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Factors affecting stress tolerance in recalcitrant embryonic axes from seeds of four Quercus (Fagaceae) species native to the USA or China.
Ann Bot. 2014 Dec; 114(8):1747-59.AB

Abstract

BACKGROUND AND AIMS

Quercus species are often considered 'foundation' components of several temperate and/or subtropical forest ecosystems. However, the populations of some species are declining and there is considerable urgency to develop ex situ conservation strategies. In this study, the storage physiology of seeds within Quercus was explored in order to determine factors that affect survival during cryopreservation and to provide a quantitative assessment of seed recalcitrance to support future studies of this complex trait.

METHODS

Water relations and survival of excised axes in response to water loss and cryo-exposure were compared for four Quercus species from subtropical China (Q. franchetii, Q. schottkyana) and temperate USA (Q. gambelii, Q. rubra).

KEY RESULTS

Seed tissues initially had high water contents and water potentials. Desiccation tolerance of the embryonic axis was not correlated with the post-shedding rainfall patterns where the samples originated. Instead, higher desiccation tolerance was observed in samples growing in areas with colder winters. Survival following cryo-exposure correlated with desiccation tolerance. Among species, plumule tissues were more sensitive than radicles to excision, desiccation and cryo-exposure, and this led to a higher proportion of abnormally developing embryos during recovery following stress.

CONCLUSIONS

Quercus species adapted to arid and semi-humid climates still produce recalcitrant seeds. The ability to avoid freezing rather than drought may be a more important selection factor to increase desiccation tolerance. Cryopreservation of recalcitrant germplasm from temperate species is currently feasible, whilst additional protective treatments are needed for ex situ conservation of Quercus from tropical and subtropical areas.

Authors+Show Affiliations

Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China USDA-ARS National Center for Genetic Resources Preservation, Fort Collins, CO 80524, USA.USDA-ARS National Center for Genetic Resources Preservation, Fort Collins, CO 80524, USA.Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.USDA-ARS National Center for Genetic Resources Preservation, Fort Collins, CO 80524, USA christina.walters@ars.usda.gov.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25326139

Citation

Xia, Ke, et al. "Factors Affecting Stress Tolerance in Recalcitrant Embryonic Axes From Seeds of Four Quercus (Fagaceae) Species Native to the USA or China." Annals of Botany, vol. 114, no. 8, 2014, pp. 1747-59.
Xia K, Hill LM, Li DZ, et al. Factors affecting stress tolerance in recalcitrant embryonic axes from seeds of four Quercus (Fagaceae) species native to the USA or China. Ann Bot. 2014;114(8):1747-59.
Xia, K., Hill, L. M., Li, D. Z., & Walters, C. (2014). Factors affecting stress tolerance in recalcitrant embryonic axes from seeds of four Quercus (Fagaceae) species native to the USA or China. Annals of Botany, 114(8), 1747-59. https://doi.org/10.1093/aob/mcu193
Xia K, et al. Factors Affecting Stress Tolerance in Recalcitrant Embryonic Axes From Seeds of Four Quercus (Fagaceae) Species Native to the USA or China. Ann Bot. 2014;114(8):1747-59. PubMed PMID: 25326139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Factors affecting stress tolerance in recalcitrant embryonic axes from seeds of four Quercus (Fagaceae) species native to the USA or China. AU - Xia,Ke, AU - Hill,Lisa M, AU - Li,De-Zhu, AU - Walters,Christina, Y1 - 2014/10/17/ PY - 2014/10/19/entrez PY - 2014/10/19/pubmed PY - 2015/7/15/medline KW - Cyclobalanopsis KW - Quercus franchetii KW - Quercus gambelii KW - Quercus rubra KW - Quercus schottkyana KW - Seed desiccation tolerance KW - critical moisture content KW - cryopreservation KW - embryonic axis KW - oak KW - plumule KW - recalcitrance KW - subtropical SP - 1747 EP - 59 JF - Annals of botany JO - Ann. Bot. VL - 114 IS - 8 N2 - BACKGROUND AND AIMS: Quercus species are often considered 'foundation' components of several temperate and/or subtropical forest ecosystems. However, the populations of some species are declining and there is considerable urgency to develop ex situ conservation strategies. In this study, the storage physiology of seeds within Quercus was explored in order to determine factors that affect survival during cryopreservation and to provide a quantitative assessment of seed recalcitrance to support future studies of this complex trait. METHODS: Water relations and survival of excised axes in response to water loss and cryo-exposure were compared for four Quercus species from subtropical China (Q. franchetii, Q. schottkyana) and temperate USA (Q. gambelii, Q. rubra). KEY RESULTS: Seed tissues initially had high water contents and water potentials. Desiccation tolerance of the embryonic axis was not correlated with the post-shedding rainfall patterns where the samples originated. Instead, higher desiccation tolerance was observed in samples growing in areas with colder winters. Survival following cryo-exposure correlated with desiccation tolerance. Among species, plumule tissues were more sensitive than radicles to excision, desiccation and cryo-exposure, and this led to a higher proportion of abnormally developing embryos during recovery following stress. CONCLUSIONS: Quercus species adapted to arid and semi-humid climates still produce recalcitrant seeds. The ability to avoid freezing rather than drought may be a more important selection factor to increase desiccation tolerance. Cryopreservation of recalcitrant germplasm from temperate species is currently feasible, whilst additional protective treatments are needed for ex situ conservation of Quercus from tropical and subtropical areas. SN - 1095-8290 UR - https://www.unboundmedicine.com/medline/citation/25326139/Factors_affecting_stress_tolerance_in_recalcitrant_embryonic_axes_from_seeds_of_four_Quercus__Fagaceae__species_native_to_the_USA_or_China_ L2 - https://academic.oup.com/aob/article-lookup/doi/10.1093/aob/mcu193 DB - PRIME DP - Unbound Medicine ER -