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Multifunctional amaranth cystatin inhibits endogenous and digestive insect cysteine endopeptidases: A potential tool to prevent proteolysis and for the control of insect pests.
Biotechnol Appl Biochem. 2015 Sep-Oct; 62(5):634-41.BA

Abstract

In a previous study, the amaranth cystatin was characterized. This cystatin is believed to provide protection from abiotic stress because its transcription is induced in response to heat, drought, and salinity. It has also been shown that recombinant amaranth cystatin inhibits bromelain, ficin, and cysteine endopeptidases from fungal sources and also inhibits the growth of phytopathogenic fungi. In the present study, evidence is presented regarding the potential function of amaranth cystatin as a regulator of endogenous proteinases and insect digestive proteinases. During amaranth germination and seedling growth, different proteolytic profiles were observed at different pH levels in gelatin-containing SDS-PAGE. Most of the proteolytic enzymes detected at pH 4.5 were mainly inhibited by trans-epoxysuccinyl-leucyl amido(4-guanidino)butane (E-64) and the purified recombinant amaranth cystatin. Furthermore, the recombinant amaranth cystatin was active against insect proteinases. In particular, the E-64-sensitive proteolytic digestive enzymes from Callosobruchus maculatus, Zabrotes subfasciatus, and Acanthoscelides obtectus were inhibited by the amaranth cystatin. Taken together, these results suggest multiple roles for cystatin in amaranth, specifically during germination and seedling growth and in the protection of A. hypochondriacus against insect predation. Amaranth cystatin represents a promising tool for diverse applications in the control of insect pest and for preventing undesirable proteolytic activity.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Valdés-Rodríguez S
Cinvestav-Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato León, Irapuato, Guanajuato, México.
Galván-Ramírez JP
Cinvestav-Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato León, Irapuato, Guanajuato, México.
Guerrero-Rangel A
Cinvestav-Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato León, Irapuato, Guanajuato, México.
Cedro-Tanda A
Cinvestav-Unidad Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato León, Irapuato, Guanajuato, México.

MeSH

AmaranthusAnimalsColeopteraCystatinsCysteine EndopeptidasesCysteine Proteinase InhibitorsGerminationInsect ControlInsectaLarvaProteolysisSeedlingsSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25345487

Citation

Valdés-Rodríguez, Silvia, et al. "Multifunctional Amaranth Cystatin Inhibits Endogenous and Digestive Insect Cysteine Endopeptidases: a Potential Tool to Prevent Proteolysis and for the Control of Insect Pests." Biotechnology and Applied Biochemistry, vol. 62, no. 5, 2015, pp. 634-41.
Valdés-Rodríguez S, Galván-Ramírez JP, Guerrero-Rangel A, et al. Multifunctional amaranth cystatin inhibits endogenous and digestive insect cysteine endopeptidases: A potential tool to prevent proteolysis and for the control of insect pests. Biotechnol Appl Biochem. 2015;62(5):634-41.
Valdés-Rodríguez, S., Galván-Ramírez, J. P., Guerrero-Rangel, A., & Cedro-Tanda, A. (2015). Multifunctional amaranth cystatin inhibits endogenous and digestive insect cysteine endopeptidases: A potential tool to prevent proteolysis and for the control of insect pests. Biotechnology and Applied Biochemistry, 62(5), 634-41. https://doi.org/10.1002/bab.1313
Valdés-Rodríguez S, et al. Multifunctional Amaranth Cystatin Inhibits Endogenous and Digestive Insect Cysteine Endopeptidases: a Potential Tool to Prevent Proteolysis and for the Control of Insect Pests. Biotechnol Appl Biochem. 2015 Sep-Oct;62(5):634-41. PubMed PMID: 25345487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multifunctional amaranth cystatin inhibits endogenous and digestive insect cysteine endopeptidases: A potential tool to prevent proteolysis and for the control of insect pests. AU - Valdés-Rodríguez,Silvia, AU - Galván-Ramírez,Juan Pablo, AU - Guerrero-Rangel,Armando, AU - Cedro-Tanda,Alberto, Y1 - 2015/02/06/ PY - 2014/06/30/received PY - 2014/10/17/accepted PY - 2014/10/28/entrez PY - 2014/10/28/pubmed PY - 2016/7/28/medline KW - Amaranthus hypochondriacus L KW - cysteine protease inhibitor KW - digestive insect enzymes KW - seed germination SP - 634 EP - 41 JF - Biotechnology and applied biochemistry JO - Biotechnol Appl Biochem VL - 62 IS - 5 N2 - In a previous study, the amaranth cystatin was characterized. This cystatin is believed to provide protection from abiotic stress because its transcription is induced in response to heat, drought, and salinity. It has also been shown that recombinant amaranth cystatin inhibits bromelain, ficin, and cysteine endopeptidases from fungal sources and also inhibits the growth of phytopathogenic fungi. In the present study, evidence is presented regarding the potential function of amaranth cystatin as a regulator of endogenous proteinases and insect digestive proteinases. During amaranth germination and seedling growth, different proteolytic profiles were observed at different pH levels in gelatin-containing SDS-PAGE. Most of the proteolytic enzymes detected at pH 4.5 were mainly inhibited by trans-epoxysuccinyl-leucyl amido(4-guanidino)butane (E-64) and the purified recombinant amaranth cystatin. Furthermore, the recombinant amaranth cystatin was active against insect proteinases. In particular, the E-64-sensitive proteolytic digestive enzymes from Callosobruchus maculatus, Zabrotes subfasciatus, and Acanthoscelides obtectus were inhibited by the amaranth cystatin. Taken together, these results suggest multiple roles for cystatin in amaranth, specifically during germination and seedling growth and in the protection of A. hypochondriacus against insect predation. Amaranth cystatin represents a promising tool for diverse applications in the control of insect pest and for preventing undesirable proteolytic activity. SN - 1470-8744 UR - https://www.unboundmedicine.com/medline/citation/25345487/Multifunctional_amaranth_cystatin_inhibits_endogenous_and_digestive_insect_cysteine_endopeptidases:_A_potential_tool_to_prevent_proteolysis_and_for_the_control_of_insect_pests_ DB - PRIME DP - Unbound Medicine ER -