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Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis.
J Dermatol Sci. 2014 Dec; 76(3):231-9.JD

Abstract

BACKGROUND

Dry skin in atopic dermatitis (AD) mainly results from barrier impairment due to deficiency of ceramide and natural moisturizing factors including pyrrolidone carboxylic acid (PCA) in stratum corneum (SC). Caspase-14 cleaves filaggrin monomers to free amino acids and their derivatives such as PCA, contributing natural moisturizing factors. Cytokines in the corneocytes represent cutaneous inflammation severity of AD patients.

OBJECT

To analyze the correlations of PCA, caspase-14 and cytokines in corneocytes with clinical severity, barrier function and skin inflammation, those were quantitated.

METHODS

A total of 73 persons were enrolled: 21 patients with mild AD, 21 with moderate-to-severe AD, 13 with X-linked ichthyosis (XLI) as a negative control for filaggrin gene (FLG) mutation, and 18 healthy controls. Skin barrier functions such as basal transepidermal water loss (TEWL), stratum corneum (SC) hydration and skin surface pH were measured. To collect corneocytes, stripping with D-squame discs was done on lesional and non-lesional skin. And then PCA was isolated from D-squame discs and quantitated by LC-MS/MS. Cytokine assays were performed.

RESULTS

The quantity of PCA and caspase-14 was decreased in inflammatory lesions compared to non-lesion in AD patients. And the amounts of PCA and caspase-14 in the lesion of AD patients correlated with clinical severity as determined by eczema area and severity index score and the skin barrier functions. Also, the expressions of TNF-α and IL-13 inversely correlated with PCA quantity.

CONCLUSION

The quantity of PCA or caspase-14 in the corneocytes of the lesional skin of AD patients reflects the clinical severity, skin barrier function and the degree of lesional inflammation.

Authors+Show Affiliations

Department of Dermatology, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju, 220-701 Korea.Department of Dermatology, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju, 220-701 Korea.Seoul Medical Center Research Institute, Seoul, Korea.Seoul Medical Center Research Institute, Seoul, Korea; Atopy Asthma Center/Department of Dermatology, Seoul Medical Center, Seoul, Korea.WCI Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Korea.Department of Dermatology, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju, 220-701 Korea. Electronic address: choieh@yonsei.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25315296

Citation

Jung, Minyoung, et al. "Pyrrolidone Carboxylic Acid Levels or Caspase-14 Expression in the Corneocytes of Lesional Skin Correlates With Clinical Severity, Skin Barrier Function and Lesional Inflammation in Atopic Dermatitis." Journal of Dermatological Science, vol. 76, no. 3, 2014, pp. 231-9.
Jung M, Choi J, Lee SA, et al. Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis. J Dermatol Sci. 2014;76(3):231-9.
Jung, M., Choi, J., Lee, S. A., Kim, H., Hwang, J., & Choi, E. H. (2014). Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis. Journal of Dermatological Science, 76(3), 231-9. https://doi.org/10.1016/j.jdermsci.2014.09.004
Jung M, et al. Pyrrolidone Carboxylic Acid Levels or Caspase-14 Expression in the Corneocytes of Lesional Skin Correlates With Clinical Severity, Skin Barrier Function and Lesional Inflammation in Atopic Dermatitis. J Dermatol Sci. 2014;76(3):231-9. PubMed PMID: 25315296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis. AU - Jung,Minyoung, AU - Choi,Jaewoong, AU - Lee,Seon-Ah, AU - Kim,Hyunjung, AU - Hwang,Joonsung, AU - Choi,Eung Ho, Y1 - 2014/09/30/ PY - 2014/05/07/received PY - 2014/09/06/revised PY - 2014/09/18/accepted PY - 2014/10/16/entrez PY - 2014/10/16/pubmed PY - 2015/8/5/medline KW - Atopic dermatitis KW - Caspase-14 KW - Filaggrin KW - Natural moisturizing factor KW - Pyrrolidone carboxylic acid SP - 231 EP - 9 JF - Journal of dermatological science JO - J. Dermatol. Sci. VL - 76 IS - 3 N2 - BACKGROUND: Dry skin in atopic dermatitis (AD) mainly results from barrier impairment due to deficiency of ceramide and natural moisturizing factors including pyrrolidone carboxylic acid (PCA) in stratum corneum (SC). Caspase-14 cleaves filaggrin monomers to free amino acids and their derivatives such as PCA, contributing natural moisturizing factors. Cytokines in the corneocytes represent cutaneous inflammation severity of AD patients. OBJECT: To analyze the correlations of PCA, caspase-14 and cytokines in corneocytes with clinical severity, barrier function and skin inflammation, those were quantitated. METHODS: A total of 73 persons were enrolled: 21 patients with mild AD, 21 with moderate-to-severe AD, 13 with X-linked ichthyosis (XLI) as a negative control for filaggrin gene (FLG) mutation, and 18 healthy controls. Skin barrier functions such as basal transepidermal water loss (TEWL), stratum corneum (SC) hydration and skin surface pH were measured. To collect corneocytes, stripping with D-squame discs was done on lesional and non-lesional skin. And then PCA was isolated from D-squame discs and quantitated by LC-MS/MS. Cytokine assays were performed. RESULTS: The quantity of PCA and caspase-14 was decreased in inflammatory lesions compared to non-lesion in AD patients. And the amounts of PCA and caspase-14 in the lesion of AD patients correlated with clinical severity as determined by eczema area and severity index score and the skin barrier functions. Also, the expressions of TNF-α and IL-13 inversely correlated with PCA quantity. CONCLUSION: The quantity of PCA or caspase-14 in the corneocytes of the lesional skin of AD patients reflects the clinical severity, skin barrier function and the degree of lesional inflammation. SN - 1873-569X UR - https://www.unboundmedicine.com/medline/citation/25315296/Pyrrolidone_carboxylic_acid_levels_or_caspase_14_expression_in_the_corneocytes_of_lesional_skin_correlates_with_clinical_severity_skin_barrier_function_and_lesional_inflammation_in_atopic_dermatitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-1811(14)00221-7 DB - PRIME DP - Unbound Medicine ER -