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Phytochemical investigation and anti-microbial activity of Clausena anisata (Willd), Hook.

Abstract

BACKGROUND

Clausena anisata belongs to the family Rutaceae, a shrub widely used in West Africa for the treatment of bacterial and fungal infections of the skin including boils, ringworm and eczema. The study was designed to evaluate the antimicrobial activity and phytochemical screening of ethanol leaf extract of C. anisata (CLE).

METHOD

Antimicrobial activity of CLE was investigated using agar well diffusion and micro-dilution methods against four Gram-positive bacteria (Bacillus substilis NCTC 10073, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Bacillus thuringiensis ATCC 13838) and two Gram-negative bacteria (Pseudomonas aeruginosa ATCC 4853, Proteus vulgaris ATCC 4175) and a clinical isolate of Candida albicans.

RESULTS

CLE was active against all test organisms with minimum inhibitory concentration (MIC), range of 0.5 to 7.0 mg/mL against Gram-positive bacteria, 2.5 to 1.0 mg/mL against Gram-negative bacteria and 5.5mg/mL against C. albicans. The MICs of the methanol fraction of CLE were 0.6 mg to 5.0/mL and 1.0 to 3.0 mg/mL for Gram-positive and Gram-negative bacteria respectively. Chloroform fraction had MIC of 3.0 to 7.5 mg/mL and 2.0 to 6.5 mg/mL for Gram-positive and Gram-negative bacteria, respectively and petroleum ether fraction had 4.5 to 8.0 mg/mL for Gram-positive and Gram-negative bacteria. The CLE exhibited static action against all test organisms within a range of 0.5 to 22.0 mg/mL. Phytochemical screening of C. anisata revealed the presence of tannins, flavonoids, steroids, saponins, glycosides and alkaloids. HPLC finger-printing of the CLE and its fractions were determined.

CONCLUSION

These results may justify the medicinal uses of C. anisata for the treatment of microbial infections.

Authors+Show Affiliations

Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.Forensic Laboratory, Ghana Standards Authority, Accra, Ghana.Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25371583

Citation

Agyepong, Nicholas, et al. "Phytochemical Investigation and Anti-microbial Activity of Clausena Anisata (Willd), Hook." African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, vol. 11, no. 3, 2014, pp. 200-9.
Agyepong N, Agyare C, Adarkwa-Yiadom M, et al. Phytochemical investigation and anti-microbial activity of Clausena anisata (Willd), Hook. Afr J Tradit Complement Altern Med. 2014;11(3):200-9.
Agyepong, N., Agyare, C., Adarkwa-Yiadom, M., & Gbedema, S. Y. (2014). Phytochemical investigation and anti-microbial activity of Clausena anisata (Willd), Hook. African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, 11(3), 200-9.
Agyepong N, et al. Phytochemical Investigation and Anti-microbial Activity of Clausena Anisata (Willd), Hook. Afr J Tradit Complement Altern Med. 2014;11(3):200-9. PubMed PMID: 25371583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical investigation and anti-microbial activity of Clausena anisata (Willd), Hook. AU - Agyepong,Nicholas, AU - Agyare,Christian, AU - Adarkwa-Yiadom,Martin, AU - Gbedema,Stephen Yao, Y1 - 2014/04/03/ PY - 2014/11/6/entrez PY - 2014/11/6/pubmed PY - 2015/7/8/medline KW - Phytochemical screening KW - antibacterial KW - antifungal KW - static action SP - 200 EP - 9 JF - African journal of traditional, complementary, and alternative medicines : AJTCAM JO - Afr J Tradit Complement Altern Med VL - 11 IS - 3 N2 - BACKGROUND: Clausena anisata belongs to the family Rutaceae, a shrub widely used in West Africa for the treatment of bacterial and fungal infections of the skin including boils, ringworm and eczema. The study was designed to evaluate the antimicrobial activity and phytochemical screening of ethanol leaf extract of C. anisata (CLE). METHOD: Antimicrobial activity of CLE was investigated using agar well diffusion and micro-dilution methods against four Gram-positive bacteria (Bacillus substilis NCTC 10073, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Bacillus thuringiensis ATCC 13838) and two Gram-negative bacteria (Pseudomonas aeruginosa ATCC 4853, Proteus vulgaris ATCC 4175) and a clinical isolate of Candida albicans. RESULTS: CLE was active against all test organisms with minimum inhibitory concentration (MIC), range of 0.5 to 7.0 mg/mL against Gram-positive bacteria, 2.5 to 1.0 mg/mL against Gram-negative bacteria and 5.5mg/mL against C. albicans. The MICs of the methanol fraction of CLE were 0.6 mg to 5.0/mL and 1.0 to 3.0 mg/mL for Gram-positive and Gram-negative bacteria respectively. Chloroform fraction had MIC of 3.0 to 7.5 mg/mL and 2.0 to 6.5 mg/mL for Gram-positive and Gram-negative bacteria, respectively and petroleum ether fraction had 4.5 to 8.0 mg/mL for Gram-positive and Gram-negative bacteria. The CLE exhibited static action against all test organisms within a range of 0.5 to 22.0 mg/mL. Phytochemical screening of C. anisata revealed the presence of tannins, flavonoids, steroids, saponins, glycosides and alkaloids. HPLC finger-printing of the CLE and its fractions were determined. CONCLUSION: These results may justify the medicinal uses of C. anisata for the treatment of microbial infections. SN - 2505-0044 UR - https://www.unboundmedicine.com/medline/citation/25371583/Phytochemical_investigation_and_anti_microbial_activity_of_Clausena_anisata__Willd__Hook_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25371583/ DB - PRIME DP - Unbound Medicine ER -