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Developmental validation of the AGCU 21+1 STR kit: a novel multiplex assay for forensic application.
Electrophoresis. 2015 Jan; 36(2):271-6.E

Abstract

In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex-determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application.

Authors+Show Affiliations

Research Center of Stomatology, Stomatology Hospital, Xi'an Jiaotong University, Xi'an, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

25363755

Citation

Zhu, Bo-Feng, et al. "Developmental Validation of the AGCU 21+1 STR Kit: a Novel Multiplex Assay for Forensic Application." Electrophoresis, vol. 36, no. 2, 2015, pp. 271-6.
Zhu BF, Zhang YD, Shen CM, et al. Developmental validation of the AGCU 21+1 STR kit: a novel multiplex assay for forensic application. Electrophoresis. 2015;36(2):271-6.
Zhu, B. F., Zhang, Y. D., Shen, C. M., Du, W. A., Liu, W. J., Meng, H. T., Wang, H. D., Yang, G., Jin, R., Yang, C. H., Yan, J. W., & Bie, X. H. (2015). Developmental validation of the AGCU 21+1 STR kit: a novel multiplex assay for forensic application. Electrophoresis, 36(2), 271-6. https://doi.org/10.1002/elps.201400333
Zhu BF, et al. Developmental Validation of the AGCU 21+1 STR Kit: a Novel Multiplex Assay for Forensic Application. Electrophoresis. 2015;36(2):271-6. PubMed PMID: 25363755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental validation of the AGCU 21+1 STR kit: a novel multiplex assay for forensic application. AU - Zhu,Bo-Feng, AU - Zhang,Yu-Dang, AU - Shen,Chun-Mei, AU - Du,Wei-An, AU - Liu,Wen-Juan, AU - Meng,Hao-Tian, AU - Wang,Hong-Dan, AU - Yang,Guang, AU - Jin,Rui, AU - Yang,Chun-Hua, AU - Yan,Jiang-Wei, AU - Bie,Xiao-Hua, Y1 - 2014/12/17/ PY - 2014/07/12/received PY - 2014/09/21/revised PY - 2014/10/15/accepted PY - 2014/11/4/entrez PY - 2014/11/5/pubmed PY - 2015/8/5/medline KW - AGCU 21+1 STR kit KW - DNA genotyping KW - Forensic validation KW - Short tandem repeat SP - 271 EP - 6 JF - Electrophoresis JO - Electrophoresis VL - 36 IS - 2 N2 - In this study, we describe the developmental validation assay performed on a novel designed STR multiplex system, AGCU 21+1 STR kit. This kit contains a sex-determining locus amelogenin and 21 noncombined DNA index system STR loci, that are, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, and D5S2500. The 21+1 kit was validated by a series of tests including optimized PCR conditions, sensitivity, precision and accuracy, stutter ratio, DNA mixture, inhibitors, and species specificity according to the revised validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our results in this study show that the kit is a useful tool for forensic application. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/25363755/Developmental_validation_of_the_AGCU_21+1_STR_kit:_a_novel_multiplex_assay_for_forensic_application_ DB - PRIME DP - Unbound Medicine ER -