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Diverse formulas for spider dragline fibers demonstrated by molecular and mechanical characterization of spitting spider silk.
Biomacromolecules 2014; 15(12):4598-605B

Abstract

Spider silks have outstanding mechanical properties. Most research has focused on dragline silk proteins (major ampullate spidroins, MaSps) from orb-weaving spiders. Using silk gland expression libraries from the haplogyne spider Scytodes thoracica, we discovered two novel spidroins (S. thoracica fibroin 1 and 2). The amino acid composition of S. thoracica silk glands and dragline fibers suggest that fibroin 1 is the major component of S. thoracica dragline silk. Fibroin 1 is dominated by glycine-alanine motifs, and lacks sequence motifs associated with orb-weaver MaSps. We hypothesize fibroin 2 is a piriform or aciniform silk protein, based on amino acid composition, spigot morphology, and phylogenetic analyses. S. thoracica's dragline silk is less tough than previously reported, but is still comparable to other dragline silks. Our analyses suggest that dragline silk proteins evolved multiple times. This demonstrates that spider dragline silk is more diverse than previously understood, providing alternative high performance silk designs.

Authors+Show Affiliations

Department of Biological Sciences, University of Massachusetts-Lowell , Lowell, Massachusetts 01854, United States.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25340514

Citation

Correa-Garhwal, Sandra M., and Jessica E. Garb. "Diverse Formulas for Spider Dragline Fibers Demonstrated By Molecular and Mechanical Characterization of Spitting Spider Silk." Biomacromolecules, vol. 15, no. 12, 2014, pp. 4598-605.
Correa-Garhwal SM, Garb JE. Diverse formulas for spider dragline fibers demonstrated by molecular and mechanical characterization of spitting spider silk. Biomacromolecules. 2014;15(12):4598-605.
Correa-Garhwal, S. M., & Garb, J. E. (2014). Diverse formulas for spider dragline fibers demonstrated by molecular and mechanical characterization of spitting spider silk. Biomacromolecules, 15(12), pp. 4598-605. doi:10.1021/bm501409n.
Correa-Garhwal SM, Garb JE. Diverse Formulas for Spider Dragline Fibers Demonstrated By Molecular and Mechanical Characterization of Spitting Spider Silk. Biomacromolecules. 2014 Dec 8;15(12):4598-605. PubMed PMID: 25340514.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diverse formulas for spider dragline fibers demonstrated by molecular and mechanical characterization of spitting spider silk. AU - Correa-Garhwal,Sandra M, AU - Garb,Jessica E, Y1 - 2014/11/07/ PY - 2014/10/24/entrez PY - 2014/10/24/pubmed PY - 2015/9/1/medline SP - 4598 EP - 605 JF - Biomacromolecules JO - Biomacromolecules VL - 15 IS - 12 N2 - Spider silks have outstanding mechanical properties. Most research has focused on dragline silk proteins (major ampullate spidroins, MaSps) from orb-weaving spiders. Using silk gland expression libraries from the haplogyne spider Scytodes thoracica, we discovered two novel spidroins (S. thoracica fibroin 1 and 2). The amino acid composition of S. thoracica silk glands and dragline fibers suggest that fibroin 1 is the major component of S. thoracica dragline silk. Fibroin 1 is dominated by glycine-alanine motifs, and lacks sequence motifs associated with orb-weaver MaSps. We hypothesize fibroin 2 is a piriform or aciniform silk protein, based on amino acid composition, spigot morphology, and phylogenetic analyses. S. thoracica's dragline silk is less tough than previously reported, but is still comparable to other dragline silks. Our analyses suggest that dragline silk proteins evolved multiple times. This demonstrates that spider dragline silk is more diverse than previously understood, providing alternative high performance silk designs. SN - 1526-4602 UR - https://www.unboundmedicine.com/medline/citation/25340514/Diverse_formulas_for_spider_dragline_fibers_demonstrated_by_molecular_and_mechanical_characterization_of_spitting_spider_silk_ L2 - https://dx.doi.org/10.1021/bm501409n DB - PRIME DP - Unbound Medicine ER -