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High expression of long non-coding RNA SPRY4-IT1 predicts poor prognosis of clear cell renal cell carcinoma.
Int J Clin Exp Pathol 2014; 7(9):5801-9IJ

Abstract

INTRODUCTION

Long non-coding RNAs (lncRNAs) play a key role in cellular processes, such as cell growth, apoptosis, and carcinogenesis. lncRNAs SPRY4-IT1 has recently been identified to be involved in tumorigenesis of several cancers such as non-small cell lung cancer and esophageal squamous cell carcinoma. However, the role of SPRY4-IT1 in clear cell renal cell carcinoma (ccRCC) remains unclear.

METHODS

The expression of SPRY4-IT1 was examined in ccRCC patients and renal cancer cell lines by using quantitative real-time PCR (qRT-PCR). The relationship between SPRY4-IT1 level and clinicopathological parameters of ccRCC was analyzed with the Kaplan-Meier method and Cox proportional hazards model. Small interfering RNA (siRNA) was used to suppress SPRY4-IT1 expression in renal cancer cell line 786-O. In vitro assays were performed to further explore its role in renal cancer progressio.

RESULTS

The relative level of SPRY4-IT1 was significantly higher in ccRCC tissues compared to the adjacent normal renal tissues. And higher expression of SPRY4-IT1 was found in renal cancer cell lines compared with the normal human proximal tubule epithelial cell line HK-2. The ccRCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. Multivariate analyses by Cox's proportional hazard model revealed that expression of SPRY4-IT1 was an independent prognostic factor in ccRCC. In vitro assays, our results indicated that knockdown of SPRY4-IT1 reduced renal cancer cell proliferation, migration, and invasion.

CONCLUSIONS

Our data suggested that lncRNA SPRY4-IT1 might be considered as a potential prognostic indicator and a potential target for therapeutic intervention in RC.

Authors+Show Affiliations

Department of Urology, Shanghai Tenth People's Hospital, Tongji University Shanghai 200072, China.Department of Urology, Shanghai Tenth People's Hospital, Tongji University Shanghai 200072, China.Department of Urology, Shanghai Tenth People's Hospital, Tongji University Shanghai 200072, China.Department of Urology, Shanghai Tenth People's Hospital, Tongji University Shanghai 200072, China.Department of Urology, Shanghai Tenth People's Hospital, Tongji University Shanghai 200072, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25337221

Citation

Zhang, Hai-Min, et al. "High Expression of Long Non-coding RNA SPRY4-IT1 Predicts Poor Prognosis of Clear Cell Renal Cell Carcinoma." International Journal of Clinical and Experimental Pathology, vol. 7, no. 9, 2014, pp. 5801-9.
Zhang HM, Yang FQ, Yan Y, et al. High expression of long non-coding RNA SPRY4-IT1 predicts poor prognosis of clear cell renal cell carcinoma. Int J Clin Exp Pathol. 2014;7(9):5801-9.
Zhang, H. M., Yang, F. Q., Yan, Y., Che, J. P., & Zheng, J. H. (2014). High expression of long non-coding RNA SPRY4-IT1 predicts poor prognosis of clear cell renal cell carcinoma. International Journal of Clinical and Experimental Pathology, 7(9), pp. 5801-9.
Zhang HM, et al. High Expression of Long Non-coding RNA SPRY4-IT1 Predicts Poor Prognosis of Clear Cell Renal Cell Carcinoma. Int J Clin Exp Pathol. 2014;7(9):5801-9. PubMed PMID: 25337221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High expression of long non-coding RNA SPRY4-IT1 predicts poor prognosis of clear cell renal cell carcinoma. AU - Zhang,Hai-Min, AU - Yang,Feng-Qiang, AU - Yan,Yang, AU - Che,Jian-Ping, AU - Zheng,Jun-Hua, Y1 - 2014/08/15/ PY - 2014/07/02/received PY - 2014/08/20/accepted PY - 2014/10/23/entrez PY - 2014/10/23/pubmed PY - 2015/7/3/medline KW - Long non-coding RNAs KW - SPRY4-IT1 KW - clear cell renal cell carcinoma KW - prognosis SP - 5801 EP - 9 JF - International journal of clinical and experimental pathology JO - Int J Clin Exp Pathol VL - 7 IS - 9 N2 - INTRODUCTION: Long non-coding RNAs (lncRNAs) play a key role in cellular processes, such as cell growth, apoptosis, and carcinogenesis. lncRNAs SPRY4-IT1 has recently been identified to be involved in tumorigenesis of several cancers such as non-small cell lung cancer and esophageal squamous cell carcinoma. However, the role of SPRY4-IT1 in clear cell renal cell carcinoma (ccRCC) remains unclear. METHODS: The expression of SPRY4-IT1 was examined in ccRCC patients and renal cancer cell lines by using quantitative real-time PCR (qRT-PCR). The relationship between SPRY4-IT1 level and clinicopathological parameters of ccRCC was analyzed with the Kaplan-Meier method and Cox proportional hazards model. Small interfering RNA (siRNA) was used to suppress SPRY4-IT1 expression in renal cancer cell line 786-O. In vitro assays were performed to further explore its role in renal cancer progressio. RESULTS: The relative level of SPRY4-IT1 was significantly higher in ccRCC tissues compared to the adjacent normal renal tissues. And higher expression of SPRY4-IT1 was found in renal cancer cell lines compared with the normal human proximal tubule epithelial cell line HK-2. The ccRCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. Multivariate analyses by Cox's proportional hazard model revealed that expression of SPRY4-IT1 was an independent prognostic factor in ccRCC. In vitro assays, our results indicated that knockdown of SPRY4-IT1 reduced renal cancer cell proliferation, migration, and invasion. CONCLUSIONS: Our data suggested that lncRNA SPRY4-IT1 might be considered as a potential prognostic indicator and a potential target for therapeutic intervention in RC. SN - 1936-2625 UR - https://www.unboundmedicine.com/medline/citation/25337221/High_expression_of_long_non_coding_RNA_SPRY4_IT1_predicts_poor_prognosis_of_clear_cell_renal_cell_carcinoma_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25337221/ DB - PRIME DP - Unbound Medicine ER -