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Impact of pregabalin treatment on synaptic plasticity and glial reactivity during the course of experimental autoimmune encephalomyelitis.
Brain Behav. 2014; 4(6):925-35.BB

Abstract

BACKGROUND

Multiple sclerosis (MS) is an autoimmune and neurodegenerative disease that affects young adults. It is characterized by generating a chronic demyelinating autoimmune inflammation in the central nervous system. An experimental model for studying MS is the experimental autoimmune encephalomyelitis (EAE), induced by immunization with antigenic proteins from myelin.

AIMS

The present study investigated the evolution of EAE in pregabalin treated animals up to the remission phase.

METHODS AND RESULTS

The results demonstrated a delay in the onset of the disease with statistical differences at the 10th and the 16th day after immunization. Additionally, the walking track test (CatWalk) was used to evaluate different parameters related to motor function. Although no difference between groups was obtained for the foot print pressure, the regularity index was improved post treatment, indicating a better motor coordination. The immunohistochemical analysis of putative synapse preservation and glial reactivity revealed that pregabalin treatment improved the overall morphology of the spinal cord. A preservation of circuits was depicted and the glial reaction was downregulated during the course of the disease. qRT-PCR data did not show immunomodulatory effects of pregabalin, indicating that the positive effects were restricted to the CNS environment.

CONCLUSIONS

Overall, the present data indicate that pregabalin is efficient for reducing the seriousness of EAE, delaying its course as well as reducing synaptic loss and astroglial reaction.

Authors+Show Affiliations

Laboratory of Nerve Regeneration, Department of Structural and Functional Biology, Institute of Biology, University of Campinas - UNICAMP Campinas, SP, Brazil.Neuroimmunology Unit, Department of Genetics, Evolution and Bioagents, University of Campinas - UNICAMP Campinas, SP, Brazil ; Neuroimmunomodulation Group, Department of Genetics, Evolution and Bioagents, University of Campinas - UNICAMP Campinas, SP, Brazil.Neuroimmunology Unit, Department of Genetics, Evolution and Bioagents, University of Campinas - UNICAMP Campinas, SP, Brazil ; Neuroimmunomodulation Group, Department of Genetics, Evolution and Bioagents, University of Campinas - UNICAMP Campinas, SP, Brazil.Neuroimmunology Unit, Department of Genetics, Evolution and Bioagents, University of Campinas - UNICAMP Campinas, SP, Brazil ; Neuroimmunomodulation Group, Department of Genetics, Evolution and Bioagents, University of Campinas - UNICAMP Campinas, SP, Brazil.Neuroimmunology Unit, Department of Genetics, Evolution and Bioagents, University of Campinas - UNICAMP Campinas, SP, Brazil.Laboratory of Nerve Regeneration, Department of Structural and Functional Biology, Institute of Biology, University of Campinas - UNICAMP Campinas, SP, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25365796

Citation

Silva, Gleidy A A., et al. "Impact of Pregabalin Treatment On Synaptic Plasticity and Glial Reactivity During the Course of Experimental Autoimmune Encephalomyelitis." Brain and Behavior, vol. 4, no. 6, 2014, pp. 925-35.
Silva GA, Pradella F, Moraes A, et al. Impact of pregabalin treatment on synaptic plasticity and glial reactivity during the course of experimental autoimmune encephalomyelitis. Brain Behav. 2014;4(6):925-35.
Silva, G. A., Pradella, F., Moraes, A., Farias, A., dos Santos, L. M., & de Oliveira, A. L. (2014). Impact of pregabalin treatment on synaptic plasticity and glial reactivity during the course of experimental autoimmune encephalomyelitis. Brain and Behavior, 4(6), 925-35. https://doi.org/10.1002/brb3.276
Silva GA, et al. Impact of Pregabalin Treatment On Synaptic Plasticity and Glial Reactivity During the Course of Experimental Autoimmune Encephalomyelitis. Brain Behav. 2014;4(6):925-35. PubMed PMID: 25365796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of pregabalin treatment on synaptic plasticity and glial reactivity during the course of experimental autoimmune encephalomyelitis. AU - Silva,Gleidy A A, AU - Pradella,Fernando, AU - Moraes,Adriel, AU - Farias,Alessandro, AU - dos Santos,Leonilda M B, AU - de Oliveira,Alexandre L R, Y1 - 2014/09/02/ PY - 2014/05/01/received PY - 2014/07/22/revised PY - 2014/07/29/accepted PY - 2014/11/4/entrez PY - 2014/11/5/pubmed PY - 2015/7/25/medline KW - Experimental autoimmune encephalomyelitis KW - pregabalin KW - spinal motoneurons KW - synapse SP - 925 EP - 35 JF - Brain and behavior JO - Brain Behav VL - 4 IS - 6 N2 - BACKGROUND: Multiple sclerosis (MS) is an autoimmune and neurodegenerative disease that affects young adults. It is characterized by generating a chronic demyelinating autoimmune inflammation in the central nervous system. An experimental model for studying MS is the experimental autoimmune encephalomyelitis (EAE), induced by immunization with antigenic proteins from myelin. AIMS: The present study investigated the evolution of EAE in pregabalin treated animals up to the remission phase. METHODS AND RESULTS: The results demonstrated a delay in the onset of the disease with statistical differences at the 10th and the 16th day after immunization. Additionally, the walking track test (CatWalk) was used to evaluate different parameters related to motor function. Although no difference between groups was obtained for the foot print pressure, the regularity index was improved post treatment, indicating a better motor coordination. The immunohistochemical analysis of putative synapse preservation and glial reactivity revealed that pregabalin treatment improved the overall morphology of the spinal cord. A preservation of circuits was depicted and the glial reaction was downregulated during the course of the disease. qRT-PCR data did not show immunomodulatory effects of pregabalin, indicating that the positive effects were restricted to the CNS environment. CONCLUSIONS: Overall, the present data indicate that pregabalin is efficient for reducing the seriousness of EAE, delaying its course as well as reducing synaptic loss and astroglial reaction. SN - 2162-3279 UR - https://www.unboundmedicine.com/medline/citation/25365796/Impact_of_pregabalin_treatment_on_synaptic_plasticity_and_glial_reactivity_during_the_course_of_experimental_autoimmune_encephalomyelitis_ L2 - https://doi.org/10.1002/brb3.276 DB - PRIME DP - Unbound Medicine ER -