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Detection of 11 beta-hydroxysteroid dehydrogenase type 1, the glucocorticoid and mineralocorticoid receptor in various adipose tissue depots of dairy cows supplemented with conjugated linoleic acids.
J Anim Physiol Anim Nutr (Berl). 2015 Oct; 99(5):950-61.JA

Abstract

Early lactating cows mobilize adipose tissue (AT) to provide energy for milk yield and maintenance and are susceptible to metabolic disorders and impaired immune response. Conjugated linoleic acids (CLA), mainly the trans-10, cis-12 isomer, reduce milk fat synthesis and may attenuate negative energy balance. Circulating glucocorticoids (GC) are increased during parturition in dairy cows and mediate differentiating and anti-inflammatory effects via glucocorticoid (GR) and mineralocorticoid receptors (MR) in the presence of the enzyme 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1). Activated GC are the main ligands for both receptors in AT; therefore, we hypothesized that tissue-specific GC metabolism is effected by varying amounts of GR, MR and 11βHSD1 and/or their localization within AT depots. Furthermore, the lipolytic and antilipogenic effects of CLA might influence the GC/GR/MR system in AT. Therefore, we aimed to localize GR and MR as well as the expression pattern and activity of 11βHSD1 in different AT depots during early lactation in dairy cows and to identify potential effects of CLA. Primiparous German Holstein cows were divided into a control (CON) and a CLA group. From day 1 post-partum (p.p.) until sample collection, the CLA group was fed with 100 g/d CLA (contains 10 g each of the cis-9, trans-11 and the trans-10, cis-12-CLA isomers). CON cows (n = 5 each) were slaughtered on day 1, 42 and 105 p.p., while CLA cows (n = 5 each) were slaughtered on day 42 and 105 p.p. Subcutaneous fat from tailhead, withers and sternum, and visceral fat from omental, mesenteric and retroperitoneal depots were sampled. The localization of GR and 11βHSD1 in mature adipocytes - being already differentiated - indicates that GC promote other effects via GR than differentiation. Moreover, MR were observed in the stromal vascular cell fraction and positively related to the pre-adipocyte marker Pref-1. However, only marginal CLA effects were observed in this study.

Authors+Show Affiliations

Physiology and Hygiene Group, Institute of Animal Science, University of Bonn, Bonn, Germany.Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Braunschweig, Germany.Physiology and Hygiene Group, Institute of Animal Science, University of Bonn, Bonn, Germany.Physiology and Hygiene Group, Institute of Animal Science, University of Bonn, Bonn, Germany.Physiology and Hygiene Group, Institute of Animal Science, University of Bonn, Bonn, Germany.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25354306

Citation

Friedauer, K, et al. "Detection of 11 Beta-hydroxysteroid Dehydrogenase Type 1, the Glucocorticoid and Mineralocorticoid Receptor in Various Adipose Tissue Depots of Dairy Cows Supplemented With Conjugated Linoleic Acids." Journal of Animal Physiology and Animal Nutrition, vol. 99, no. 5, 2015, pp. 950-61.
Friedauer K, Dänicke S, Schulz K, et al. Detection of 11 beta-hydroxysteroid dehydrogenase type 1, the glucocorticoid and mineralocorticoid receptor in various adipose tissue depots of dairy cows supplemented with conjugated linoleic acids. J Anim Physiol Anim Nutr (Berl). 2015;99(5):950-61.
Friedauer, K., Dänicke, S., Schulz, K., Sauerwein, H., & Häussler, S. (2015). Detection of 11 beta-hydroxysteroid dehydrogenase type 1, the glucocorticoid and mineralocorticoid receptor in various adipose tissue depots of dairy cows supplemented with conjugated linoleic acids. Journal of Animal Physiology and Animal Nutrition, 99(5), 950-61. https://doi.org/10.1111/jpn.12268
Friedauer K, et al. Detection of 11 Beta-hydroxysteroid Dehydrogenase Type 1, the Glucocorticoid and Mineralocorticoid Receptor in Various Adipose Tissue Depots of Dairy Cows Supplemented With Conjugated Linoleic Acids. J Anim Physiol Anim Nutr (Berl). 2015;99(5):950-61. PubMed PMID: 25354306.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of 11 beta-hydroxysteroid dehydrogenase type 1, the glucocorticoid and mineralocorticoid receptor in various adipose tissue depots of dairy cows supplemented with conjugated linoleic acids. AU - Friedauer,K, AU - Dänicke,S, AU - Schulz,K, AU - Sauerwein,H, AU - Häussler,S, Y1 - 2014/10/29/ PY - 2014/01/22/received PY - 2014/09/19/accepted PY - 2014/10/30/entrez PY - 2014/10/30/pubmed PY - 2016/7/21/medline KW - 11 beta-hydroxysteroid dehydrogenase type 1 KW - conjugated linoleic acids KW - dairy cows KW - glucocorticoid receptor KW - mineralocortioid receptor SP - 950 EP - 61 JF - Journal of animal physiology and animal nutrition JO - J Anim Physiol Anim Nutr (Berl) VL - 99 IS - 5 N2 - Early lactating cows mobilize adipose tissue (AT) to provide energy for milk yield and maintenance and are susceptible to metabolic disorders and impaired immune response. Conjugated linoleic acids (CLA), mainly the trans-10, cis-12 isomer, reduce milk fat synthesis and may attenuate negative energy balance. Circulating glucocorticoids (GC) are increased during parturition in dairy cows and mediate differentiating and anti-inflammatory effects via glucocorticoid (GR) and mineralocorticoid receptors (MR) in the presence of the enzyme 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1). Activated GC are the main ligands for both receptors in AT; therefore, we hypothesized that tissue-specific GC metabolism is effected by varying amounts of GR, MR and 11βHSD1 and/or their localization within AT depots. Furthermore, the lipolytic and antilipogenic effects of CLA might influence the GC/GR/MR system in AT. Therefore, we aimed to localize GR and MR as well as the expression pattern and activity of 11βHSD1 in different AT depots during early lactation in dairy cows and to identify potential effects of CLA. Primiparous German Holstein cows were divided into a control (CON) and a CLA group. From day 1 post-partum (p.p.) until sample collection, the CLA group was fed with 100 g/d CLA (contains 10 g each of the cis-9, trans-11 and the trans-10, cis-12-CLA isomers). CON cows (n = 5 each) were slaughtered on day 1, 42 and 105 p.p., while CLA cows (n = 5 each) were slaughtered on day 42 and 105 p.p. Subcutaneous fat from tailhead, withers and sternum, and visceral fat from omental, mesenteric and retroperitoneal depots were sampled. The localization of GR and 11βHSD1 in mature adipocytes - being already differentiated - indicates that GC promote other effects via GR than differentiation. Moreover, MR were observed in the stromal vascular cell fraction and positively related to the pre-adipocyte marker Pref-1. However, only marginal CLA effects were observed in this study. SN - 1439-0396 UR - https://www.unboundmedicine.com/medline/citation/25354306/Detection_of_11_beta_hydroxysteroid_dehydrogenase_type_1_the_glucocorticoid_and_mineralocorticoid_receptor_in_various_adipose_tissue_depots_of_dairy_cows_supplemented_with_conjugated_linoleic_acids_ L2 - https://doi.org/10.1111/jpn.12268 DB - PRIME DP - Unbound Medicine ER -