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Immunocytochemical localization of GABAA receptors in goldfish and chicken retinas.
J Comp Neurol 1989; 280(1):15-26JC

Abstract

A monoclonal antibody (mAb 62-3G1) to the GABAA receptor/benzodiazepine receptor/Cl- channel complex from bovine brain was used with light and electron microscopy in goldfish retina and light microscopy in chicken retina to localize GABAA receptor immunoreactivity (GABAr-IR). GABAr-IR was found in the outer plexiform layer (OPL) in both species, in three broad bands in the inner plexiform layer (IPL) of goldfish, and in seven major bands of the chicken IPL. A small percentage of amacrine cell bodies (composing at least three types) were stained in chicken. In goldfish OPL, GABAr-IR was localized intracellularly and along the plasma membrane of cone pedicles, whereas rod spherules were lightly stained, but always only intracellularly. In chicken, all three sublayers of the OPL were GABAr-IR. The presence of GABAr-IR on photoreceptor terminals is consistent with data indicating feedback from GABAergic horizontal cells to cones. In the goldfish IPL, GABAr-IR was localized to postsynaptic sites of amacrine cell synapses; intracellular staining of processes in the IPL also was observed in presumed "GABAergic" targets. A comparison of GABAr-IR with the distributions of 3H-muscimol uptake/binding, glutamate decarboxylase-IR, GABA-IR, and 3H-GABA uptake in the IPL showed either a reasonable correspondence or mismatch, depending on the marker, species, and lamina within the IPL. The distribution of GABAr-IR in the retina corresponded better with the 3H-muscimol than with 3H-benzodiazepine binding patterns yet overall was in excellent agreement with many other physiological and anatomical indicators of GABAergic function. We suggest that intracellular GABAr-IR represents the biosynthetic and/or degradative pathway of the receptor and we conclude that mAb 62-3G1 is a valid marker of GABAA receptors in these retinas and will serve as a useful probe with which to address the issue of mismatches between the localization of GABAA receptors and indicators of presynaptic GABAergic terminals.

Authors+Show Affiliations

Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2537342

Citation

Yazulla, S, et al. "Immunocytochemical Localization of GABAA Receptors in Goldfish and Chicken Retinas." The Journal of Comparative Neurology, vol. 280, no. 1, 1989, pp. 15-26.
Yazulla S, Studholme KM, Vitorica J, et al. Immunocytochemical localization of GABAA receptors in goldfish and chicken retinas. J Comp Neurol. 1989;280(1):15-26.
Yazulla, S., Studholme, K. M., Vitorica, J., & de Blas, A. L. (1989). Immunocytochemical localization of GABAA receptors in goldfish and chicken retinas. The Journal of Comparative Neurology, 280(1), pp. 15-26.
Yazulla S, et al. Immunocytochemical Localization of GABAA Receptors in Goldfish and Chicken Retinas. J Comp Neurol. 1989 Feb 1;280(1):15-26. PubMed PMID: 2537342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunocytochemical localization of GABAA receptors in goldfish and chicken retinas. AU - Yazulla,S, AU - Studholme,K M, AU - Vitorica,J, AU - de Blas,A L, PY - 1989/2/1/pubmed PY - 1989/2/1/medline PY - 1989/2/1/entrez SP - 15 EP - 26 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 280 IS - 1 N2 - A monoclonal antibody (mAb 62-3G1) to the GABAA receptor/benzodiazepine receptor/Cl- channel complex from bovine brain was used with light and electron microscopy in goldfish retina and light microscopy in chicken retina to localize GABAA receptor immunoreactivity (GABAr-IR). GABAr-IR was found in the outer plexiform layer (OPL) in both species, in three broad bands in the inner plexiform layer (IPL) of goldfish, and in seven major bands of the chicken IPL. A small percentage of amacrine cell bodies (composing at least three types) were stained in chicken. In goldfish OPL, GABAr-IR was localized intracellularly and along the plasma membrane of cone pedicles, whereas rod spherules were lightly stained, but always only intracellularly. In chicken, all three sublayers of the OPL were GABAr-IR. The presence of GABAr-IR on photoreceptor terminals is consistent with data indicating feedback from GABAergic horizontal cells to cones. In the goldfish IPL, GABAr-IR was localized to postsynaptic sites of amacrine cell synapses; intracellular staining of processes in the IPL also was observed in presumed "GABAergic" targets. A comparison of GABAr-IR with the distributions of 3H-muscimol uptake/binding, glutamate decarboxylase-IR, GABA-IR, and 3H-GABA uptake in the IPL showed either a reasonable correspondence or mismatch, depending on the marker, species, and lamina within the IPL. The distribution of GABAr-IR in the retina corresponded better with the 3H-muscimol than with 3H-benzodiazepine binding patterns yet overall was in excellent agreement with many other physiological and anatomical indicators of GABAergic function. We suggest that intracellular GABAr-IR represents the biosynthetic and/or degradative pathway of the receptor and we conclude that mAb 62-3G1 is a valid marker of GABAA receptors in these retinas and will serve as a useful probe with which to address the issue of mismatches between the localization of GABAA receptors and indicators of presynaptic GABAergic terminals. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/2537342/Immunocytochemical_localization_of_GABAA_receptors_in_goldfish_and_chicken_retinas_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=1989&volume=280&issue=1&spage=15 DB - PRIME DP - Unbound Medicine ER -