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Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts.
Int J Mol Med. 2014 Dec; 34(6):1613-21.IJ

Abstract

Skin aging is induced through complex biological processes in human skin caused by proteolysis of collagen and elastin, two structural proteins of the dermal extracellular matrix (ECM). Collagen and elastin degradation can induce the expression of matrix metalloproteinases (MMPs), as well as that of a family of zinc-dependent endopeptidases that play critical roles in skin aging. Moreover, elastase is a metalloproteinase which acts on the degradation of elastin in skin aging, and is also involved in the inhibition or the repair of wrinkle formation. Extract of the mycelium of Tricholoma matsutake (T. matsutake), or pine mushroom, is widely distributed in Asian countries. The extract is from the natural biomaterial of the mushroom which is rich in polysaccharides, including β-glucan. This extract has shown potent bioactive antioxidant, immunomodulatory and antitumoral properties. In the present study, we investigated whether the extract of the mycelium of T. matsutake has effects on elastase activity, as well as on the expression of tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) and MMP-1 under basal conditions. Our results revealed that the extract of the mycelium of T. matsutake significantly decreased elastase activity in a dose-dependent manner and reduced the levels of MMP-1 and MMP-3. On the other hand, the expression of TIMP-1 and tropoelastin was increased in fibroblasts treated with the extract of the mycelium of T. matsutake. However, collagent expression was not affected. In addition, our results demonstrated that the extract of the mycelium of T. matsutake inhibited the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-1 expression and suppressed TPA-induced p38 activity. Therefore, the inhibitory effects of the extract of the mycelium of T. matsutake on MMP-1 induction are mediated by the inhibition of p38 in human fibroblasts. Our data suggest that the extract of the mycelium of T. matsutake may prove to be an effective biomaterial for anti-wrinkle treatment, as it can obstruct the degradation of the dermal ECM.

Authors+Show Affiliations

Major in Biomedical Science, Department of Medicine, Graduate School, Chung-Ang University, Seoul, Republic of Korea.Major in Biomedical Science, Department of Medicine, Graduate School, Chung-Ang University, Seoul, Republic of Korea.Major in Biomedical Science, Department of Medicine, Graduate School, Chung-Ang University, Seoul, Republic of Korea.Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.Major in Biomedical Science, Department of Medicine, Graduate School, Chung-Ang University, Seoul, Republic of Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25319362

Citation

Kim, So Young, et al. "Extract of the Mycelium of T. Matsutake Inhibits Elastase Activity and TPA-induced MMP-1 Expression in Human Fibroblasts." International Journal of Molecular Medicine, vol. 34, no. 6, 2014, pp. 1613-21.
Kim SY, Go KC, Song YS, et al. Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts. Int J Mol Med. 2014;34(6):1613-21.
Kim, S. Y., Go, K. C., Song, Y. S., Jeong, Y. S., Kim, E. J., & Kim, B. J. (2014). Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts. International Journal of Molecular Medicine, 34(6), 1613-21. https://doi.org/10.3892/ijmm.2014.1969
Kim SY, et al. Extract of the Mycelium of T. Matsutake Inhibits Elastase Activity and TPA-induced MMP-1 Expression in Human Fibroblasts. Int J Mol Med. 2014;34(6):1613-21. PubMed PMID: 25319362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts. AU - Kim,So Young, AU - Go,Kyung Chan, AU - Song,Yi Seop, AU - Jeong,Yeon Su, AU - Kim,Eun Joo, AU - Kim,Beom Joon, Y1 - 2014/10/14/ PY - 2014/06/02/received PY - 2014/09/24/accepted PY - 2014/10/17/entrez PY - 2014/10/17/pubmed PY - 2015/7/22/medline SP - 1613 EP - 21 JF - International journal of molecular medicine JO - Int. J. Mol. Med. VL - 34 IS - 6 N2 - Skin aging is induced through complex biological processes in human skin caused by proteolysis of collagen and elastin, two structural proteins of the dermal extracellular matrix (ECM). Collagen and elastin degradation can induce the expression of matrix metalloproteinases (MMPs), as well as that of a family of zinc-dependent endopeptidases that play critical roles in skin aging. Moreover, elastase is a metalloproteinase which acts on the degradation of elastin in skin aging, and is also involved in the inhibition or the repair of wrinkle formation. Extract of the mycelium of Tricholoma matsutake (T. matsutake), or pine mushroom, is widely distributed in Asian countries. The extract is from the natural biomaterial of the mushroom which is rich in polysaccharides, including β-glucan. This extract has shown potent bioactive antioxidant, immunomodulatory and antitumoral properties. In the present study, we investigated whether the extract of the mycelium of T. matsutake has effects on elastase activity, as well as on the expression of tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) and MMP-1 under basal conditions. Our results revealed that the extract of the mycelium of T. matsutake significantly decreased elastase activity in a dose-dependent manner and reduced the levels of MMP-1 and MMP-3. On the other hand, the expression of TIMP-1 and tropoelastin was increased in fibroblasts treated with the extract of the mycelium of T. matsutake. However, collagent expression was not affected. In addition, our results demonstrated that the extract of the mycelium of T. matsutake inhibited the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-1 expression and suppressed TPA-induced p38 activity. Therefore, the inhibitory effects of the extract of the mycelium of T. matsutake on MMP-1 induction are mediated by the inhibition of p38 in human fibroblasts. Our data suggest that the extract of the mycelium of T. matsutake may prove to be an effective biomaterial for anti-wrinkle treatment, as it can obstruct the degradation of the dermal ECM. SN - 1791-244X UR - https://www.unboundmedicine.com/medline/citation/25319362/Extract_of_the_mycelium_of_T__matsutake_inhibits_elastase_activity_and_TPA_induced_MMP_1_expression_in_human_fibroblasts_ L2 - http://www.spandidos-publications.com/ijmm/34/6/1613 DB - PRIME DP - Unbound Medicine ER -