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A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage.
J Bacteriol. 1989 Feb; 171(2):744-53.JB

Abstract

A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the molecular level. Nucleotide sequence determination revealed the presence of two open reading frames. Both open reading frames were found by deletion and complementation analysis to be necessary for expression of the hemolytic phenotype by Bacillus subtilis and Escherichia coli hosts. The 5' open reading frame was found to be nearly identical to a recently reported phospholipase C gene derived from a mutant B. cereus strain which overexpresses the respective protein, and it conferred a lecithinase-positive phenotype to the B. subtilis host. The 3' open reading frame encoded a sphingomyelinase. The two tandemly encoded activities, phospholipase C and sphingomyelinase, constitute a biologically functional cytolytic determinant of B. cereus termed cereolysin AB.

Authors+Show Affiliations

Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

2536680

Citation

Gilmore, M S., et al. "A Bacillus Cereus Cytolytic Determinant, Cereolysin AB, Which Comprises the Phospholipase C and Sphingomyelinase Genes: Nucleotide Sequence and Genetic Linkage." Journal of Bacteriology, vol. 171, no. 2, 1989, pp. 744-53.
Gilmore MS, Cruz-Rodz AL, Leimeister-Wächter M, et al. A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage. J Bacteriol. 1989;171(2):744-53.
Gilmore, M. S., Cruz-Rodz, A. L., Leimeister-Wächter, M., Kreft, J., & Goebel, W. (1989). A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage. Journal of Bacteriology, 171(2), 744-53.
Gilmore MS, et al. A Bacillus Cereus Cytolytic Determinant, Cereolysin AB, Which Comprises the Phospholipase C and Sphingomyelinase Genes: Nucleotide Sequence and Genetic Linkage. J Bacteriol. 1989;171(2):744-53. PubMed PMID: 2536680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage. AU - Gilmore,M S, AU - Cruz-Rodz,A L, AU - Leimeister-Wächter,M, AU - Kreft,J, AU - Goebel,W, PY - 1989/2/1/pubmed PY - 1989/2/1/medline PY - 1989/2/1/entrez SP - 744 EP - 53 JF - Journal of bacteriology JO - J. Bacteriol. VL - 171 IS - 2 N2 - A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the molecular level. Nucleotide sequence determination revealed the presence of two open reading frames. Both open reading frames were found by deletion and complementation analysis to be necessary for expression of the hemolytic phenotype by Bacillus subtilis and Escherichia coli hosts. The 5' open reading frame was found to be nearly identical to a recently reported phospholipase C gene derived from a mutant B. cereus strain which overexpresses the respective protein, and it conferred a lecithinase-positive phenotype to the B. subtilis host. The 3' open reading frame encoded a sphingomyelinase. The two tandemly encoded activities, phospholipase C and sphingomyelinase, constitute a biologically functional cytolytic determinant of B. cereus termed cereolysin AB. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/2536680/A_Bacillus_cereus_cytolytic_determinant_cereolysin_AB_which_comprises_the_phospholipase_C_and_sphingomyelinase_genes:_nucleotide_sequence_and_genetic_linkage_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=2536680 DB - PRIME DP - Unbound Medicine ER -