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In vitro α-glucosidase inhibition, antioxidant, anticancer, and antimycobacterial properties of ethyl acetate extract of Aegle tamilnadensis Abdul Kader (Rutaceae) leaf.
Appl Biochem Biotechnol. 2015 Jan; 175(2):1247-61.AB

Abstract

The present study was aimed to investigate in vitro α-glucosidase inhibition, antioxidant, anticancer, and antimycobacterial activities of the ethyl acetate extract of A. tamilnadensis leaves. The extract recorded strong α-glucosidase inhibition with an IC50 value of 100 μg/ml. The antioxidant potential of the extract was evaluated by nitric oxide radical inhibition, lipid peroxidation inhibition, ferric thiocyanate, and ABTS radical scavenging assay, and the extract recorded significant antioxidant activity. The ferric thiocyanate activity of extract was superior to butylated hydroxyl anisol (BHA), the standard antioxidant agent. The anticancer activity of the extract was evaluated against (1) breast cancer cell lines (MDAM B-231), (2) cervical cancer cell lines (HeLa), and (3) lung cancer cell line (A 549) using MTT assay, and significant activity was recorded against A 549 with an IC50 value of 64 μg/ml. Further studies on the morphology, acridine orange/ethidium bromide staining, and cell cycle analysis by flow cytometry confirm the extract-induced apoptosis in A 549. This extract also recorded significant anti-tuberculosis activity against Mycobacterium smegmatis. The current study suggests that the ethyl acetate extract of A. tamilnadensis is a potential source of natural α-glucosidase inhibitor and antioxidant for protection as well as prevention of life-threatening diseases like cancer.

Authors+Show Affiliations

Department of Biotechnology, S.D.V. College of Arts and Applied Science, Sanathanapuram P.O. Kalarcode, Alappuzha District, Kerala, 688003, India, drpratapchandran@yahoo.co.in.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25380641

Citation

R, Pratap Chandran, et al. "In Vitro Α-glucosidase Inhibition, Antioxidant, Anticancer, and Antimycobacterial Properties of Ethyl Acetate Extract of Aegle Tamilnadensis Abdul Kader (Rutaceae) Leaf." Applied Biochemistry and Biotechnology, vol. 175, no. 2, 2015, pp. 1247-61.
R PC, S NK, S M, et al. In vitro α-glucosidase inhibition, antioxidant, anticancer, and antimycobacterial properties of ethyl acetate extract of Aegle tamilnadensis Abdul Kader (Rutaceae) leaf. Appl Biochem Biotechnol. 2015;175(2):1247-61.
R, P. C., S, N. K., S, M., S, A. K., & B S, D. K. (2015). In vitro α-glucosidase inhibition, antioxidant, anticancer, and antimycobacterial properties of ethyl acetate extract of Aegle tamilnadensis Abdul Kader (Rutaceae) leaf. Applied Biochemistry and Biotechnology, 175(2), 1247-61. https://doi.org/10.1007/s12010-014-1335-y
R PC, et al. In Vitro Α-glucosidase Inhibition, Antioxidant, Anticancer, and Antimycobacterial Properties of Ethyl Acetate Extract of Aegle Tamilnadensis Abdul Kader (Rutaceae) Leaf. Appl Biochem Biotechnol. 2015;175(2):1247-61. PubMed PMID: 25380641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro α-glucosidase inhibition, antioxidant, anticancer, and antimycobacterial properties of ethyl acetate extract of Aegle tamilnadensis Abdul Kader (Rutaceae) leaf. AU - R,Pratap Chandran, AU - S,Nishanth Kumar, AU - S,Manju, AU - S,Abdul Kader, AU - B S,Dileep Kumar, Y1 - 2014/11/08/ PY - 2014/05/07/received PY - 2014/10/15/accepted PY - 2014/11/9/entrez PY - 2014/11/9/pubmed PY - 2015/9/12/medline SP - 1247 EP - 61 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 175 IS - 2 N2 - The present study was aimed to investigate in vitro α-glucosidase inhibition, antioxidant, anticancer, and antimycobacterial activities of the ethyl acetate extract of A. tamilnadensis leaves. The extract recorded strong α-glucosidase inhibition with an IC50 value of 100 μg/ml. The antioxidant potential of the extract was evaluated by nitric oxide radical inhibition, lipid peroxidation inhibition, ferric thiocyanate, and ABTS radical scavenging assay, and the extract recorded significant antioxidant activity. The ferric thiocyanate activity of extract was superior to butylated hydroxyl anisol (BHA), the standard antioxidant agent. The anticancer activity of the extract was evaluated against (1) breast cancer cell lines (MDAM B-231), (2) cervical cancer cell lines (HeLa), and (3) lung cancer cell line (A 549) using MTT assay, and significant activity was recorded against A 549 with an IC50 value of 64 μg/ml. Further studies on the morphology, acridine orange/ethidium bromide staining, and cell cycle analysis by flow cytometry confirm the extract-induced apoptosis in A 549. This extract also recorded significant anti-tuberculosis activity against Mycobacterium smegmatis. The current study suggests that the ethyl acetate extract of A. tamilnadensis is a potential source of natural α-glucosidase inhibitor and antioxidant for protection as well as prevention of life-threatening diseases like cancer. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/25380641/In_vitro_α_glucosidase_inhibition_antioxidant_anticancer_and_antimycobacterial_properties_of_ethyl_acetate_extract_of_Aegle_tamilnadensis_Abdul_Kader__Rutaceae__leaf_ DB - PRIME DP - Unbound Medicine ER -