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Prevention of Selenite-Induced Cataratogenesis by Ginkgo biloba Extract (Egb761) in Wistar Rats.
Curr Eye Res. 2015; 40(10):1028-33.CE

Abstract

OBJECTIVE

To investigate whether Ginkgo biloba extract retards selenite-induced cataractogenesis in Wistar rat pups.

METHODS

On postpartum day eight, Group I rat pups (n = 12) received an intraperitoneal injection of physiological saline. Groups II and III rat pups (n = 12) received a subcutaneous injection of sodium selenite. Group III also received an intraperitoneal injection of G. biloba extract once daily on postpartum days 9-14. Both eyes of each pup were examined from postpartum day 16 up to day 30. After sacrifice, encapsulated pup lens were analyzed for mean activities of catalase, superoxide dismutase, glutathione peroxidase (GPx), glutathione S-transferase and glutathione reductase. In addition, the mean concentrations of reduced glutathione (GSH) and malondialdehyde were analyzed in samples of lens and serum.

RESULTS

Dense lenticular opacification occurs 100% in Group II, but only minimal opacification occurs in three pups of Group III (25%), no opacification in 75% of Group III, none in Group I. Compared with Groups I and III, Group II rat showed lower lenticular antioxidant enzyme activity, lower level of GSH, and higher level of malondidehyde (mean ± standard deviation SD, p < 0.05 respectively).

CONCLUSIONS

The treatment with G. biloba extract is effective against oxidative stress - a crucial factor of cataractogenesis in rat pups, possibly by preventing depletion of antioxidant enzymes and by inhibiting lipid peroxidation.

Authors+Show Affiliations

a Beijing Tong Ren Hospital, Capital Medical University , Beijing , People's Republic of China . b Capital Medical University School of Rehabilitation Medicine, China Rehabilitation Research Center , Beijing , People's Republic of China and.c Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing , People's Republic of China.a Beijing Tong Ren Hospital, Capital Medical University , Beijing , People's Republic of China .b Capital Medical University School of Rehabilitation Medicine, China Rehabilitation Research Center , Beijing , People's Republic of China and.c Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing , People's Republic of China.b Capital Medical University School of Rehabilitation Medicine, China Rehabilitation Research Center , Beijing , People's Republic of China and.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25380229

Citation

Cao, Shuqin, et al. "Prevention of Selenite-Induced Cataratogenesis By Ginkgo Biloba Extract (Egb761) in Wistar Rats." Current Eye Research, vol. 40, no. 10, 2015, pp. 1028-33.
Cao S, Gao M, Wang N, et al. Prevention of Selenite-Induced Cataratogenesis by Ginkgo biloba Extract (Egb761) in Wistar Rats. Curr Eye Res. 2015;40(10):1028-33.
Cao, S., Gao, M., Wang, N., Liu, N., Du, G., & Lu, J. (2015). Prevention of Selenite-Induced Cataratogenesis by Ginkgo biloba Extract (Egb761) in Wistar Rats. Current Eye Research, 40(10), 1028-33. https://doi.org/10.3109/02713683.2014.980005
Cao S, et al. Prevention of Selenite-Induced Cataratogenesis By Ginkgo Biloba Extract (Egb761) in Wistar Rats. Curr Eye Res. 2015;40(10):1028-33. PubMed PMID: 25380229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevention of Selenite-Induced Cataratogenesis by Ginkgo biloba Extract (Egb761) in Wistar Rats. AU - Cao,Shuqin, AU - Gao,Mei, AU - Wang,Ningli, AU - Liu,Na, AU - Du,Guanhua, AU - Lu,Jianmin, Y1 - 2014/11/07/ PY - 2014/11/8/entrez PY - 2014/11/8/pubmed PY - 2016/4/16/medline KW - Antioxidant enzymes KW - Ginkgo biloba extract KW - cataract KW - free radicals KW - oxidative stress KW - selenite SP - 1028 EP - 33 JF - Current eye research JO - Curr Eye Res VL - 40 IS - 10 N2 - OBJECTIVE: To investigate whether Ginkgo biloba extract retards selenite-induced cataractogenesis in Wistar rat pups. METHODS: On postpartum day eight, Group I rat pups (n = 12) received an intraperitoneal injection of physiological saline. Groups II and III rat pups (n = 12) received a subcutaneous injection of sodium selenite. Group III also received an intraperitoneal injection of G. biloba extract once daily on postpartum days 9-14. Both eyes of each pup were examined from postpartum day 16 up to day 30. After sacrifice, encapsulated pup lens were analyzed for mean activities of catalase, superoxide dismutase, glutathione peroxidase (GPx), glutathione S-transferase and glutathione reductase. In addition, the mean concentrations of reduced glutathione (GSH) and malondialdehyde were analyzed in samples of lens and serum. RESULTS: Dense lenticular opacification occurs 100% in Group II, but only minimal opacification occurs in three pups of Group III (25%), no opacification in 75% of Group III, none in Group I. Compared with Groups I and III, Group II rat showed lower lenticular antioxidant enzyme activity, lower level of GSH, and higher level of malondidehyde (mean ± standard deviation SD, p < 0.05 respectively). CONCLUSIONS: The treatment with G. biloba extract is effective against oxidative stress - a crucial factor of cataractogenesis in rat pups, possibly by preventing depletion of antioxidant enzymes and by inhibiting lipid peroxidation. SN - 1460-2202 UR - https://www.unboundmedicine.com/medline/citation/25380229/Prevention_of_Selenite_Induced_Cataratogenesis_by_Ginkgo_biloba_Extract__Egb761__in_Wistar_Rats_ L2 - https://www.tandfonline.com/doi/full/10.3109/02713683.2014.980005 DB - PRIME DP - Unbound Medicine ER -