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Pine bark and green tea concentrated extracts: antioxidant activity and comprehensive characterization of bioactive compounds by HPLC-ESI-QTOF-MS.
Int J Mol Sci. 2014 Nov 06; 15(11):20382-402.IJ

Abstract

The consumption of polyphenols has frequently been associated with low incidence of degenerative diseases. Most of these natural antioxidants come from fruits, vegetables, spices, grains and herbs. For this reason, there has been increasing interest in identifying plant extract compounds. Polymeric tannins and monomeric flavonoids, such as catechin and epicatechin, in pine bark and green tea extracts could be responsible for the higher antioxidant activities of these extracts. The aim of the present study was to characterize the phenolic compounds in pine bark and green tea concentrated extracts using high-performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-QTOF-MS). A total of 37 and 35 compounds from pine bark and green tea extracts, respectively, were identified as belonging to various structural classes, mainly flavan-3-ol and its derivatives (including procyanidins). The antioxidant capacity of both extracts was evaluated by three complementary antioxidant activity methods: Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC). Higher antioxidant activity values by each method were obtained. In addition, total polyphenol and flavan-3-ol contents, which were determined by Folin-Ciocalteu and vanillin assays, respectively, exhibited higher amounts of gallic acid and (+)-catechin equivalents.

Authors+Show Affiliations

Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, 18071 Granada, Spain. mluzcadiz@ugr.es.Biomedical Research Centre, University Hospital of Sant Joan, IISPV, Rovira i Virgili University, C/Sant Joan s/n, 43201 Reus (Tarragona), Spain. sfernandez@fiispv.cat.Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, 18071 Granada, Spain. ansegura@ugr.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25383680

Citation

de la Luz Cádiz-Gurrea, María, et al. "Pine Bark and Green Tea Concentrated Extracts: Antioxidant Activity and Comprehensive Characterization of Bioactive Compounds By HPLC-ESI-QTOF-MS." International Journal of Molecular Sciences, vol. 15, no. 11, 2014, pp. 20382-402.
de la Luz Cádiz-Gurrea M, Fernández-Arroyo S, Segura-Carretero A. Pine bark and green tea concentrated extracts: antioxidant activity and comprehensive characterization of bioactive compounds by HPLC-ESI-QTOF-MS. Int J Mol Sci. 2014;15(11):20382-402.
de la Luz Cádiz-Gurrea, M., Fernández-Arroyo, S., & Segura-Carretero, A. (2014). Pine bark and green tea concentrated extracts: antioxidant activity and comprehensive characterization of bioactive compounds by HPLC-ESI-QTOF-MS. International Journal of Molecular Sciences, 15(11), 20382-402. https://doi.org/10.3390/ijms151120382
de la Luz Cádiz-Gurrea M, Fernández-Arroyo S, Segura-Carretero A. Pine Bark and Green Tea Concentrated Extracts: Antioxidant Activity and Comprehensive Characterization of Bioactive Compounds By HPLC-ESI-QTOF-MS. Int J Mol Sci. 2014 Nov 6;15(11):20382-402. PubMed PMID: 25383680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pine bark and green tea concentrated extracts: antioxidant activity and comprehensive characterization of bioactive compounds by HPLC-ESI-QTOF-MS. AU - de la Luz Cádiz-Gurrea,María, AU - Fernández-Arroyo,Salvador, AU - Segura-Carretero,Antonio, Y1 - 2014/11/06/ PY - 2014/07/28/received PY - 2014/09/24/revised PY - 2014/10/28/accepted PY - 2014/11/11/entrez PY - 2014/11/11/pubmed PY - 2015/6/26/medline SP - 20382 EP - 402 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 15 IS - 11 N2 - The consumption of polyphenols has frequently been associated with low incidence of degenerative diseases. Most of these natural antioxidants come from fruits, vegetables, spices, grains and herbs. For this reason, there has been increasing interest in identifying plant extract compounds. Polymeric tannins and monomeric flavonoids, such as catechin and epicatechin, in pine bark and green tea extracts could be responsible for the higher antioxidant activities of these extracts. The aim of the present study was to characterize the phenolic compounds in pine bark and green tea concentrated extracts using high-performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-QTOF-MS). A total of 37 and 35 compounds from pine bark and green tea extracts, respectively, were identified as belonging to various structural classes, mainly flavan-3-ol and its derivatives (including procyanidins). The antioxidant capacity of both extracts was evaluated by three complementary antioxidant activity methods: Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC). Higher antioxidant activity values by each method were obtained. In addition, total polyphenol and flavan-3-ol contents, which were determined by Folin-Ciocalteu and vanillin assays, respectively, exhibited higher amounts of gallic acid and (+)-catechin equivalents. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/25383680/Pine_bark_and_green_tea_concentrated_extracts:_antioxidant_activity_and_comprehensive_characterization_of_bioactive_compounds_by_HPLC_ESI_QTOF_MS_ L2 - http://www.mdpi.com/resolver?pii=ijms151120382 DB - PRIME DP - Unbound Medicine ER -