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Antidiabetic, antilipidemic, and antioxidant activities of Gouania longipetala methanol leaf extract in alloxan-induced diabetic rats.
Pharm Biol. 2015 Apr; 53(4):605-14.PB

Abstract

CONTEXT

Gouania longipetala Hemsl. (Rhamnaceae) is used in folkloric medicine for treating diabetes mellitus and its associated symptoms.

OBJECTIVE

This study evaluated the antidiabetic antilipidemic and antioxidant activities of the plant methanol leaf extract.

MATERIALS AND METHODS

Diabetes was induced in rats by intraperitoneal injection of alloxan monohydrate (160 mg/kg). Three test doses (50, 100, and 150 mg/kg) of G. longipetala extract (GLE) were administered orally and the effects were compared with glibenclamide (2 mg/kg). The effect of GLE on hyperglycemia and sub-acute study for 21 d were carried out using its effect on fasting blood sugar (FBS) level. Serum biochemistry and antioxidant activity were evaluated. Histopathological evaluation of the pancreas was also done.

RESULTS

The LD50 of G. longipetala was found to be >4000 mg/kg. The extract significantly (p < 0.0001) decreased the FBS levels of treated rats from 16.2 ± 2.03 to 6.5 ± 1.52 mM/L at 150 mg/kg within 24 h. The extract decreased FBS levels of rats by 62.0, 74.8, and 75.0% on day 21 at 50, 100, and 150 mg/kg, respectively. GLE reduced the level of malondiadehyde from 23.0 ± 1.34 to 10.3 ± 0.43 mg/dL, increased superoxide dismutase activities from 2.97 ± 0.34 to 5.80 ± 0.53 IU/L at 150 mg/kg, and improved the serum lipid profile of treated rats. GLE also caused restoration of the altered histopathological changes of the pancreas.

DISCUSSION AND CONCLUSION

Gouania longipetala demonstrated significant antidiabetic, antilipidemic, and antioxidant activities that may be due to its multiple effects involving both pancreatic and extra-pancreatic mechanisms.

Authors+Show Affiliations

Department of Veterinary Physiology, Pharmacology and Biochemistry, Michael Okpara University of Agriculture , Umudike, Abia State , Nigeria and.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25330778

Citation

Ezeja, Maxwell Ikechukwu, et al. "Antidiabetic, Antilipidemic, and Antioxidant Activities of Gouania Longipetala Methanol Leaf Extract in Alloxan-induced Diabetic Rats." Pharmaceutical Biology, vol. 53, no. 4, 2015, pp. 605-14.
Ezeja MI, Anaga AO, Asuzu IU. Antidiabetic, antilipidemic, and antioxidant activities of Gouania longipetala methanol leaf extract in alloxan-induced diabetic rats. Pharm Biol. 2015;53(4):605-14.
Ezeja, M. I., Anaga, A. O., & Asuzu, I. U. (2015). Antidiabetic, antilipidemic, and antioxidant activities of Gouania longipetala methanol leaf extract in alloxan-induced diabetic rats. Pharmaceutical Biology, 53(4), 605-14. https://doi.org/10.3109/13880209.2014.935864
Ezeja MI, Anaga AO, Asuzu IU. Antidiabetic, Antilipidemic, and Antioxidant Activities of Gouania Longipetala Methanol Leaf Extract in Alloxan-induced Diabetic Rats. Pharm Biol. 2015;53(4):605-14. PubMed PMID: 25330778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antidiabetic, antilipidemic, and antioxidant activities of Gouania longipetala methanol leaf extract in alloxan-induced diabetic rats. AU - Ezeja,Maxwell Ikechukwu, AU - Anaga,Aruh Ottah, AU - Asuzu,Isaac U, Y1 - 2014/10/21/ PY - 2014/10/22/entrez PY - 2014/10/22/pubmed PY - 2015/10/16/medline KW - Fasting blood sugar KW - glibenclamide KW - histopathology KW - hyperglycemia SP - 605 EP - 14 JF - Pharmaceutical biology JO - Pharm Biol VL - 53 IS - 4 N2 - CONTEXT: Gouania longipetala Hemsl. (Rhamnaceae) is used in folkloric medicine for treating diabetes mellitus and its associated symptoms. OBJECTIVE: This study evaluated the antidiabetic antilipidemic and antioxidant activities of the plant methanol leaf extract. MATERIALS AND METHODS: Diabetes was induced in rats by intraperitoneal injection of alloxan monohydrate (160 mg/kg). Three test doses (50, 100, and 150 mg/kg) of G. longipetala extract (GLE) were administered orally and the effects were compared with glibenclamide (2 mg/kg). The effect of GLE on hyperglycemia and sub-acute study for 21 d were carried out using its effect on fasting blood sugar (FBS) level. Serum biochemistry and antioxidant activity were evaluated. Histopathological evaluation of the pancreas was also done. RESULTS: The LD50 of G. longipetala was found to be >4000 mg/kg. The extract significantly (p < 0.0001) decreased the FBS levels of treated rats from 16.2 ± 2.03 to 6.5 ± 1.52 mM/L at 150 mg/kg within 24 h. The extract decreased FBS levels of rats by 62.0, 74.8, and 75.0% on day 21 at 50, 100, and 150 mg/kg, respectively. GLE reduced the level of malondiadehyde from 23.0 ± 1.34 to 10.3 ± 0.43 mg/dL, increased superoxide dismutase activities from 2.97 ± 0.34 to 5.80 ± 0.53 IU/L at 150 mg/kg, and improved the serum lipid profile of treated rats. GLE also caused restoration of the altered histopathological changes of the pancreas. DISCUSSION AND CONCLUSION: Gouania longipetala demonstrated significant antidiabetic, antilipidemic, and antioxidant activities that may be due to its multiple effects involving both pancreatic and extra-pancreatic mechanisms. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25330778/Antidiabetic_antilipidemic_and_antioxidant_activities_of_Gouania_longipetala_methanol_leaf_extract_in_alloxan_induced_diabetic_rats_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2014.935864 DB - PRIME DP - Unbound Medicine ER -