TY - JOUR T1 - Presence and impact of allelic variations of two alternative s-kdr mutations, M918T and M918L, in the voltage-gated sodium channel of the green peach aphid Myzus persicae. AU - Panini,Michela, AU - Anaclerio,Matteo, AU - Puggioni,Vincenzo, AU - Stagnati,Lorenzo, AU - Nauen,Ralf, AU - Mazzoni,Emanuele, Y1 - 2014/11/10/ PY - 2014/04/17/received PY - 2014/10/15/revised PY - 2014/10/16/accepted PY - 2014/10/28/entrez PY - 2014/10/28/pubmed PY - 2016/3/26/medline KW - M918L KW - Myzus persicae KW - kdr KW - knockdown resistance KW - pyrethroid resistance KW - target-site mutation SP - 878 EP - 84 JF - Pest management science JO - Pest Manag Sci VL - 71 IS - 6 N2 - BACKGROUND: Pyrethroids have been widely employed in order to control several agricultural pests, including Myzus persicae. Target-site resistance is the main mechanism that confers insensitivity to this class of compounds, and the most common amino acid substitutions are kdr (L1014F) and s-kdr (M918T), but recently another mutation in the s-kdr locus (M918L) has been described in French and Korean populations of M. persicae. RESULTS: Molecular analysis of several Italian populations of M. persicae by pyrosequencing revealed the presence of the new s-kdr mutation (M918L) in different forms. It was found in two different nucleotide polymorphisms (a/t or a/c substitution), in heterozygous or homozygous status, and also in combination with the classic kdr and s-kdr. Bioassays on populations carrying the M918L mutation show that it strongly affects pyrethroid efficacy, particularly of type II pyrethroids such as lambda-cyhalothrin, while it has no effect against DDT. CONCLUSION: This work provides more information about the new s-kdr M918L mutation in M. persicae, describing a more complicated situation arising from the possible combination with the classic L1014F and M918T. Our data open new questions concerning the origin of these new genotypes with different combinations of target-site mutations, and also their possible influence on control strategies. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/25346186/Presence_and_impact_of_allelic_variations_of_two_alternative_s_kdr_mutations_M918T_and_M918L_in_the_voltage_gated_sodium_channel_of_the_green_peach_aphid_Myzus_persicae_ DB - PRIME DP - Unbound Medicine ER -