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Effects of benzo[a]pyrene on growth, the antioxidant system, and DNA damage in earthworms (Eisenia fetida) in 2 different soil types under laboratory conditions.
Environ Toxicol Chem. 2015 Feb; 34(2):283-90.ET

Abstract

The aims of the present study were to compare the toxic effects of benzo[a]pyrene (BaP) and to screen for rapid and sensitive biomarkers that can be used to assess the environmental risks of BaP in earthworms in different natural soil types. The authors exposed Eisenia fetida to 2 types of soil (red soil and fluvo-aquic soil) spiked with different concentrations (0 mg kg(-1), 1 mg kg(-1), 10 mg kg(-1), 100 mg kg(-1), and 500 mg kg(-1)) of BaP for 7 d or 14 d. Benzo[a]pyrene-induced weight variation altered the activities of antioxidant enzymes (superoxide dismutase [SOD]; catalase [CAT]; and guaiacol peroxidase [POD]) and changed the content of malondialdehyde (MDA). In addition, using the comet assay, the authors determined the DNA damage in earthworms. The results revealed that the comet assay was suitable for evaluating the genotoxicity of BaP in the soil, even at the lowest examined concentration. The MDA content was the least sensitive indicator of BaP toxicity. A 3-way analysis of variance (ANOVA) was used to determine whether the soil type, exposure concentration, and duration affected the BaP toxicity. The antioxidant enzyme activities and the MDA content were shown to be significantly correlated with the exposure concentration. The percentage of weight variation (p < 0.001), CAT activity (p < 0.05), and SOD activity (p < 0.01) were significantly affected by the soil type, and the POD activity (p < 0.01), CAT activity (p < 0.001), and SOD activity (p < 0.001) were significantly affected by the exposure duration. Therefore, measuring DNA damage in earthworms is a simple and efficient means of assessing BaP genotoxicity in a terrestrial environment, and the effects of the soil type and exposure time on the other parameters that were investigated in E. fetida, which were used as responsive biomarkers, should be considered.

Authors+Show Affiliations

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25331318

Citation

Duan, Xiaochen, et al. "Effects of Benzo[a]pyrene On Growth, the Antioxidant System, and DNA Damage in Earthworms (Eisenia Fetida) in 2 Different Soil Types Under Laboratory Conditions." Environmental Toxicology and Chemistry, vol. 34, no. 2, 2015, pp. 283-90.
Duan X, Xu L, Song J, et al. Effects of benzo[a]pyrene on growth, the antioxidant system, and DNA damage in earthworms (Eisenia fetida) in 2 different soil types under laboratory conditions. Environ Toxicol Chem. 2015;34(2):283-90.
Duan, X., Xu, L., Song, J., Jiao, J., Liu, M., Hu, F., & Li, H. (2015). Effects of benzo[a]pyrene on growth, the antioxidant system, and DNA damage in earthworms (Eisenia fetida) in 2 different soil types under laboratory conditions. Environmental Toxicology and Chemistry, 34(2), 283-90. https://doi.org/10.1002/etc.2785
Duan X, et al. Effects of Benzo[a]pyrene On Growth, the Antioxidant System, and DNA Damage in Earthworms (Eisenia Fetida) in 2 Different Soil Types Under Laboratory Conditions. Environ Toxicol Chem. 2015;34(2):283-90. PubMed PMID: 25331318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of benzo[a]pyrene on growth, the antioxidant system, and DNA damage in earthworms (Eisenia fetida) in 2 different soil types under laboratory conditions. AU - Duan,Xiaochen, AU - Xu,Li, AU - Song,Jing, AU - Jiao,Jiaguo, AU - Liu,Manqiang, AU - Hu,Feng, AU - Li,Huixin, Y1 - 2015/01/06/ PY - 2014/07/11/received PY - 2014/08/14/revised PY - 2014/10/17/accepted PY - 2014/10/22/entrez PY - 2014/10/22/pubmed PY - 2015/5/30/medline KW - Antioxidant enzymes KW - Benzo[a]pyrene KW - Comet assay KW - Eisenia fetida KW - MDA SP - 283 EP - 90 JF - Environmental toxicology and chemistry JO - Environ Toxicol Chem VL - 34 IS - 2 N2 - The aims of the present study were to compare the toxic effects of benzo[a]pyrene (BaP) and to screen for rapid and sensitive biomarkers that can be used to assess the environmental risks of BaP in earthworms in different natural soil types. The authors exposed Eisenia fetida to 2 types of soil (red soil and fluvo-aquic soil) spiked with different concentrations (0 mg kg(-1), 1 mg kg(-1), 10 mg kg(-1), 100 mg kg(-1), and 500 mg kg(-1)) of BaP for 7 d or 14 d. Benzo[a]pyrene-induced weight variation altered the activities of antioxidant enzymes (superoxide dismutase [SOD]; catalase [CAT]; and guaiacol peroxidase [POD]) and changed the content of malondialdehyde (MDA). In addition, using the comet assay, the authors determined the DNA damage in earthworms. The results revealed that the comet assay was suitable for evaluating the genotoxicity of BaP in the soil, even at the lowest examined concentration. The MDA content was the least sensitive indicator of BaP toxicity. A 3-way analysis of variance (ANOVA) was used to determine whether the soil type, exposure concentration, and duration affected the BaP toxicity. The antioxidant enzyme activities and the MDA content were shown to be significantly correlated with the exposure concentration. The percentage of weight variation (p < 0.001), CAT activity (p < 0.05), and SOD activity (p < 0.01) were significantly affected by the soil type, and the POD activity (p < 0.01), CAT activity (p < 0.001), and SOD activity (p < 0.001) were significantly affected by the exposure duration. Therefore, measuring DNA damage in earthworms is a simple and efficient means of assessing BaP genotoxicity in a terrestrial environment, and the effects of the soil type and exposure time on the other parameters that were investigated in E. fetida, which were used as responsive biomarkers, should be considered. SN - 1552-8618 UR - https://www.unboundmedicine.com/medline/citation/25331318/Effects_of_benzo[a]pyrene_on_growth_the_antioxidant_system_and_DNA_damage_in_earthworms__Eisenia_fetida__in_2_different_soil_types_under_laboratory_conditions_ L2 - https://doi.org/10.1002/etc.2785 DB - PRIME DP - Unbound Medicine ER -