TY - JOUR T1 - Identification and quantification of cannabinoids in Cannabis sativa L. plants by high performance liquid chromatography-mass spectrometry. AU - Aizpurua-Olaizola,Oier, AU - Omar,Jone, AU - Navarro,Patricia, AU - Olivares,Maitane, AU - Etxebarria,Nestor, AU - Usobiaga,Aresatz, Y1 - 2014/10/23/ PY - 2014/07/24/received PY - 2014/09/09/accepted PY - 2014/09/04/revised PY - 2014/10/24/entrez PY - 2014/10/24/pubmed PY - 2015/7/2/medline SP - 7549 EP - 60 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 406 IS - 29 N2 - High performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) has been successfully applied to cannabis plant extracts in order to identify cannabinoid compounds after their quantitative isolation by means of supercritical fluid extraction (SFE). MS conditions were optimized by means of a central composite design (CCD) approach, and the analysis method was fully validated. Six major cannabinoids [tetrahydrocannabinolic acid (THCA), tetrahydrocannabinol (THC), cannabidiol (CBD), tetrahydrocannabivarin (THCV), cannabigerol (CBG), and cannabinol (CBN)] were quantified (RSD < 10%), and seven more cannabinoids were identified and verified by means of a liquid chromatograph coupled to a quadrupole-time-of-flight (Q-ToF) detector. Finally, based on the distribution of the analyzed cannabinoids in 30 Cannabis sativa L. plant varieties and the principal component analysis (PCA) of the resulting data, a clear difference was observed between outdoor and indoor grown plants, which was attributed to a higher concentration of THC, CBN, and CBD in outdoor grown plants. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/25338935/Identification_and_quantification_of_cannabinoids_in_Cannabis_sativa_L__plants_by_high_performance_liquid_chromatography_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -