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Long-term spinal ventral root reimplantation, but not bone marrow mononuclear cell treatment, positively influences ultrastructural synapse recovery and motor axonal regrowth.
Int J Mol Sci. 2014 Oct 28; 15(11):19535-51.IJ

Abstract

We recently proposed a new surgical approach to treat ventral root avulsion, resulting in motoneuron protection. The present work combined such a surgical approach with bone marrow mononuclear cells (MC) therapy. Therefore, MC were added to the site of reimplantation. Female Lewis rats (seven weeks old) were subjected to unilateral ventral root avulsion (VRA) at L4, L5 and L6 levels and divided into the following groups (n = 5 for each group): Avulsion, sealant reimplanted roots and sealant reimplanted roots plus MC. After four weeks and 12 weeks post-surgery, the lumbar intumescences were processed by transmission electron microscopy, to analyze synaptic inputs to the repaired α motoneurons. Also, the ipsi and contralateral sciatic nerves were processed for axon counting and morphometry. The ultrastructural results indicated a significant preservation of inhibitory pre-synaptic boutons in the groups repaired with sealant alone and associated with MC therapy. Moreover, the average number of axons was higher in treated groups when compared to avulsion only. Complementary to the fiber counting, the morphometric analysis of axonal diameter and "g" ratio demonstrated that root reimplantation improved the motor component recovery. In conclusion, the data herein demonstrate that root reimplantation at the lesion site may be considered a therapeutic approach, following proximal lesions in the interface of central nervous system (CNS) and peripheral nervous system (PNS), and that MC therapy does not further improve the regenerative recovery, up to 12 weeks post lesion.

Authors+Show Affiliations

Department of Structural and Functional Biology, University of Campinas (UNICAMP), PO Box 6109, Campinas 13083-970, São Paulo, Brazil. robertabarbizan@yahoo.com.br.Department of Structural and Functional Biology, University of Campinas (UNICAMP), PO Box 6109, Campinas 13083-970, São Paulo, Brazil. mateusvidigal@hotmail.com.Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu 18610-307, São Paulo, Brazil. rui.ead@gmail.com.Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu 18610-307, São Paulo, Brazil. bbviera@gnosis.com.br.Department of Structural and Functional Biology, University of Campinas (UNICAMP), PO Box 6109, Campinas 13083-970, São Paulo, Brazil. alroliv@unicamp.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25353176

Citation

Barbizan, Roberta, et al. "Long-term Spinal Ventral Root Reimplantation, but Not Bone Marrow Mononuclear Cell Treatment, Positively Influences Ultrastructural Synapse Recovery and Motor Axonal Regrowth." International Journal of Molecular Sciences, vol. 15, no. 11, 2014, pp. 19535-51.
Barbizan R, Castro MV, Ferreira RS, et al. Long-term spinal ventral root reimplantation, but not bone marrow mononuclear cell treatment, positively influences ultrastructural synapse recovery and motor axonal regrowth. Int J Mol Sci. 2014;15(11):19535-51.
Barbizan, R., Castro, M. V., Ferreira, R. S., Barraviera, B., & Oliveira, A. L. (2014). Long-term spinal ventral root reimplantation, but not bone marrow mononuclear cell treatment, positively influences ultrastructural synapse recovery and motor axonal regrowth. International Journal of Molecular Sciences, 15(11), 19535-51. https://doi.org/10.3390/ijms151119535
Barbizan R, et al. Long-term Spinal Ventral Root Reimplantation, but Not Bone Marrow Mononuclear Cell Treatment, Positively Influences Ultrastructural Synapse Recovery and Motor Axonal Regrowth. Int J Mol Sci. 2014 Oct 28;15(11):19535-51. PubMed PMID: 25353176.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term spinal ventral root reimplantation, but not bone marrow mononuclear cell treatment, positively influences ultrastructural synapse recovery and motor axonal regrowth. AU - Barbizan,Roberta, AU - Castro,Mateus V, AU - Ferreira,Rui Seabra,Jr AU - Barraviera,Benedito, AU - Oliveira,Alexandre L R, Y1 - 2014/10/28/ PY - 2014/07/17/received PY - 2014/09/27/revised PY - 2014/10/11/accepted PY - 2014/10/30/entrez PY - 2014/10/30/pubmed PY - 2015/6/26/medline SP - 19535 EP - 51 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 15 IS - 11 N2 - We recently proposed a new surgical approach to treat ventral root avulsion, resulting in motoneuron protection. The present work combined such a surgical approach with bone marrow mononuclear cells (MC) therapy. Therefore, MC were added to the site of reimplantation. Female Lewis rats (seven weeks old) were subjected to unilateral ventral root avulsion (VRA) at L4, L5 and L6 levels and divided into the following groups (n = 5 for each group): Avulsion, sealant reimplanted roots and sealant reimplanted roots plus MC. After four weeks and 12 weeks post-surgery, the lumbar intumescences were processed by transmission electron microscopy, to analyze synaptic inputs to the repaired α motoneurons. Also, the ipsi and contralateral sciatic nerves were processed for axon counting and morphometry. The ultrastructural results indicated a significant preservation of inhibitory pre-synaptic boutons in the groups repaired with sealant alone and associated with MC therapy. Moreover, the average number of axons was higher in treated groups when compared to avulsion only. Complementary to the fiber counting, the morphometric analysis of axonal diameter and "g" ratio demonstrated that root reimplantation improved the motor component recovery. In conclusion, the data herein demonstrate that root reimplantation at the lesion site may be considered a therapeutic approach, following proximal lesions in the interface of central nervous system (CNS) and peripheral nervous system (PNS), and that MC therapy does not further improve the regenerative recovery, up to 12 weeks post lesion. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/25353176/Long_term_spinal_ventral_root_reimplantation_but_not_bone_marrow_mononuclear_cell_treatment_positively_influences_ultrastructural_synapse_recovery_and_motor_axonal_regrowth_ L2 - http://www.mdpi.com/resolver?pii=ijms151119535 DB - PRIME DP - Unbound Medicine ER -