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Inhibitory effect of gut bacteria from the Japanese honey bee, Apis cerana japonica, against Melissococcus plutonius, the causal agent of European foulbrood disease.
J Insect Sci. 2014; 14:129.JI

Abstract

European foulbrood is a contagious bacterial disease of honey bee larvae. Studies have shown that the intestinal bacteria of insects, including honey bees, act as probiotic organisms. Microbial flora from the gut of the Japanese honey bee, Apis cerana japonica F. (Hymenoptera: Apidae), were characterized and evaluated for their potential to inhibit the growth of Melissococcus plutonius corrig. (ex White) Bailey and Collins (Lactobacillales: Enterococcaceae), the causative agent of European foulbrood. Analysis of 16S rRNA gene sequences from 17 bacterial strains isolated by using a culture-dependent method revealed that most isolates belonged to Bacillus, Staphylococcus, and Pantoea. The isolates were screened against the pathogenic bacterium M. plutonius by using an in vitro growth inhibition assay, and one isolate (Acja3) belonging to the genus Bacillus exhibited inhibitory activity against M. plutonius. In addition, in vivo feeding assays revealed that isolate Acja3 decreased the mortality of honey bee larvae infected with M plutonius, suggesting that this bacterial strain could potentially be used as a probiotic agent against European foulbrood.

Authors+Show Affiliations

Honey Bee Research Unit, Animal Breeding and Reproduction Research Division, National Institute of Livestock and Grassland Science, NARO, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan tanghis.wu@gmail.com.Honey Bee Research Unit, Animal Breeding and Reproduction Research Division, National Institute of Livestock and Grassland Science, NARO, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan.Honey Bee Research Unit, Animal Breeding and Reproduction Research Division, National Institute of Livestock and Grassland Science, NARO, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan.Honey Bee Research Unit, Animal Breeding and Reproduction Research Division, National Institute of Livestock and Grassland Science, NARO, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan.Bacterial and Parasitic Diseases Research Division, National Institute of Animal Health, NARO, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Gifu 501-1193, Japan.Technical Services for Diagnosis, Diagnostic Section, Center for Animal Disease Control and Prevention, National Institute of Animal Health, NARO, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.Honey Bee Research Unit, Animal Breeding and Reproduction Research Division, National Institute of Livestock and Grassland Science, NARO, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.Honey Bee Research Unit, Animal Breeding and Reproduction Research Division, National Institute of Livestock and Grassland Science, NARO, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25368073

Citation

Wu, Meihua, et al. "Inhibitory Effect of Gut Bacteria From the Japanese Honey Bee, Apis Cerana Japonica, Against Melissococcus Plutonius, the Causal Agent of European Foulbrood Disease." Journal of Insect Science (Online), vol. 14, 2014, p. 129.
Wu M, Sugimura Y, Iwata K, et al. Inhibitory effect of gut bacteria from the Japanese honey bee, Apis cerana japonica, against Melissococcus plutonius, the causal agent of European foulbrood disease. J Insect Sci. 2014;14:129.
Wu, M., Sugimura, Y., Iwata, K., Takaya, N., Takamatsu, D., Kobayashi, M., Taylor, D., Kimura, K., & Yoshiyama, M. (2014). Inhibitory effect of gut bacteria from the Japanese honey bee, Apis cerana japonica, against Melissococcus plutonius, the causal agent of European foulbrood disease. Journal of Insect Science (Online), 14, 129. https://doi.org/10.1093/jis/14.1.129
Wu M, et al. Inhibitory Effect of Gut Bacteria From the Japanese Honey Bee, Apis Cerana Japonica, Against Melissococcus Plutonius, the Causal Agent of European Foulbrood Disease. J Insect Sci. 2014;14:129. PubMed PMID: 25368073.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of gut bacteria from the Japanese honey bee, Apis cerana japonica, against Melissococcus plutonius, the causal agent of European foulbrood disease. AU - Wu,Meihua, AU - Sugimura,Yuya, AU - Iwata,Kyoko, AU - Takaya,Noriko, AU - Takamatsu,Daisuke, AU - Kobayashi,Masaru, AU - Taylor,DeMar, AU - Kimura,Kiyoshi, AU - Yoshiyama,Mikio, PY - 2014/11/5/entrez PY - 2014/11/5/pubmed PY - 2016/2/5/medline KW - honey bee disease KW - microbial flora KW - probiotic SP - 129 EP - 129 JF - Journal of insect science (Online) JO - J Insect Sci VL - 14 N2 - European foulbrood is a contagious bacterial disease of honey bee larvae. Studies have shown that the intestinal bacteria of insects, including honey bees, act as probiotic organisms. Microbial flora from the gut of the Japanese honey bee, Apis cerana japonica F. (Hymenoptera: Apidae), were characterized and evaluated for their potential to inhibit the growth of Melissococcus plutonius corrig. (ex White) Bailey and Collins (Lactobacillales: Enterococcaceae), the causative agent of European foulbrood. Analysis of 16S rRNA gene sequences from 17 bacterial strains isolated by using a culture-dependent method revealed that most isolates belonged to Bacillus, Staphylococcus, and Pantoea. The isolates were screened against the pathogenic bacterium M. plutonius by using an in vitro growth inhibition assay, and one isolate (Acja3) belonging to the genus Bacillus exhibited inhibitory activity against M. plutonius. In addition, in vivo feeding assays revealed that isolate Acja3 decreased the mortality of honey bee larvae infected with M plutonius, suggesting that this bacterial strain could potentially be used as a probiotic agent against European foulbrood. SN - 1536-2442 UR - https://www.unboundmedicine.com/medline/citation/25368073/Inhibitory_effect_of_gut_bacteria_from_the_Japanese_honey_bee_Apis_cerana_japonica_against_Melissococcus_plutonius_the_causal_agent_of_European_foulbrood_disease_ L2 - https://academic.oup.com/jinsectscience/article-lookup/doi/10.1093/jis/14.1.129 DB - PRIME DP - Unbound Medicine ER -