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Antioxidant activity characterization, phytochemical screening, and proximate analysis of Cermela Hutan (Phyllanthus gomphocarpus Hook. F) roots and leaves.
Med Sci Monit Basic Res. 2014 Nov 07; 20:170-5.MS

Abstract

BACKGROUND

Roots and leaves of the Cermela Hutan (Phyllanthus gomphocarpus Hook. F) plant were studied to determine antioxidant activity, phytochemical compounds, proportion of carbohydrate, crude protein, moisture, ash, fat, total phenolic content (TPC), and total flavonoid content (TFC).

MATERIAL/METHODS

Ten percent (10%) aqueous extract from both Phyllanthus gomphocarpus roots (PGR) and leaves (PGL) were used in this study. Antioxidant activity characterization by TPC, TFC, Ferric Reducing Antioxidant Power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and phytochemical screening, as well as proximate analysis from both extracts were analyzed in this study.

RESULTS

Phyllanthus gomphocarpus roots (PGR) and leaves (PGL) tested positive for flavonoid, saponin, tannins, and terpenoids, but PGR showed negative result for anthraquinones. In average weight of 100.0 g dry sample, the carbohydrates, protein, moisture, ash, fat, and energy content in PGR and PGL were 80.9%, 5.5%, 7.8%, 3.4%, 2.4%, and 367 Kcal/100g, and 66.5%, 14.8%, 10.7%, 6.5%, 1.5%, and 399 Kcal/100 g, respectively. Antioxidant assessments using FRAP and DPPH assay showed that PGL extracts possessed higher antioxidant capacity by reducing the ferric ion-TPTZ complex by 0.14 mg/ml ±0.0018 and higher scavenging activity, 83.83% ±0.54 as compared to PGR, 0.07 mg/ml ±0.0035 for FRAP and 62.87% ±1.33 for DPPH, respectively. The total phenolics content was significantly higher in PGL (208.77 mg GAE/g ±3.79) as compared to PGR (27.53 mg GAE/g ±0.42). However, there was no significant different in the total flavonoid contents for PGR (34.8 mg QE/g ±3.12) and PGL (32.43 mg QE/g ±3.92).

CONCLUSIONS

Further investigations are suggested to isolate and characterize the other active constituents from this plant in combatting diseases.

Authors+Show Affiliations

Natural Product & Phytomedicine Biotechnology Research Laboratory (NPPBRL), Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam, Selangor, Malaysia.Natural Product & Phytomedicine Biotechnology Research Laboratory (NPPBRL), Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam, Selangor, Malaysia.Department of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam, Selangor, Malaysia.Department of Basic Sciences, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam, Selangor, Malaysia.Department of Basic Sciences, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam, Selangor, Malaysia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25381551

Citation

Bahari, Ebby-Anuar, et al. "Antioxidant Activity Characterization, Phytochemical Screening, and Proximate Analysis of Cermela Hutan (Phyllanthus Gomphocarpus Hook. F) Roots and Leaves." Medical Science Monitor Basic Research, vol. 20, 2014, pp. 170-5.
Bahari EA, Zaaba NE, Haron N, et al. Antioxidant activity characterization, phytochemical screening, and proximate analysis of Cermela Hutan (Phyllanthus gomphocarpus Hook. F) roots and leaves. Med Sci Monit Basic Res. 2014;20:170-5.
Bahari, E. A., Zaaba, N. E., Haron, N., Dasiman, R., & Amom, Z. (2014). Antioxidant activity characterization, phytochemical screening, and proximate analysis of Cermela Hutan (Phyllanthus gomphocarpus Hook. F) roots and leaves. Medical Science Monitor Basic Research, 20, 170-5. https://doi.org/10.12659/MSMBR.892345
Bahari EA, et al. Antioxidant Activity Characterization, Phytochemical Screening, and Proximate Analysis of Cermela Hutan (Phyllanthus Gomphocarpus Hook. F) Roots and Leaves. Med Sci Monit Basic Res. 2014 Nov 7;20:170-5. PubMed PMID: 25381551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity characterization, phytochemical screening, and proximate analysis of Cermela Hutan (Phyllanthus gomphocarpus Hook. F) roots and leaves. AU - Bahari,Ebby-Anuar, AU - Zaaba,Nur Eleza, AU - Haron,Norhisham, AU - Dasiman,Razif, AU - Amom,Zulkhairi, Y1 - 2014/11/07/ PY - 2014/11/10/entrez PY - 2014/11/10/pubmed PY - 2015/2/24/medline SP - 170 EP - 5 JF - Medical science monitor basic research JO - Med Sci Monit Basic Res VL - 20 N2 - BACKGROUND: Roots and leaves of the Cermela Hutan (Phyllanthus gomphocarpus Hook. F) plant were studied to determine antioxidant activity, phytochemical compounds, proportion of carbohydrate, crude protein, moisture, ash, fat, total phenolic content (TPC), and total flavonoid content (TFC). MATERIAL/METHODS: Ten percent (10%) aqueous extract from both Phyllanthus gomphocarpus roots (PGR) and leaves (PGL) were used in this study. Antioxidant activity characterization by TPC, TFC, Ferric Reducing Antioxidant Power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and phytochemical screening, as well as proximate analysis from both extracts were analyzed in this study. RESULTS: Phyllanthus gomphocarpus roots (PGR) and leaves (PGL) tested positive for flavonoid, saponin, tannins, and terpenoids, but PGR showed negative result for anthraquinones. In average weight of 100.0 g dry sample, the carbohydrates, protein, moisture, ash, fat, and energy content in PGR and PGL were 80.9%, 5.5%, 7.8%, 3.4%, 2.4%, and 367 Kcal/100g, and 66.5%, 14.8%, 10.7%, 6.5%, 1.5%, and 399 Kcal/100 g, respectively. Antioxidant assessments using FRAP and DPPH assay showed that PGL extracts possessed higher antioxidant capacity by reducing the ferric ion-TPTZ complex by 0.14 mg/ml ±0.0018 and higher scavenging activity, 83.83% ±0.54 as compared to PGR, 0.07 mg/ml ±0.0035 for FRAP and 62.87% ±1.33 for DPPH, respectively. The total phenolics content was significantly higher in PGL (208.77 mg GAE/g ±3.79) as compared to PGR (27.53 mg GAE/g ±0.42). However, there was no significant different in the total flavonoid contents for PGR (34.8 mg QE/g ±3.12) and PGL (32.43 mg QE/g ±3.92). CONCLUSIONS: Further investigations are suggested to isolate and characterize the other active constituents from this plant in combatting diseases. SN - 2325-4416 UR - https://www.unboundmedicine.com/medline/citation/25381551/Antioxidant_activity_characterization_phytochemical_screening_and_proximate_analysis_of_Cermela_Hutan__Phyllanthus_gomphocarpus_Hook__F__roots_and_leaves_ L2 - https://basic.medscimonit.com/download/index/idArt/892345 DB - PRIME DP - Unbound Medicine ER -