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Construction of photoelectrochemical thrombin aptasensor via assembling multilayer of graphene-CdS nanocomposites.
Biosens Bioelectron. 2015 Feb 15; 64:611-7.BB

Abstract

A photoelectrochemical (PEC) aptasensor for highly sensitive and specific detection of thrombin was developed by using graphene–CdS nanocomposites multilayer as photoactive species and electroactive mediator hexaammineruthenium(III) chloride (Ru(NH(3))(6)(3+)) as signal enhancer. Graphene–CdS nanocomposites (G–CdS) were synthesized by one-pot reduction of oxide graphene and CdCl2 with thioacetamide. The photoactive multilayer was prepared by alternative assembly of the negatively charged 3-mercaptopropionic acid modified graphene–CdS nanocomposites (MPA-G–CdS) and the positively charged polyethylenimine (PEI) on ITO electrode. This layer-by-layer assembly method enhanced the stability and homogeneity of the photocurrent readout of G–CdS. Thrombin aptamer was covalently bound to the multilayer by using glutaraldehyde as cross-linking. Electroactive mediator (Ru(NH(3))(6)(3+)) could interact with the DNA phosphate backbone and thus facilitated the electron transfer between G–CdS multilayer and electrode and enhanced the photocurrent. Hybridizing of a long complementary DNA with thrombin aptamer could increase the adsorption amount of (Ru(NH(3))(6)(3+)), which in turn boosted the signal readout. In the presence of target thrombin, the affinity interaction between thrombin and its aptamer resulted in the long complementary DNA releasing from the G–CdS multilayer and decreasing of photocurrent signal. On the basis of G–CdS multilayer as the photoactive species, (Ru (NH(3))(6)(3+)) as an electroactive mediator, and aptamer as a recognition module, a high sensitive PEC aptasensor for thrombin detection was proposed. The thrombin aptasensor displayed a linear range from 2.0 pM to 600.0 pM and a detection limit of 1.0 pM. The present strategy provided a promising ideology for the future development of PEC biosensor.

Authors+Show Affiliations

Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210096, PR China.Jingjiang People's Hospital, PR China.Jingjiang People's Hospital, PR China.Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210096, PR China; Suzhou Research Institute of Southeast University, PR China. Electronic address: liusq@seu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25314620

Citation

Shangguan, Li, et al. "Construction of Photoelectrochemical Thrombin Aptasensor Via Assembling Multilayer of graphene-CdS Nanocomposites." Biosensors & Bioelectronics, vol. 64, 2015, pp. 611-7.
Shangguan L, Zhu W, Xue Y, et al. Construction of photoelectrochemical thrombin aptasensor via assembling multilayer of graphene-CdS nanocomposites. Biosens Bioelectron. 2015;64:611-7.
Shangguan, L., Zhu, W., Xue, Y., & Liu, S. (2015). Construction of photoelectrochemical thrombin aptasensor via assembling multilayer of graphene-CdS nanocomposites. Biosensors & Bioelectronics, 64, 611-7. https://doi.org/10.1016/j.bios.2014.09.072
Shangguan L, et al. Construction of Photoelectrochemical Thrombin Aptasensor Via Assembling Multilayer of graphene-CdS Nanocomposites. Biosens Bioelectron. 2015 Feb 15;64:611-7. PubMed PMID: 25314620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of photoelectrochemical thrombin aptasensor via assembling multilayer of graphene-CdS nanocomposites. AU - Shangguan,Li, AU - Zhu,Wei, AU - Xue,Yanchun, AU - Liu,Songqin, Y1 - 2014/09/28/ PY - 2014/07/05/received PY - 2014/09/25/revised PY - 2014/09/25/accepted PY - 2014/10/15/entrez PY - 2014/10/15/pubmed PY - 2015/7/24/medline KW - Aptamer KW - Graphene–CdS KW - Hexaammineruthenium(III) chloride KW - Photoelectrochemical KW - Thrombin SP - 611 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 64 N2 - A photoelectrochemical (PEC) aptasensor for highly sensitive and specific detection of thrombin was developed by using graphene–CdS nanocomposites multilayer as photoactive species and electroactive mediator hexaammineruthenium(III) chloride (Ru(NH(3))(6)(3+)) as signal enhancer. Graphene–CdS nanocomposites (G–CdS) were synthesized by one-pot reduction of oxide graphene and CdCl2 with thioacetamide. The photoactive multilayer was prepared by alternative assembly of the negatively charged 3-mercaptopropionic acid modified graphene–CdS nanocomposites (MPA-G–CdS) and the positively charged polyethylenimine (PEI) on ITO electrode. This layer-by-layer assembly method enhanced the stability and homogeneity of the photocurrent readout of G–CdS. Thrombin aptamer was covalently bound to the multilayer by using glutaraldehyde as cross-linking. Electroactive mediator (Ru(NH(3))(6)(3+)) could interact with the DNA phosphate backbone and thus facilitated the electron transfer between G–CdS multilayer and electrode and enhanced the photocurrent. Hybridizing of a long complementary DNA with thrombin aptamer could increase the adsorption amount of (Ru(NH(3))(6)(3+)), which in turn boosted the signal readout. In the presence of target thrombin, the affinity interaction between thrombin and its aptamer resulted in the long complementary DNA releasing from the G–CdS multilayer and decreasing of photocurrent signal. On the basis of G–CdS multilayer as the photoactive species, (Ru (NH(3))(6)(3+)) as an electroactive mediator, and aptamer as a recognition module, a high sensitive PEC aptasensor for thrombin detection was proposed. The thrombin aptasensor displayed a linear range from 2.0 pM to 600.0 pM and a detection limit of 1.0 pM. The present strategy provided a promising ideology for the future development of PEC biosensor. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/25314620/Construction_of_photoelectrochemical_thrombin_aptasensor_via_assembling_multilayer_of_graphene_CdS_nanocomposites_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(14)00759-3 DB - PRIME DP - Unbound Medicine ER -