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Determination of polyphenolic profiles by liquid chromatography-electrospray-tandem mass spectrometry for the authentication of fruit extracts.
Anal Bioanal Chem. 2015 Jan; 407(2):597-608.AB

Abstract

Liquid chromatography-electrospray-tandem mass spectrometry (LC-ESI-MS/MS) was applied to the analysis and authentication of fruit-based products and fruit-based pharmaceutical preparations. A Kinetex C18 reversed-phase column under gradient elution with 0.1 % formic acid aqueous solution and methanol mobile phases was used for the simultaneous determination of 26 polyphenols, allowing an acceptable separation in less than 22 min. Instrumental quality parameters such as limits of detection (LOD, values between 12 and 14 μg/L for 19 of the 26 analyzed polyphenols), linearity (r (2) > 0.991), run-to-run and day-to-day precisions (relative standard deviation (RSD) values lower than 9.9 and 13.5 %, respectively), and accuracy (relative errors lower than 8 %) were established. A simple extraction method, consisting of a sample sonication with acetone/water/hydrochloric acid (70:29.9:0.1 v/v/v) and centrifugation, was proposed. Two calibration procedures, external calibration using standards prepared in water and standard addition, were evaluated for polyphenol quantification in several grape and cranberry fruits and processed fruit products. For a 95 % confidence level, no statistical differences were observed between the two calibration methods (p values between 0.06 and 0.95), denoting that external calibration was suitable enough for the quantitative analysis of polyphenols in fruit-based products. The proposed LC-ESI-MS/MS method was then applied to the analysis of polyphenols in 23 grape-based and cranberry-based natural products and pharmaceutical preparations. Polyphenolic concentration data was then analyzed by principal component analysis (PCA) to extract information of the most significant profile data contributing to authentication of natural extracts according to their fruit of origin.

Authors+Show Affiliations

Department of Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, 08028, Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25370163

Citation

Puigventós, Lidia, et al. "Determination of Polyphenolic Profiles By Liquid Chromatography-electrospray-tandem Mass Spectrometry for the Authentication of Fruit Extracts." Analytical and Bioanalytical Chemistry, vol. 407, no. 2, 2015, pp. 597-608.
Puigventós L, Navarro M, Alechaga É, et al. Determination of polyphenolic profiles by liquid chromatography-electrospray-tandem mass spectrometry for the authentication of fruit extracts. Anal Bioanal Chem. 2015;407(2):597-608.
Puigventós, L., Navarro, M., Alechaga, É., Núñez, O., Saurina, J., Hernández-Cassou, S., & Puignou, L. (2015). Determination of polyphenolic profiles by liquid chromatography-electrospray-tandem mass spectrometry for the authentication of fruit extracts. Analytical and Bioanalytical Chemistry, 407(2), 597-608. https://doi.org/10.1007/s00216-014-8298-2
Puigventós L, et al. Determination of Polyphenolic Profiles By Liquid Chromatography-electrospray-tandem Mass Spectrometry for the Authentication of Fruit Extracts. Anal Bioanal Chem. 2015;407(2):597-608. PubMed PMID: 25370163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of polyphenolic profiles by liquid chromatography-electrospray-tandem mass spectrometry for the authentication of fruit extracts. AU - Puigventós,Lidia, AU - Navarro,Meritxell, AU - Alechaga,Élida, AU - Núñez,Oscar, AU - Saurina,Javier, AU - Hernández-Cassou,Santiago, AU - Puignou,Lluis, Y1 - 2014/11/05/ PY - 2014/09/30/received PY - 2014/10/22/accepted PY - 2014/10/22/revised PY - 2014/11/6/entrez PY - 2014/11/6/pubmed PY - 2015/9/29/medline SP - 597 EP - 608 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 407 IS - 2 N2 - Liquid chromatography-electrospray-tandem mass spectrometry (LC-ESI-MS/MS) was applied to the analysis and authentication of fruit-based products and fruit-based pharmaceutical preparations. A Kinetex C18 reversed-phase column under gradient elution with 0.1 % formic acid aqueous solution and methanol mobile phases was used for the simultaneous determination of 26 polyphenols, allowing an acceptable separation in less than 22 min. Instrumental quality parameters such as limits of detection (LOD, values between 12 and 14 μg/L for 19 of the 26 analyzed polyphenols), linearity (r (2) > 0.991), run-to-run and day-to-day precisions (relative standard deviation (RSD) values lower than 9.9 and 13.5 %, respectively), and accuracy (relative errors lower than 8 %) were established. A simple extraction method, consisting of a sample sonication with acetone/water/hydrochloric acid (70:29.9:0.1 v/v/v) and centrifugation, was proposed. Two calibration procedures, external calibration using standards prepared in water and standard addition, were evaluated for polyphenol quantification in several grape and cranberry fruits and processed fruit products. For a 95 % confidence level, no statistical differences were observed between the two calibration methods (p values between 0.06 and 0.95), denoting that external calibration was suitable enough for the quantitative analysis of polyphenols in fruit-based products. The proposed LC-ESI-MS/MS method was then applied to the analysis of polyphenols in 23 grape-based and cranberry-based natural products and pharmaceutical preparations. Polyphenolic concentration data was then analyzed by principal component analysis (PCA) to extract information of the most significant profile data contributing to authentication of natural extracts according to their fruit of origin. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/25370163/Determination_of_polyphenolic_profiles_by_liquid_chromatography_electrospray_tandem_mass_spectrometry_for_the_authentication_of_fruit_extracts_ L2 - https://dx.doi.org/10.1007/s00216-014-8298-2 DB - PRIME DP - Unbound Medicine ER -