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Characterization of paraquat-induced miRNA profiling response in hNPCs undergoing proliferation.
Int J Mol Sci. 2014 Oct 13; 15(10):18422-36.IJ

Abstract

Aberration during the development of the central nervous system (CNS) due to environmental factors underlies a variety of adverse developmental outcomes. Paraquat (PQ) is a widely studied neurotoxicant that perturbs the normal structure/function of adult CNS. Yet, the impacts of PQ exposure on the developing CNS remain unclear. miRNAs represent a class of small non-coding RNA molecules involved in the regulation of neural development. Thus in the present study, we analyzed the impacts of PQ on the miRNome of human neural progenitor cells (hNPCs) during proliferation by using the Exiqon miRCURY™ LNA Array. A total of 66 miRNAs were identified as differentially expressed in proliferating hNPCs upon PQ treatment. miRTarBase prediction identified 1465 mRNAs, including several genes (e.g., nestin, sox1, ngn1) previously proved to be associated with the neural proliferation and differentiation, as target genes of PQ-induced differentially expressed miRNAs. The database for annotation, visualization and integrated discovery (DAVID) bioinformatics analysis showed that target genes were enriched in regulation of cell proliferation and differentiation, cell cycle and apoptosis as well as tumor protein 53 (p53), Wnt, Notch and mitogen-activated protein kinases (MAPK) signaling pathways (p < 0.001). These findings were confirmed by real-time RT-PCR. Based on our results we conclude that PQ-induced impacts on the miRNA profiling of hNPCs undergoing proliferation may underlie the developmental neurotoxicity of PQ.

Authors+Show Affiliations

School of Public Health/MOE Key Lab of Public Health Safety/WHO Collaborating Center for Occupational Health, Fudan University, Shanghai 200032, China. clever_hmin@aliyun.com.School of Public Health/MOE Key Lab of Public Health Safety/WHO Collaborating Center for Occupational Health, Fudan University, Shanghai 200032, China. delicia.ldan@gmail.com.Department of Occupational and Environmental Health, School of Public Health, Ningxia Medical University, Yinchuan 750000, China. caiqian1212@126.com.School of Public Health/MOE Key Lab of Public Health Safety/WHO Collaborating Center for Occupational Health, Fudan University, Shanghai 200032, China. xlchang@shmu.edu.cn.School of Public Health/MOE Key Lab of Public Health Safety/WHO Collaborating Center for Occupational Health, Fudan University, Shanghai 200032, China. 12211020063@fudan.edu.cn.School of Public Health/MOE Key Lab of Public Health Safety/WHO Collaborating Center for Occupational Health, Fudan University, Shanghai 200032, China. zjzhou@fudan.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25314302

Citation

Huang, Min, et al. "Characterization of Paraquat-induced miRNA Profiling Response in hNPCs Undergoing Proliferation." International Journal of Molecular Sciences, vol. 15, no. 10, 2014, pp. 18422-36.
Huang M, Lou D, Cai Q, et al. Characterization of paraquat-induced miRNA profiling response in hNPCs undergoing proliferation. Int J Mol Sci. 2014;15(10):18422-36.
Huang, M., Lou, D., Cai, Q., Chang, X., Wang, X., & Zhou, Z. (2014). Characterization of paraquat-induced miRNA profiling response in hNPCs undergoing proliferation. International Journal of Molecular Sciences, 15(10), 18422-36. https://doi.org/10.3390/ijms151018422
Huang M, et al. Characterization of Paraquat-induced miRNA Profiling Response in hNPCs Undergoing Proliferation. Int J Mol Sci. 2014 Oct 13;15(10):18422-36. PubMed PMID: 25314302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of paraquat-induced miRNA profiling response in hNPCs undergoing proliferation. AU - Huang,Min, AU - Lou,Dan, AU - Cai,Qian, AU - Chang,Xiuli, AU - Wang,Xinjin, AU - Zhou,Zhijun, Y1 - 2014/10/13/ PY - 2014/08/21/received PY - 2014/09/26/revised PY - 2014/09/29/accepted PY - 2014/10/15/entrez PY - 2014/10/15/pubmed PY - 2015/6/26/medline SP - 18422 EP - 36 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 15 IS - 10 N2 - Aberration during the development of the central nervous system (CNS) due to environmental factors underlies a variety of adverse developmental outcomes. Paraquat (PQ) is a widely studied neurotoxicant that perturbs the normal structure/function of adult CNS. Yet, the impacts of PQ exposure on the developing CNS remain unclear. miRNAs represent a class of small non-coding RNA molecules involved in the regulation of neural development. Thus in the present study, we analyzed the impacts of PQ on the miRNome of human neural progenitor cells (hNPCs) during proliferation by using the Exiqon miRCURY™ LNA Array. A total of 66 miRNAs were identified as differentially expressed in proliferating hNPCs upon PQ treatment. miRTarBase prediction identified 1465 mRNAs, including several genes (e.g., nestin, sox1, ngn1) previously proved to be associated with the neural proliferation and differentiation, as target genes of PQ-induced differentially expressed miRNAs. The database for annotation, visualization and integrated discovery (DAVID) bioinformatics analysis showed that target genes were enriched in regulation of cell proliferation and differentiation, cell cycle and apoptosis as well as tumor protein 53 (p53), Wnt, Notch and mitogen-activated protein kinases (MAPK) signaling pathways (p < 0.001). These findings were confirmed by real-time RT-PCR. Based on our results we conclude that PQ-induced impacts on the miRNA profiling of hNPCs undergoing proliferation may underlie the developmental neurotoxicity of PQ. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/25314302/Characterization_of_paraquat_induced_miRNA_profiling_response_in_hNPCs_undergoing_proliferation_ L2 - http://www.mdpi.com/resolver?pii=ijms151018422 DB - PRIME DP - Unbound Medicine ER -