - Brantner,Adelheid, PY - 2014/04/13/received PY - 2014/05/23/revised PY - 2014/10/15/entrez PY - 2014/10/15/pubmed PY - 2014/10/15/medline KW - Antioxidants KW - Cratoxylum formosum ssp. formosum KW - Optimization KW - Oxidative stress KW - Phenolics KW - Response surface methodology KW - Ultrasound-assisted extraction SP - S497 EP - 505 JF - Asian Pacific journal of tropical medicine JO - Asian Pac J Trop Med VL - 7S1 N2 - OBJECTIVE: To optimize the processing parameters for phenolic compounds extracted from Cratoxylum formosum ssp. formosum leaves using an ultrasound-assisted extraction and to evaluate its protective ability against H2O2-induced cell death. METHODS: The influence of three independent variables including ethanol concentration (%), extraction temperature (°C) and extraction time (min) on the extraction yield of phenolic compounds were optimized using a central composite design-based response surface methodology. The obtained extract was assessed for its antioxidant activity by DPPH(•) and ABTS(•)(+) methods. Cellular protective ability against H2O2-induced cell death was evaluated on HEK293 cells using the MTT assay. RESULTS: The optimal conditions to achieve maximal yields of phenolic compounds were ethanol concentration of 50.33% (v/v), temperature of 65 °C, and extractiontion time of 15 min. The yield of phenolic compounds was (40.00±1.00) mg gallic acid equivalent/g dry powder which matched well with the values predicted from the proposed model. These conditions resulted in a higher efficiency concerning the extraction of phenolics compared to a conventional heat reflux extraction by providing shorter extraction time and reduced energy consumption. 5-O-caffeoylquinic acid identified by high performance liquid chromatography-diode array detector-electron spin ionization-mass spectrometry was the major compound in the obtained extract [(41.66±0.07) mg/g plant extract]. The obtained extract showed a strong ability to scavenge both DPPH(•) and ABTS(•)(+) free radicals and exhibited additionally good ability to protect HEK293 cells death against oxidative stress. CONCLUSIONS: These results indicate the suitability of ultrasound-assisted extraction for the extraction of phenolic compounds from Cratoxylum formosum ssp. formosum leaves. This phenolic-enriched extract can be used as valuable antioxidant source for health benefits. SN - 2352-4146 UR - https://www.unboundmedicine.com/medline/citation/25312174/Ultrasound_assisted_extraction_of_phenolic_compounds_from_Cratoxylum_formosum_ssp__formosum_leaves_using_central_composite_design_and_evaluation_of_its_protective_ability_against_H2O2_induced_cell_death_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1995-7645(14)60281-9 DB - PRIME DP - Unbound Medicine ER -