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Antibacterial Mode of Action of Cinnamomum verum Bark Essential Oil, Alone and in Combination with Piperacillin, Against a Multi-Drug-Resistant Escherichia coli Strain.
J Microbiol Biotechnol. 2015 Aug; 25(8):1299-306.JM

Abstract

This study aimed to investigate the mechanism of action of the cinnamon bark essential oil (CB), when used singly and also in combination with piperacillin, for its antimicrobial and synergistic activity against beta-lactamase TEM-1 plasmid-conferred Escherichia coli J53 R1. Viable count of this combination showed a complete killing profile at 20 h and further confirmed its synergistic effect by reducing the bacteria cell numbers. Analysis on the stability of treated cultures for cell membrane permeability by CB when tested against sodium dodecyl sulfate revealed that the bacterial cell membrane was disrupted by the essential oils. Scanning electron microscopy observation and bacterial surface charge measurement also revealed that CB causes irreversible membrane damage and reduces the bacterial surface charge. In addition, bioluminescence expression of Escherichia coli [pSB1075] and E. coli [pSB401] by CB showed reduction, indicating the possibility of the presence of quorum sensing (QS) inhibitors. Gas-chromatography and mass spectrometry of the essential oil of Cinnamomum verum showed that trans-cinnamaldehyde (72.81%), benzyl alcohol (12.5%), and eugenol (6.57%) were the major components in the essential oil. From this study, CB has the potential to reverse E. coli J53 R1 resistance to piperacillin through two pathways; modification in the permeability of the outer membrane or bacterial QS inhibition.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Yap PS
School of Postgraduate Studies and Research, International Medical University, 57000 Kuala Lumpur, Malaysia.
Krishnan T
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Chan KG
Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Lim SH
Department of Physiology, Perdana University-Royal College of Surgeons Ireland (PU-RCSI), 43400 Serdang, Selangor, Malaysia.

MeSH

Anti-Bacterial AgentsCell MembraneCinnamomum zeylanicumColony Count, MicrobialDrug Resistance, Multiple, BacterialDrug SynergismEscherichia coliGas Chromatography-Mass SpectrometryMicrobial ViabilityMicroscopy, Electron, ScanningOils, VolatilePiperacillinPlant BarkPlasmidsQuorum Sensingbeta-Lactamases

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25381741

Citation

Yap, Polly Soo Xi, et al. "Antibacterial Mode of Action of Cinnamomum Verum Bark Essential Oil, Alone and in Combination With Piperacillin, Against a Multi-Drug-Resistant Escherichia Coli Strain." Journal of Microbiology and Biotechnology, vol. 25, no. 8, 2015, pp. 1299-306.
Yap PS, Krishnan T, Chan KG, et al. Antibacterial Mode of Action of Cinnamomum verum Bark Essential Oil, Alone and in Combination with Piperacillin, Against a Multi-Drug-Resistant Escherichia coli Strain. J Microbiol Biotechnol. 2015;25(8):1299-306.
Yap, P. S., Krishnan, T., Chan, K. G., & Lim, S. H. (2015). Antibacterial Mode of Action of Cinnamomum verum Bark Essential Oil, Alone and in Combination with Piperacillin, Against a Multi-Drug-Resistant Escherichia coli Strain. Journal of Microbiology and Biotechnology, 25(8), 1299-306. https://doi.org/10.4014/jmb.1407.07054
Yap PS, et al. Antibacterial Mode of Action of Cinnamomum Verum Bark Essential Oil, Alone and in Combination With Piperacillin, Against a Multi-Drug-Resistant Escherichia Coli Strain. J Microbiol Biotechnol. 2015;25(8):1299-306. PubMed PMID: 25381741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial Mode of Action of Cinnamomum verum Bark Essential Oil, Alone and in Combination with Piperacillin, Against a Multi-Drug-Resistant Escherichia coli Strain. AU - Yap,Polly Soo Xi, AU - Krishnan,Thiba, AU - Chan,Kok-Gan, AU - Lim,Swee Hua Erin, PY - 2014/11/11/entrez PY - 2014/11/11/pubmed PY - 2016/5/14/medline KW - Cinnamomum verum KW - antibacterial activity KW - chemical composition KW - essential oil KW - membrane permeability KW - quorum sensing SP - 1299 EP - 306 JF - Journal of microbiology and biotechnology JO - J Microbiol Biotechnol VL - 25 IS - 8 N2 - This study aimed to investigate the mechanism of action of the cinnamon bark essential oil (CB), when used singly and also in combination with piperacillin, for its antimicrobial and synergistic activity against beta-lactamase TEM-1 plasmid-conferred Escherichia coli J53 R1. Viable count of this combination showed a complete killing profile at 20 h and further confirmed its synergistic effect by reducing the bacteria cell numbers. Analysis on the stability of treated cultures for cell membrane permeability by CB when tested against sodium dodecyl sulfate revealed that the bacterial cell membrane was disrupted by the essential oils. Scanning electron microscopy observation and bacterial surface charge measurement also revealed that CB causes irreversible membrane damage and reduces the bacterial surface charge. In addition, bioluminescence expression of Escherichia coli [pSB1075] and E. coli [pSB401] by CB showed reduction, indicating the possibility of the presence of quorum sensing (QS) inhibitors. Gas-chromatography and mass spectrometry of the essential oil of Cinnamomum verum showed that trans-cinnamaldehyde (72.81%), benzyl alcohol (12.5%), and eugenol (6.57%) were the major components in the essential oil. From this study, CB has the potential to reverse E. coli J53 R1 resistance to piperacillin through two pathways; modification in the permeability of the outer membrane or bacterial QS inhibition. SN - 1738-8872 UR - https://www.unboundmedicine.com/medline/citation/25381741/Antibacterial_Mode_of_Action_of_Cinnamomum_verum_Bark_Essential_Oil_Alone_and_in_Combination_with_Piperacillin_Against_a_Multi_Drug_Resistant_Escherichia_coli_Strain_ DB - PRIME DP - Unbound Medicine ER -