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[Multiplication of mycobacterium leprae in nude mice].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Oct; 11(5):358-62.ZY

Abstract

This article reported the multiplication of mycobacterium leprae in immune-deficient animals-nude mice (NIH/nu/nu). Mycobacterium leprae taken from multibacillary leprosy patient were inoculated with 1.0 x 10(4)/foot pad into right hind foot pad(FP) of nude mice and CFW mice. The results of bacterial enumeration showed that acid-fast bacillus (AFB) in the nude mice continuously multiplied. At 21 months after inoculation, the numbers of AFB increased by over 108,000-folds and reached greater than 1.08 x 10(9)/FP (greater than 5.4 x 10(10)/g tissues), while the CFW mice developed only 3.68 x 10(6)/FP and exhibited their characteristic limited growth pattern. It is indicated that the phase of logarithmic growth was prolonged in nude mice. The AFB multiplied were identified as mycobacterium leprae by following identification test: (1) failure to growth on artificial media; (2) loss of acid-fastness by pyridine extraction; (3) typical growth curve of mycobacterium leprae in foot-pad and sensitivity to antileprosy drugs. The histopathological examination of nude mice foot-pad inoculated were found lepromatous lesions, in which enormous numbers of AFB were seen on 476 days postinoculation, AFB disseminated into the fear, nose, sciatic nerve, testicle, lymphnodes, liver, spleen, lung, and noninoculated foot-pad. As mentioned above, it is considered that experimental leprosy was established in nude mice.

Authors

No affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

2534616

Citation

Wang, H Y.. "[Multiplication of Mycobacterium Leprae in Nude Mice]." Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, vol. 11, no. 5, 1989, pp. 358-62.
Wang HY. [Multiplication of mycobacterium leprae in nude mice]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989;11(5):358-62.
Wang, H. Y. (1989). [Multiplication of mycobacterium leprae in nude mice]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, 11(5), 358-62.
Wang HY. [Multiplication of Mycobacterium Leprae in Nude Mice]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989;11(5):358-62. PubMed PMID: 2534616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Multiplication of mycobacterium leprae in nude mice]. A1 - Wang,H Y, PY - 1989/10/1/pubmed PY - 1989/10/1/medline PY - 1989/10/1/entrez SP - 358 EP - 62 JF - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae JO - Zhongguo Yi Xue Ke Xue Yuan Xue Bao VL - 11 IS - 5 N2 - This article reported the multiplication of mycobacterium leprae in immune-deficient animals-nude mice (NIH/nu/nu). Mycobacterium leprae taken from multibacillary leprosy patient were inoculated with 1.0 x 10(4)/foot pad into right hind foot pad(FP) of nude mice and CFW mice. The results of bacterial enumeration showed that acid-fast bacillus (AFB) in the nude mice continuously multiplied. At 21 months after inoculation, the numbers of AFB increased by over 108,000-folds and reached greater than 1.08 x 10(9)/FP (greater than 5.4 x 10(10)/g tissues), while the CFW mice developed only 3.68 x 10(6)/FP and exhibited their characteristic limited growth pattern. It is indicated that the phase of logarithmic growth was prolonged in nude mice. The AFB multiplied were identified as mycobacterium leprae by following identification test: (1) failure to growth on artificial media; (2) loss of acid-fastness by pyridine extraction; (3) typical growth curve of mycobacterium leprae in foot-pad and sensitivity to antileprosy drugs. The histopathological examination of nude mice foot-pad inoculated were found lepromatous lesions, in which enormous numbers of AFB were seen on 476 days postinoculation, AFB disseminated into the fear, nose, sciatic nerve, testicle, lymphnodes, liver, spleen, lung, and noninoculated foot-pad. As mentioned above, it is considered that experimental leprosy was established in nude mice. SN - 1000-503X UR - https://www.unboundmedicine.com/medline/citation/2534616/[Multiplication_of_mycobacterium_leprae_in_nude_mice]_ L2 - https://medlineplus.gov/mycobacterialinfections.html DB - PRIME DP - Unbound Medicine ER -
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