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[Serumimmunological study of moxibustion on helicobacter pylori gastritis in rats].
Zhongguo Zhen Jiu. 2014 Aug; 34(8):783-90.ZZ

Abstract

OBJECTIVE

To explore the immune mechanism of moxibustion on protecting gastric mucosa injury.

METHODS

Forty healthy SD rats were randomly divided into four groups: a blank group, a model group, a moxibustion acupoint group and a moxibustion non-acupoint group, 10 rats in each one. Eight days before model establishment, moxibustion at "Zusanli" (ST 36), "Zhongwan" (CV 12), "Guanyuan" (CV 4), "Pishu" (BL 20) and "Weishu" (BL 21) was applied in the moxibustion acupoint group while these acupoints' controlled points were selected in the moxibustion non-acupoint group, and no treatment was given in the model group, once a day in three groups for continuous 16 days. The helicobacter pylori (Hp) model was established by intragastric administration of Hp. HE staining microscopic examination was used to observe inflammation severity in gastric mucosa, and enzyme-linked immunosorbent assay (ELISA) was adapted to measure content of heat shock protein (HSP) 72, TNF-alpha and IL-1beta, and real-time quantitative PCR was used to measure the expression of TLR2 mRNA, TLR4 mRNA, CD14 mRNA and MyD88 mRNA in peripheral blood mononuclear cells, and western blot method was used to measure content of NFkappaB and IkappaBalpha in peripheral blood mononuclear cells.

RESULTS

Compared with the blank group, the expression of HP could be seen in the smear of gastric mucosa by Gram's staining in the model group; the inflammation severity score was obviously increased as well as content of serum HSP 72 and TNF-alpha and IL-1beta in gastric tissue; and expression of TLR2, 4 mRNA, CD14 mRNA, MyD88 mRNA, NFkappaB was increased (P < 0.01), but the expression of IkappaBalpha was reduced (P < 0.05). After the moxibustion, the inflammation severity score was reduced in the moxibustion acupoint group, and the content of serum HSP 72 was increased, and the expression of TNF-alpha and IL-1beta in gastric tissue and expression of TLR2 mRNA, TLR4 mRNA, CD14 mRNA, MyD88 mRNA and NFkappaB were reduced (P < 0.01), but the expression of IkappaBalpha was increased (P < 0.05). The differences between the moxibustion non-acupoint group and the model group were not significant (P > 0.05).

CONCLUSION

The pretreatment of moxibustion at acupoints could induce the over expression of serum HSP 72. By combining TLR 2 and 4 receptors to trigger receptor signal transduction pathways, the releases of downstream signal substances are regulated; as a result, the releases of related immune substances are regulated to relieve the gastric mucosa injury of rats with HP gastritis.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

25335260

Citation

Peng, Yan, et al. "[Serumimmunological Study of Moxibustion On Helicobacter Pylori Gastritis in Rats]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 34, no. 8, 2014, pp. 783-90.
Peng Y, Yi SX, Feng YS, et al. [Serumimmunological study of moxibustion on helicobacter pylori gastritis in rats]. Zhongguo Zhen Jiu. 2014;34(8):783-90.
Peng, Y., Yi, S. X., Feng, Y. S., Shi, D. M., Hou, Y. L., & Lin, Y. P. (2014). [Serumimmunological study of moxibustion on helicobacter pylori gastritis in rats]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 34(8), 783-90.
Peng Y, et al. [Serumimmunological Study of Moxibustion On Helicobacter Pylori Gastritis in Rats]. Zhongguo Zhen Jiu. 2014;34(8):783-90. PubMed PMID: 25335260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Serumimmunological study of moxibustion on helicobacter pylori gastritis in rats]. AU - Peng,Yan, AU - Yi,Shou-Xiang, AU - Feng,Ying-Shuai, AU - Shi,Dong-Mei, AU - Hou,Yan-Ling, AU - Lin,Ya-Ping, PY - 2014/10/23/entrez PY - 2014/10/23/pubmed PY - 2014/11/14/medline SP - 783 EP - 90 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 34 IS - 8 N2 - OBJECTIVE: To explore the immune mechanism of moxibustion on protecting gastric mucosa injury. METHODS: Forty healthy SD rats were randomly divided into four groups: a blank group, a model group, a moxibustion acupoint group and a moxibustion non-acupoint group, 10 rats in each one. Eight days before model establishment, moxibustion at "Zusanli" (ST 36), "Zhongwan" (CV 12), "Guanyuan" (CV 4), "Pishu" (BL 20) and "Weishu" (BL 21) was applied in the moxibustion acupoint group while these acupoints' controlled points were selected in the moxibustion non-acupoint group, and no treatment was given in the model group, once a day in three groups for continuous 16 days. The helicobacter pylori (Hp) model was established by intragastric administration of Hp. HE staining microscopic examination was used to observe inflammation severity in gastric mucosa, and enzyme-linked immunosorbent assay (ELISA) was adapted to measure content of heat shock protein (HSP) 72, TNF-alpha and IL-1beta, and real-time quantitative PCR was used to measure the expression of TLR2 mRNA, TLR4 mRNA, CD14 mRNA and MyD88 mRNA in peripheral blood mononuclear cells, and western blot method was used to measure content of NFkappaB and IkappaBalpha in peripheral blood mononuclear cells. RESULTS: Compared with the blank group, the expression of HP could be seen in the smear of gastric mucosa by Gram's staining in the model group; the inflammation severity score was obviously increased as well as content of serum HSP 72 and TNF-alpha and IL-1beta in gastric tissue; and expression of TLR2, 4 mRNA, CD14 mRNA, MyD88 mRNA, NFkappaB was increased (P < 0.01), but the expression of IkappaBalpha was reduced (P < 0.05). After the moxibustion, the inflammation severity score was reduced in the moxibustion acupoint group, and the content of serum HSP 72 was increased, and the expression of TNF-alpha and IL-1beta in gastric tissue and expression of TLR2 mRNA, TLR4 mRNA, CD14 mRNA, MyD88 mRNA and NFkappaB were reduced (P < 0.01), but the expression of IkappaBalpha was increased (P < 0.05). The differences between the moxibustion non-acupoint group and the model group were not significant (P > 0.05). CONCLUSION: The pretreatment of moxibustion at acupoints could induce the over expression of serum HSP 72. By combining TLR 2 and 4 receptors to trigger receptor signal transduction pathways, the releases of downstream signal substances are regulated; as a result, the releases of related immune substances are regulated to relieve the gastric mucosa injury of rats with HP gastritis. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/25335260/[Serumimmunological_study_of_moxibustion_on_helicobacter_pylori_gastritis_in_rats]_ DB - PRIME DP - Unbound Medicine ER -