Tags

Type your tag names separated by a space and hit enter

Separation and characterization of soluble esterified and glycoside-bound phenolic compounds in dry-blanched peanut skins by liquid chromatography-electrospray ionization mass spectrometry.
J Agric Food Chem. 2014 Nov 26; 62(47):11488-504.JA

Abstract

A large variety of soluble phenolic compounds, including phenolic acids (hydroxybenzoic acids, ethyl protocatechuate, and hydroxycinnamic acids, as well as phenylacetic acid and phenyllactic acid), stilbenes (trans-piceatannol and trans-3,3',5,5'-tetrahydroxy-4'-methoxystilbene), flavan-3-ols (e.g., (-)-epicatechin, (+)-catechin, (-)-epiafzelechin, and their polymers (the proanthocyanidins, PACs)), other flavonoids (e.g., isoflavones, flavanols, and flavones), and biflavonoids, were released from esters and glycosides by base/acid hydrolysis and identified in acetonic extracts of dry-blanched peanut skins (PS). Reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with electrospray ionization mass spectrometry (ESI-MS(n)) was applied to separate and identify the phenolic constituents. Tentative identification of the separated phenolics was based on molecular ions and MS(n) fragmentation patterns acquired by ESI-MS in the negative-ion mode. Identification of free phenolic acids, stilbenes, and flavonoids was also achieved by commercial standards and by published literature data. Quantification was performed on the basis of peak areas of the UV signals from the HPLC chromatograms and calibration curves of the commercial standards. The flavonoids of PS exist mostly in glycoside-bound forms, but the aglycones can be liberated upon acid hydrolysis. PS contain significantly more PACs compared to free phenolic compounds: PAC monomers to tetramers constituted 92.0% of esterified phenolic compounds. The PAC monomer ((+)-catechin) and dimers are the main phenolics released from glycosides and account for 31.7 and 59.1%, respectively, of the total glycoside-bound phenolic compounds.

Authors+Show Affiliations

Department of Food Science and Technology, College of Agricultural and Environmental Sciences, The University of Georgia , 100 Cedar Street, Athens, Georgia 30602-2610, United States.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25354220

Citation

Ma, Yuanyuan, et al. "Separation and Characterization of Soluble Esterified and Glycoside-bound Phenolic Compounds in Dry-blanched Peanut Skins By Liquid Chromatography-electrospray Ionization Mass Spectrometry." Journal of Agricultural and Food Chemistry, vol. 62, no. 47, 2014, pp. 11488-504.
Ma Y, Kosińska-Cagnazzo A, Kerr WL, et al. Separation and characterization of soluble esterified and glycoside-bound phenolic compounds in dry-blanched peanut skins by liquid chromatography-electrospray ionization mass spectrometry. J Agric Food Chem. 2014;62(47):11488-504.
Ma, Y., Kosińska-Cagnazzo, A., Kerr, W. L., Amarowicz, R., Swanson, R. B., & Pegg, R. B. (2014). Separation and characterization of soluble esterified and glycoside-bound phenolic compounds in dry-blanched peanut skins by liquid chromatography-electrospray ionization mass spectrometry. Journal of Agricultural and Food Chemistry, 62(47), 11488-504. https://doi.org/10.1021/jf503836n
Ma Y, et al. Separation and Characterization of Soluble Esterified and Glycoside-bound Phenolic Compounds in Dry-blanched Peanut Skins By Liquid Chromatography-electrospray Ionization Mass Spectrometry. J Agric Food Chem. 2014 Nov 26;62(47):11488-504. PubMed PMID: 25354220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and characterization of soluble esterified and glycoside-bound phenolic compounds in dry-blanched peanut skins by liquid chromatography-electrospray ionization mass spectrometry. AU - Ma,Yuanyuan, AU - Kosińska-Cagnazzo,Agnieszka, AU - Kerr,William L, AU - Amarowicz,Ryszard, AU - Swanson,Ruthann B, AU - Pegg,Ronald B, Y1 - 2014/11/18/ PY - 2014/10/30/entrez PY - 2014/10/30/pubmed PY - 2015/7/4/medline KW - ESI-MSn KW - RP-HPLC KW - biflavonoids KW - peanut skins KW - proanthocyanidins (PACs) SP - 11488 EP - 504 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 62 IS - 47 N2 - A large variety of soluble phenolic compounds, including phenolic acids (hydroxybenzoic acids, ethyl protocatechuate, and hydroxycinnamic acids, as well as phenylacetic acid and phenyllactic acid), stilbenes (trans-piceatannol and trans-3,3',5,5'-tetrahydroxy-4'-methoxystilbene), flavan-3-ols (e.g., (-)-epicatechin, (+)-catechin, (-)-epiafzelechin, and their polymers (the proanthocyanidins, PACs)), other flavonoids (e.g., isoflavones, flavanols, and flavones), and biflavonoids, were released from esters and glycosides by base/acid hydrolysis and identified in acetonic extracts of dry-blanched peanut skins (PS). Reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with electrospray ionization mass spectrometry (ESI-MS(n)) was applied to separate and identify the phenolic constituents. Tentative identification of the separated phenolics was based on molecular ions and MS(n) fragmentation patterns acquired by ESI-MS in the negative-ion mode. Identification of free phenolic acids, stilbenes, and flavonoids was also achieved by commercial standards and by published literature data. Quantification was performed on the basis of peak areas of the UV signals from the HPLC chromatograms and calibration curves of the commercial standards. The flavonoids of PS exist mostly in glycoside-bound forms, but the aglycones can be liberated upon acid hydrolysis. PS contain significantly more PACs compared to free phenolic compounds: PAC monomers to tetramers constituted 92.0% of esterified phenolic compounds. The PAC monomer ((+)-catechin) and dimers are the main phenolics released from glycosides and account for 31.7 and 59.1%, respectively, of the total glycoside-bound phenolic compounds. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/25354220/Separation_and_characterization_of_soluble_esterified_and_glycoside_bound_phenolic_compounds_in_dry_blanched_peanut_skins_by_liquid_chromatography_electrospray_ionization_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -