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Activity of hepatic galactose-metabolizing enzymes in the pregnant rat and fetus.
Pediatr Res. 1989 Feb; 25(2):161-6.PR

Abstract

The sp act of hepatic galactose-metabolizing enzymes, galactokinase, galactose-1-phosphate uridyltransferase, and uridine diphosphate-4-epimerase were measured in female rats during pregnancy and lactation as well as in fetuses and pups after parturition. Sp act for transferase and epimerase in pregnant rat liver are about 50% higher than that of virgin females, and with the increase in organ size during pregnancy the total hepatic activity is double that of nonpregnant animals. Galactokinase activity decreases somewhat during pregnancy, but total activity is 25% higher than in virgin liver. A Michaelis-Menten kinetic analysis of liver transferase indicates an increase in the maximum velocity of the reaction without a change in Km. Isoelectricfocusing on a high-resolution IEF gel demonstrated similar isozyme patterns. The sp act of the fetal liver enzymes increase to about twice that of the maternal tissue, but total activities are low due to the very small fetal liver size. Sp act of these enzymes in maternal liver fall after delivery, but sp act of galactokinase and transferase are programmed to increase in liver of the growing neonatal animals, reaching levels almost 5-fold higher than found in nonpregnant adult liver. An understanding of factors contributing to the enhanced transferase activity of the liver of pregnant and neonatal rats may contribute to possible ways of augmenting the residual transferase activity of patients with transferase-deficient galactosemia as a therapeutic strategy.

Authors+Show Affiliations

Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, Pennsylvania 19104.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2537489

Citation

Rogers, S R., et al. "Activity of Hepatic Galactose-metabolizing Enzymes in the Pregnant Rat and Fetus." Pediatric Research, vol. 25, no. 2, 1989, pp. 161-6.
Rogers SR, Bovee BW, Saunders SL, et al. Activity of hepatic galactose-metabolizing enzymes in the pregnant rat and fetus. Pediatr Res. 1989;25(2):161-6.
Rogers, S. R., Bovee, B. W., Saunders, S. L., & Segal, S. (1989). Activity of hepatic galactose-metabolizing enzymes in the pregnant rat and fetus. Pediatric Research, 25(2), 161-6.
Rogers SR, et al. Activity of Hepatic Galactose-metabolizing Enzymes in the Pregnant Rat and Fetus. Pediatr Res. 1989;25(2):161-6. PubMed PMID: 2537489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activity of hepatic galactose-metabolizing enzymes in the pregnant rat and fetus. AU - Rogers,S R, AU - Bovee,B W, AU - Saunders,S L, AU - Segal,S, PY - 1989/2/1/pubmed PY - 1989/2/1/medline PY - 1989/2/1/entrez SP - 161 EP - 6 JF - Pediatric research JO - Pediatr Res VL - 25 IS - 2 N2 - The sp act of hepatic galactose-metabolizing enzymes, galactokinase, galactose-1-phosphate uridyltransferase, and uridine diphosphate-4-epimerase were measured in female rats during pregnancy and lactation as well as in fetuses and pups after parturition. Sp act for transferase and epimerase in pregnant rat liver are about 50% higher than that of virgin females, and with the increase in organ size during pregnancy the total hepatic activity is double that of nonpregnant animals. Galactokinase activity decreases somewhat during pregnancy, but total activity is 25% higher than in virgin liver. A Michaelis-Menten kinetic analysis of liver transferase indicates an increase in the maximum velocity of the reaction without a change in Km. Isoelectricfocusing on a high-resolution IEF gel demonstrated similar isozyme patterns. The sp act of the fetal liver enzymes increase to about twice that of the maternal tissue, but total activities are low due to the very small fetal liver size. Sp act of these enzymes in maternal liver fall after delivery, but sp act of galactokinase and transferase are programmed to increase in liver of the growing neonatal animals, reaching levels almost 5-fold higher than found in nonpregnant adult liver. An understanding of factors contributing to the enhanced transferase activity of the liver of pregnant and neonatal rats may contribute to possible ways of augmenting the residual transferase activity of patients with transferase-deficient galactosemia as a therapeutic strategy. SN - 0031-3998 UR - https://www.unboundmedicine.com/medline/citation/2537489/Activity_of_hepatic_galactose_metabolizing_enzymes_in_the_pregnant_rat_and_fetus_ L2 - https://doi.org/10.1203/00006450-198902000-00017 DB - PRIME DP - Unbound Medicine ER -