Tags

Type your tag names separated by a space and hit enter

A novel bioassay to evaluate the potential of Beauveria bassiana strain NI8 and the insect growth regulator novaluron against Lygus lineolaris on a non-autoclaved solid artificial diet.
J Insect Sci. 2014; 14:115.JI

Abstract

A non-autoclaved solid diet was used to evaluate the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Clavicipitaceae) strain NI8 and the insect growth regulator novaluron (Diamond® 0.83EC insecticide) for control of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae). The diet was composed of toasted wheat germ, ground lima bean meal, soy flour, yolk of chicken eggs, inhibitor, and agar. It was prepared in one step by blending the ingredients in boiling water. The diet was used to bioassay L. lineolaris from the second instar to the adult stage. Fourth and fifth instars and adults of L. lineolaris were more susceptible than second and third instars to infection by B. bassiana, whereas second, third, and fourth instars had higher mortality than fifth instars 10 days after exposure to novaluron. No effects on longevity were observed in adults treated with novaluron when compared with the control, but longevity was significantly different from that of adults exposed to B. bassiana. Adults of L. lineolaris were maintained for over a month without changing the diet. The non-autoclaved diet is semi-liquid before it cools, which facilitates the mechanics of diet packaging similar to food packaging or lepidopteran diet preparation. This solid artificial diet for Lygus bugs provides improved research capacity for studying the ecology and susceptibility of Lygus spp. to a number of different control agents, including beneficial organisms, insect pathogens, and insecticidal toxins being developed for transgenic technologies.

Links

PMC Free PDF

Authors+Show Affiliations

Portilla M
U.S. Department of Agriculture, Agricultural Research Service, Stoneville, MS 38732 maribel.portilla@ars.usda.gov.
Snodgrass G
U.S. Department of Agriculture, Agricultural Research Service, Stoneville, MS 38732.
Luttrell R
U.S. Department of Agriculture, Agricultural Research Service, Stoneville, MS 38732.
Jaronski S
U.S. Department of Agriculture, Agricultural Research Service, Stoneville, MS 38732.

MeSH

AgingAnimalsBeauveriaBiological AssayDietHemipteraJuvenile HormonesNymphPhenylurea Compounds

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

25368059

Citation

Portilla, Maribel, et al. "A Novel Bioassay to Evaluate the Potential of Beauveria Bassiana Strain NI8 and the Insect Growth Regulator Novaluron Against Lygus Lineolaris On a Non-autoclaved Solid Artificial Diet." Journal of Insect Science (Online), vol. 14, 2014, p. 115.
Portilla M, Snodgrass G, Luttrell R, et al. A novel bioassay to evaluate the potential of Beauveria bassiana strain NI8 and the insect growth regulator novaluron against Lygus lineolaris on a non-autoclaved solid artificial diet. J Insect Sci. 2014;14:115.
Portilla, M., Snodgrass, G., Luttrell, R., & Jaronski, S. (2014). A novel bioassay to evaluate the potential of Beauveria bassiana strain NI8 and the insect growth regulator novaluron against Lygus lineolaris on a non-autoclaved solid artificial diet. Journal of Insect Science (Online), 14, 115. https://doi.org/10.1093/jis/14.1.115
Portilla M, et al. A Novel Bioassay to Evaluate the Potential of Beauveria Bassiana Strain NI8 and the Insect Growth Regulator Novaluron Against Lygus Lineolaris On a Non-autoclaved Solid Artificial Diet. J Insect Sci. 2014;14:115. PubMed PMID: 25368059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel bioassay to evaluate the potential of Beauveria bassiana strain NI8 and the insect growth regulator novaluron against Lygus lineolaris on a non-autoclaved solid artificial diet. AU - Portilla,Maribel, AU - Snodgrass,Gordon, AU - Luttrell,Randall, AU - Jaronski,Stefan, PY - 2014/11/5/entrez PY - 2014/11/5/pubmed PY - 2016/4/30/medline KW - bioassay KW - biocontrol SP - 115 EP - 115 JF - Journal of insect science (Online) JO - J Insect Sci VL - 14 N2 - A non-autoclaved solid diet was used to evaluate the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Clavicipitaceae) strain NI8 and the insect growth regulator novaluron (Diamond® 0.83EC insecticide) for control of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae). The diet was composed of toasted wheat germ, ground lima bean meal, soy flour, yolk of chicken eggs, inhibitor, and agar. It was prepared in one step by blending the ingredients in boiling water. The diet was used to bioassay L. lineolaris from the second instar to the adult stage. Fourth and fifth instars and adults of L. lineolaris were more susceptible than second and third instars to infection by B. bassiana, whereas second, third, and fourth instars had higher mortality than fifth instars 10 days after exposure to novaluron. No effects on longevity were observed in adults treated with novaluron when compared with the control, but longevity was significantly different from that of adults exposed to B. bassiana. Adults of L. lineolaris were maintained for over a month without changing the diet. The non-autoclaved diet is semi-liquid before it cools, which facilitates the mechanics of diet packaging similar to food packaging or lepidopteran diet preparation. This solid artificial diet for Lygus bugs provides improved research capacity for studying the ecology and susceptibility of Lygus spp. to a number of different control agents, including beneficial organisms, insect pathogens, and insecticidal toxins being developed for transgenic technologies. SN - 1536-2442 UR - https://www.unboundmedicine.com/medline/citation/25368059/A_novel_bioassay_to_evaluate_the_potential_of_Beauveria_bassiana_strain_NI8_and_the_insect_growth_regulator_novaluron_against_Lygus_lineolaris_on_a_non_autoclaved_solid_artificial_diet_ DB - PRIME DP - Unbound Medicine ER -