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The expression of GPR109A, NF-kB and IL-1β in peripheral blood leukocytes from patients with type 2 diabetes.
Ann Clin Lab Sci. 2014 Fall; 44(4):443-8.AC

Abstract

This study was designed to explore the association between the G protein-coupled receptor 109A (GPR109A) expression in peripheral blood leukocytes (PBLs) and type 2 diabetes (T2DM) and to discuss the regulation of inflammatory factors by GPR109A signaling. GPR109A signaling has been confirmed to be associated with homeostasis of glucose/lipid metabolism, but the role of signaling in T2DM is still poorly understood. Peripheral blood samples and biochemical data were collected from healthy individuals (normal controls) and T2DM patients. Immunocytochemical staining was used to detect the expression of GPR109A in PBLs. Reverse transcription polymerase chain reaction (RT-PCR) was used to measure mRNA levels of GPR109A, NF-κB, and IL-1β in PBLs. Immunocytochemical staining showed that the GPR109A protein is localized in the nucleus and cytoplasm of granulocytes, monocytes, and lymphocytes. RT-PCR showed that mRNA levels of GPR109A, NF-κB, and IL-1β were higher in the T2DM group than in the control group (P<0.05). Correlation analysis showed a positive correlation both between GPR109A/NF-κB (r=0.376, P<0.05), and GPR109A/IL-1β (r=501, P<0.05) and between GPR109A and fasting plasma glucose (FPG) (r=0.179, P<0.05) and NF-κB /FPG (r=0.358, p<0.05). Our results suggest that GPR109A signaling is associated with T2DM, playing a role in regulation of the inflammatory cytokines.

Authors+Show Affiliations

Department of Endocrinology, the First Affiliated Hospital of Shantou University Medical College, Shantou Laboratory of Cell Senescence, Shantou University Medical College, Shantou, China.Laboratory of Cell Senescence, Shantou University Medical College, Shantou, China.Laboratory of Cell Senescence, Shantou University Medical College, Shantou, China.Department of Endocrinology, the First Affiliated Hospital of Shantou University Medical College, Shantou.Department of Endocrinology, the First Affiliated Hospital of Shantou University Medical College, Shantou.Department of Endocrinology, the First Affiliated Hospital of Shantou University Medical College, Shantou xuwcan@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25361930

Citation

Liu, Fengxiu, et al. "The Expression of GPR109A, NF-kB and IL-1β in Peripheral Blood Leukocytes From Patients With Type 2 Diabetes." Annals of Clinical and Laboratory Science, vol. 44, no. 4, 2014, pp. 443-8.
Liu F, Fu Y, Wei C, et al. The expression of GPR109A, NF-kB and IL-1β in peripheral blood leukocytes from patients with type 2 diabetes. Ann Clin Lab Sci. 2014;44(4):443-8.
Liu, F., Fu, Y., Wei, C., Chen, Y., Ma, S., & Xu, W. (2014). The expression of GPR109A, NF-kB and IL-1β in peripheral blood leukocytes from patients with type 2 diabetes. Annals of Clinical and Laboratory Science, 44(4), 443-8.
Liu F, et al. The Expression of GPR109A, NF-kB and IL-1β in Peripheral Blood Leukocytes From Patients With Type 2 Diabetes. Ann Clin Lab Sci. 2014;44(4):443-8. PubMed PMID: 25361930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The expression of GPR109A, NF-kB and IL-1β in peripheral blood leukocytes from patients with type 2 diabetes. AU - Liu,Fengxiu, AU - Fu,Yucai, AU - Wei,Chiju, AU - Chen,Yongru, AU - Ma,Shuhua, AU - Xu,Wencan, PY - 2014/11/2/entrez PY - 2014/11/2/pubmed PY - 2015/6/24/medline KW - GPR109A KW - IL-1β KW - NF-κB KW - Pancreatic islet β-cell KW - Type 2 diabetes KW - peripheral blood leukocytes SP - 443 EP - 8 JF - Annals of clinical and laboratory science JO - Ann Clin Lab Sci VL - 44 IS - 4 N2 - This study was designed to explore the association between the G protein-coupled receptor 109A (GPR109A) expression in peripheral blood leukocytes (PBLs) and type 2 diabetes (T2DM) and to discuss the regulation of inflammatory factors by GPR109A signaling. GPR109A signaling has been confirmed to be associated with homeostasis of glucose/lipid metabolism, but the role of signaling in T2DM is still poorly understood. Peripheral blood samples and biochemical data were collected from healthy individuals (normal controls) and T2DM patients. Immunocytochemical staining was used to detect the expression of GPR109A in PBLs. Reverse transcription polymerase chain reaction (RT-PCR) was used to measure mRNA levels of GPR109A, NF-κB, and IL-1β in PBLs. Immunocytochemical staining showed that the GPR109A protein is localized in the nucleus and cytoplasm of granulocytes, monocytes, and lymphocytes. RT-PCR showed that mRNA levels of GPR109A, NF-κB, and IL-1β were higher in the T2DM group than in the control group (P<0.05). Correlation analysis showed a positive correlation both between GPR109A/NF-κB (r=0.376, P<0.05), and GPR109A/IL-1β (r=501, P<0.05) and between GPR109A and fasting plasma glucose (FPG) (r=0.179, P<0.05) and NF-κB /FPG (r=0.358, p<0.05). Our results suggest that GPR109A signaling is associated with T2DM, playing a role in regulation of the inflammatory cytokines. SN - 1550-8080 UR - https://www.unboundmedicine.com/medline/citation/25361930/The_expression_of_GPR109A_NF_kB_and_IL_1β_in_peripheral_blood_leukocytes_from_patients_with_type_2_diabetes_ L2 - http://www.annclinlabsci.org/cgi/pmidlookup?view=long&amp;pmid=25361930 DB - PRIME DP - Unbound Medicine ER -