Tags

Type your tag names separated by a space and hit enter

From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders.
PLoS One 2014; 9(10):e111132Plos

Abstract

BACKGROUND

Impaired intestinal barrier function, low-grade inflammation and altered neuronal control are reported in functional gastrointestinal disorders. However, the sequence of and causal relation between these events is unclear, necessitating a spontaneous animal model. The aim of this study was to describe the natural history of intestinal permeability, mucosal and neuromuscular inflammation and nitrergic motor neuron function during the lifetime of the BioBreeding (BB) rat.

METHODS

Normoglycemic BB-diabetes prone (DP) and control rats were sacrificed at different ages and jejunum was harvested to characterize intestinal permeability, inflammation and neuromuscular function.

RESULTS

Both structural and functional evidence of increased intestinal permeability was found in young BB-DP rats from the age of 50 days. In older animals, starting in the mucosa from 70 days and in half of the animals also in the muscularis propria from 110 days, an inflammatory reaction, characterized by an influx of polymorphonuclear cells and higher myeloperoxidase activity, was observed. Finally, in animals older than 110 days, coinciding with a myenteric ganglionitis, a loss of nitrergic neurons and motor function was demonstrated.

CONCLUSION

In the BB-rat, mucosal inflammatory cell infiltration is preceded by intestinal barrier dysfunction and followed by myenteric ganglionitis and loss of nitrergic function. This sequence supports a primary role for impaired barrier function and provides an insightful model for the pathogenesis of functional gastrointestinal disorders.

Authors+Show Affiliations

Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.Department of Pathology, University Hospital, University of Leuven, Leuven, Belgium.Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium.Translational Research Center for Gastrointestinal Disorders (TARGID), University of Leuven, Leuven, Belgium; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos II, Barcelona, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25354336

Citation

Vanuytsel, Tim, et al. "From Intestinal Permeability to Dysmotility: the Biobreeding Rat as a Model for Functional Gastrointestinal Disorders." PloS One, vol. 9, no. 10, 2014, pp. e111132.
Vanuytsel T, Vanormelingen C, Vanheel H, et al. From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders. PLoS ONE. 2014;9(10):e111132.
Vanuytsel, T., Vanormelingen, C., Vanheel, H., Masaoka, T., Salim Rasoel, S., Tóth, J., ... Farré, R. (2014). From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders. PloS One, 9(10), pp. e111132. doi:10.1371/journal.pone.0111132.
Vanuytsel T, et al. From Intestinal Permeability to Dysmotility: the Biobreeding Rat as a Model for Functional Gastrointestinal Disorders. PLoS ONE. 2014;9(10):e111132. PubMed PMID: 25354336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - From intestinal permeability to dysmotility: the biobreeding rat as a model for functional gastrointestinal disorders. AU - Vanuytsel,Tim, AU - Vanormelingen,Christophe, AU - Vanheel,Hanne, AU - Masaoka,Tatsuhiro, AU - Salim Rasoel,Shadea, AU - Tóth,Joran, AU - Houben,Els, AU - Verbeke,Kristin, AU - De Hertogh,Gert, AU - Vanden Berghe,Pieter, AU - Tack,Jan, AU - Farré,Ricard, Y1 - 2014/10/29/ PY - 2014/07/21/received PY - 2014/09/19/accepted PY - 2014/10/30/entrez PY - 2014/10/30/pubmed PY - 2015/6/27/medline SP - e111132 EP - e111132 JF - PloS one JO - PLoS ONE VL - 9 IS - 10 N2 - BACKGROUND: Impaired intestinal barrier function, low-grade inflammation and altered neuronal control are reported in functional gastrointestinal disorders. However, the sequence of and causal relation between these events is unclear, necessitating a spontaneous animal model. The aim of this study was to describe the natural history of intestinal permeability, mucosal and neuromuscular inflammation and nitrergic motor neuron function during the lifetime of the BioBreeding (BB) rat. METHODS: Normoglycemic BB-diabetes prone (DP) and control rats were sacrificed at different ages and jejunum was harvested to characterize intestinal permeability, inflammation and neuromuscular function. RESULTS: Both structural and functional evidence of increased intestinal permeability was found in young BB-DP rats from the age of 50 days. In older animals, starting in the mucosa from 70 days and in half of the animals also in the muscularis propria from 110 days, an inflammatory reaction, characterized by an influx of polymorphonuclear cells and higher myeloperoxidase activity, was observed. Finally, in animals older than 110 days, coinciding with a myenteric ganglionitis, a loss of nitrergic neurons and motor function was demonstrated. CONCLUSION: In the BB-rat, mucosal inflammatory cell infiltration is preceded by intestinal barrier dysfunction and followed by myenteric ganglionitis and loss of nitrergic function. This sequence supports a primary role for impaired barrier function and provides an insightful model for the pathogenesis of functional gastrointestinal disorders. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25354336/From_intestinal_permeability_to_dysmotility:_the_biobreeding_rat_as_a_model_for_functional_gastrointestinal_disorders_ L2 - http://dx.plos.org/10.1371/journal.pone.0111132 DB - PRIME DP - Unbound Medicine ER -