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Reevaluation of confirmatory tests for human T-cell leukemia virus Type 1 using a luciferase immunoprecipitation system in blood donors.
Transfusion. 2015 Apr; 55(4):880-9.T

Abstract

BACKGROUND

Recently, Japanese Red Cross blood centers have changed the confirmatory test method from an indirect immunofluorescence (IF) technique to Western blotting (WB) for antibodies against human T-cell leukemia virus Type 1 (HTLV-1). In this study, these HTLV-1 tests were assessed using another sensitive method, that is, a luciferase immunoprecipitation system (LIPS), to identify a better confirmatory test for HTLV-1 infection.

STUDY DESIGN AND METHODS

Plasma samples from 54 qualified donors and 114 HTLV-1 screening-positive donors were tested by LIPS for antibodies against HTLV-1 Gag, Tax, Env, and HBZ recombinant proteins. The donors were categorized into six groups, namely, (Group I) qualified donors, screening positive; (Group II) IF positive; (Group III) IF negative; (Group IV) WB positive; (Group V) WB negative; and (Group VI) screening positive in the previous blood donation, but WB-indeterminate during this study period.

RESULTS

In Groups II and IV, all plasma samples tested positive by LIPS for antibodies against Gag and Env proteins. In Group V, all samples tested negative by LIPS, whereas some Group III samples reacted with single or double antigens in LIPS. In Group VI, the LIPS test identified a donor with suspected HTLV-1 infection. The first case of a blood donor with plasma that reacted with HBZ was identified by LIPS.

CONCLUSION

Reevaluation of the current HTLV-1 screening method using the LIPS test showed that both confirmatory tests had similar sensitivity and specificity only when WB indeterminate results were eliminated. LIPS is a promising method for detecting and characterizing HTLV-1 antibodies.

Authors+Show Affiliations

Japanese Red Cross, Kinki Block Blood Center, Osaka, Japan.Laboratory of Virus Control, Institute for Virus Research, Kyoto University, Kyoto, Japan.Laboratory of Virus Control, Institute for Virus Research, Kyoto University, Kyoto, Japan.Japanese Red Cross, Kinki Block Blood Center, Osaka, Japan.Japanese Red Cross, Kinki Block Blood Center, Osaka, Japan.Japanese Red Cross, Kinki Block Blood Center, Osaka, Japan.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25363675

Citation

Furuta, Rika A., et al. "Reevaluation of Confirmatory Tests for Human T-cell Leukemia Virus Type 1 Using a Luciferase Immunoprecipitation System in Blood Donors." Transfusion, vol. 55, no. 4, 2015, pp. 880-9.
Furuta RA, Ma G, Matsuoka M, et al. Reevaluation of confirmatory tests for human T-cell leukemia virus Type 1 using a luciferase immunoprecipitation system in blood donors. Transfusion. 2015;55(4):880-9.
Furuta, R. A., Ma, G., Matsuoka, M., Otani, S., Matsukura, H., & Hirayama, F. (2015). Reevaluation of confirmatory tests for human T-cell leukemia virus Type 1 using a luciferase immunoprecipitation system in blood donors. Transfusion, 55(4), 880-9. https://doi.org/10.1111/trf.12911
Furuta RA, et al. Reevaluation of Confirmatory Tests for Human T-cell Leukemia Virus Type 1 Using a Luciferase Immunoprecipitation System in Blood Donors. Transfusion. 2015;55(4):880-9. PubMed PMID: 25363675.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reevaluation of confirmatory tests for human T-cell leukemia virus Type 1 using a luciferase immunoprecipitation system in blood donors. AU - Furuta,Rika A, AU - Ma,Guangyong, AU - Matsuoka,Masao, AU - Otani,Satoshi, AU - Matsukura,Harumichi, AU - Hirayama,Fumiya, Y1 - 2014/11/02/ PY - 2014/05/14/received PY - 2014/08/21/revised PY - 2014/09/06/accepted PY - 2014/11/4/entrez PY - 2014/11/5/pubmed PY - 2016/6/23/medline SP - 880 EP - 9 JF - Transfusion JO - Transfusion VL - 55 IS - 4 N2 - BACKGROUND: Recently, Japanese Red Cross blood centers have changed the confirmatory test method from an indirect immunofluorescence (IF) technique to Western blotting (WB) for antibodies against human T-cell leukemia virus Type 1 (HTLV-1). In this study, these HTLV-1 tests were assessed using another sensitive method, that is, a luciferase immunoprecipitation system (LIPS), to identify a better confirmatory test for HTLV-1 infection. STUDY DESIGN AND METHODS: Plasma samples from 54 qualified donors and 114 HTLV-1 screening-positive donors were tested by LIPS for antibodies against HTLV-1 Gag, Tax, Env, and HBZ recombinant proteins. The donors were categorized into six groups, namely, (Group I) qualified donors, screening positive; (Group II) IF positive; (Group III) IF negative; (Group IV) WB positive; (Group V) WB negative; and (Group VI) screening positive in the previous blood donation, but WB-indeterminate during this study period. RESULTS: In Groups II and IV, all plasma samples tested positive by LIPS for antibodies against Gag and Env proteins. In Group V, all samples tested negative by LIPS, whereas some Group III samples reacted with single or double antigens in LIPS. In Group VI, the LIPS test identified a donor with suspected HTLV-1 infection. The first case of a blood donor with plasma that reacted with HBZ was identified by LIPS. CONCLUSION: Reevaluation of the current HTLV-1 screening method using the LIPS test showed that both confirmatory tests had similar sensitivity and specificity only when WB indeterminate results were eliminated. LIPS is a promising method for detecting and characterizing HTLV-1 antibodies. SN - 1537-2995 UR - https://www.unboundmedicine.com/medline/citation/25363675/Reevaluation_of_confirmatory_tests_for_human_T_cell_leukemia_virus_Type_1_using_a_luciferase_immunoprecipitation_system_in_blood_donors_ L2 - https://doi.org/10.1111/trf.12911 DB - PRIME DP - Unbound Medicine ER -