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Comparative genomic sequence analysis of Chinese orf virus strain NA1/11 with other parapoxviruses.
Arch Virol 2015; 160(1):253-66AV

Abstract

Orf virus (ORFV) is a typical member of the genus Parapoxvirus. The parapoxvirus genome consists of highly variable terminal regions and relatively conserved central regions with a high G + C content. In our previous study, a novel ORFV strain, NA1/11, was isolated from northeastern China. To fully characterize this strain, we sequenced the entire genome of NA1/11 and conducted a comparative analysis using multiple parapoxviruses. The genomic sequence of NA1/11 was found to consist of 137,080 nucleotides with a G + C content of 63.6 %, but it did not contain the terminal hairpin sequence. Alignment of ORFs from NA1/11 with NZ2, IA82 and SA00 revealed several highly variable ORFs, while the most evident ones are ORFs 001, 103, 109-110, 116 and 132. An odd phenomenon in the region of ORFs 118-120 is that the non-coding fragments are almost as long as the coding fragments. By comparative analysis of inverted terminal repeats, we identified one repeat motif and a long conserved fragment. By comparing the ITRs of SA00 with those of three other ORFVs, more clues were obtained about the correlation between ITR sequence and host adaption. Comparison of the NA1/11 genome with the sequences of other strains of ORFV revealed highly variable regions, thus providing new insights into the genetic diversity of ORFV.

Authors+Show Affiliations

Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, 1838 N. Guangzhou Avenue, Guangzhou, 510515, Guangdong, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25385177

Citation

Li, Wei, et al. "Comparative Genomic Sequence Analysis of Chinese Orf Virus Strain NA1/11 With Other Parapoxviruses." Archives of Virology, vol. 160, no. 1, 2015, pp. 253-66.
Li W, Hao W, Peng Y, et al. Comparative genomic sequence analysis of Chinese orf virus strain NA1/11 with other parapoxviruses. Arch Virol. 2015;160(1):253-66.
Li, W., Hao, W., Peng, Y., Duan, C., Tong, C., Song, D., ... Luo, S. (2015). Comparative genomic sequence analysis of Chinese orf virus strain NA1/11 with other parapoxviruses. Archives of Virology, 160(1), pp. 253-66. doi:10.1007/s00705-014-2274-1.
Li W, et al. Comparative Genomic Sequence Analysis of Chinese Orf Virus Strain NA1/11 With Other Parapoxviruses. Arch Virol. 2015;160(1):253-66. PubMed PMID: 25385177.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative genomic sequence analysis of Chinese orf virus strain NA1/11 with other parapoxviruses. AU - Li,Wei, AU - Hao,Wenbo, AU - Peng,Yongzheng, AU - Duan,Chaohui, AU - Tong,Chengbi, AU - Song,Deguang, AU - Gao,Feng, AU - Li,Ming, AU - Rock,Daniel L, AU - Luo,Shuhong, Y1 - 2014/11/11/ PY - 2014/06/16/received PY - 2014/10/30/accepted PY - 2014/11/12/entrez PY - 2014/11/12/pubmed PY - 2015/3/12/medline SP - 253 EP - 66 JF - Archives of virology JO - Arch. Virol. VL - 160 IS - 1 N2 - Orf virus (ORFV) is a typical member of the genus Parapoxvirus. The parapoxvirus genome consists of highly variable terminal regions and relatively conserved central regions with a high G + C content. In our previous study, a novel ORFV strain, NA1/11, was isolated from northeastern China. To fully characterize this strain, we sequenced the entire genome of NA1/11 and conducted a comparative analysis using multiple parapoxviruses. The genomic sequence of NA1/11 was found to consist of 137,080 nucleotides with a G + C content of 63.6 %, but it did not contain the terminal hairpin sequence. Alignment of ORFs from NA1/11 with NZ2, IA82 and SA00 revealed several highly variable ORFs, while the most evident ones are ORFs 001, 103, 109-110, 116 and 132. An odd phenomenon in the region of ORFs 118-120 is that the non-coding fragments are almost as long as the coding fragments. By comparative analysis of inverted terminal repeats, we identified one repeat motif and a long conserved fragment. By comparing the ITRs of SA00 with those of three other ORFVs, more clues were obtained about the correlation between ITR sequence and host adaption. Comparison of the NA1/11 genome with the sequences of other strains of ORFV revealed highly variable regions, thus providing new insights into the genetic diversity of ORFV. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/25385177/Comparative_genomic_sequence_analysis_of_Chinese_orf_virus_strain_NA1/11_with_other_parapoxviruses_ L2 - https://dx.doi.org/10.1007/s00705-014-2274-1 DB - PRIME DP - Unbound Medicine ER -