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Electrogenerated chemiluminescence resonance energy transfer between luminol and CdSe@ZnS quantum dots and its sensing application in the determination of thrombin.
Anal Chem. 2014 Nov 18; 86(22):11373-9.AC

Abstract

In this work, electrogenerated chemiluminescence resonance energy transfer (ECL-RET) between luminol as a donor and CdSe@ZnS quantum dots (QDs) as an acceptor was reported in neutral conditions. It was observed that a glassy carbon electrode modified with CdSe@ZnS quantum dots (CdSe@ZnS/GCE) can catalyze the luminol oxidation to promote the anodic luminol ECL without coreactants. The intensity of anodic luminol ECL (0.60 V) at the CdSe@ZnS/GCE was enhanced more than 1 order of magnitude compared with that at the bare GCE. Another stronger anodic ECL peak observed at more positive potential (1.10 V) could be assigned to the ECL-RET between the excited state of luminol and the QDs. A label-free ECL aptasensor for the detection of thrombin was fabricated based on the synergic effect of the electrocatalysis and the ECL-RET. The approach showed high sensitivity, good selectivity, and wide linearity for the detection of thrombin in the range of 10 fM-100 pM with a detection limit of 1.4 fM (S/N = 3). The results suggested that the as-proposed luminol-QDs ECL biosensor will be promising in the detection of protein.

Authors+Show Affiliations

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25361206

Citation

Dong, Yong-Ping, et al. "Electrogenerated Chemiluminescence Resonance Energy Transfer Between Luminol and CdSe@ZnS Quantum Dots and Its Sensing Application in the Determination of Thrombin." Analytical Chemistry, vol. 86, no. 22, 2014, pp. 11373-9.
Dong YP, Gao TT, Zhou Y, et al. Electrogenerated chemiluminescence resonance energy transfer between luminol and CdSe@ZnS quantum dots and its sensing application in the determination of thrombin. Anal Chem. 2014;86(22):11373-9.
Dong, Y. P., Gao, T. T., Zhou, Y., & Zhu, J. J. (2014). Electrogenerated chemiluminescence resonance energy transfer between luminol and CdSe@ZnS quantum dots and its sensing application in the determination of thrombin. Analytical Chemistry, 86(22), 11373-9. https://doi.org/10.1021/ac5033319
Dong YP, et al. Electrogenerated Chemiluminescence Resonance Energy Transfer Between Luminol and CdSe@ZnS Quantum Dots and Its Sensing Application in the Determination of Thrombin. Anal Chem. 2014 Nov 18;86(22):11373-9. PubMed PMID: 25361206.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrogenerated chemiluminescence resonance energy transfer between luminol and CdSe@ZnS quantum dots and its sensing application in the determination of thrombin. AU - Dong,Yong-Ping, AU - Gao,Ting-Ting, AU - Zhou,Ying, AU - Zhu,Jun-Jie, Y1 - 2014/11/07/ PY - 2014/11/1/entrez PY - 2014/11/2/pubmed PY - 2015/9/9/medline SP - 11373 EP - 9 JF - Analytical chemistry JO - Anal Chem VL - 86 IS - 22 N2 - In this work, electrogenerated chemiluminescence resonance energy transfer (ECL-RET) between luminol as a donor and CdSe@ZnS quantum dots (QDs) as an acceptor was reported in neutral conditions. It was observed that a glassy carbon electrode modified with CdSe@ZnS quantum dots (CdSe@ZnS/GCE) can catalyze the luminol oxidation to promote the anodic luminol ECL without coreactants. The intensity of anodic luminol ECL (0.60 V) at the CdSe@ZnS/GCE was enhanced more than 1 order of magnitude compared with that at the bare GCE. Another stronger anodic ECL peak observed at more positive potential (1.10 V) could be assigned to the ECL-RET between the excited state of luminol and the QDs. A label-free ECL aptasensor for the detection of thrombin was fabricated based on the synergic effect of the electrocatalysis and the ECL-RET. The approach showed high sensitivity, good selectivity, and wide linearity for the detection of thrombin in the range of 10 fM-100 pM with a detection limit of 1.4 fM (S/N = 3). The results suggested that the as-proposed luminol-QDs ECL biosensor will be promising in the detection of protein. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/25361206/Electrogenerated_chemiluminescence_resonance_energy_transfer_between_luminol_and_CdSe@ZnS_quantum_dots_and_its_sensing_application_in_the_determination_of_thrombin_ L2 - https://doi.org/10.1021/ac5033319 DB - PRIME DP - Unbound Medicine ER -