Tags

Type your tag names separated by a space and hit enter

Epstein-Barr virus-specific intrathecal oligoclonal IgG production in relapsing-remitting multiple sclerosis is limited to a subset of patients and is composed of low-affinity antibodies.
J Neuroinflammation. 2014 Nov 13; 11:188.JN

Abstract

BACKGROUND

The purpose of this study was to investigate intrathecal production and affinity distributions of Epstein-Barr virus (EBV)-specific antibodies in multiple sclerosis (MS) and controls.

METHODS

Cerebrospinal fluid (CSF) and serum concentrations, quantitative intrathecal synthesis, oligoclonal bands (OCB) patterns and affinity distributions of anti-Epstein Barr virus (EBV) antibodies were evaluated in 100 relapsing-remitting MS (RRMS) patients and 200 age- and sex-matched controls with other inflammatory neurological disorders (OIND) and other noninflammatory neurological disorders (NIND).

RESULTS

Levels of anti-EBNA-1 and anti-viral capsid antigen (VCA) IgG were different in both the CSF (P <0.0001 and P <0.01, respectively) and serum (P <0.001 and P <0.05, respectively) among the RRMS, OIND and NIND. An intrathecal synthesis of anti-EBNA-1 IgG and anti-VCA IgG, as indicated by the antibody index, was underrepresented in the RRMS, OIND and NIND (range 1 to 7%). EBV-specific OCB were detected in 24% of the RRMS patients and absent in the controls. High-affinity antibodies were more elevated in the RRMS and in the OIND than in the NIND for CSF anti-EBNA-1 IgG (P <0.0001) and anti-VCA IgG (P <0.0001). After treatment with increasing concentrations of sodium thiocyanate, the EBV-specific IgG OCB had low affinity in all 24 RRMS patients analyzed.

CONCLUSIONS

Our findings do not support the potential role of an EBV persistent brain chronic infection in MS and suggest that an EBV-specific intrathecal oligoclonal IgG production can occur in a subset of MS patients as part of humoral polyreactivity driven by chronic brain inflammation.

Authors+Show Affiliations

Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. massimiliano.castellazzi@unife.it.Department of Medical Sciences, University of Ferrara, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. carlo.contini@unife.it.Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. carmine.tamborino@unife.it.Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. ffasolo@sissa.it.Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. gloria.roversi1981@gmail.com.Department of Medical Sciences, University of Ferrara, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. srcslv@unife.it.Department of Medical Sciences, University of Ferrara, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. roberta.rizzo@unife.it.Department of Neurosciences and Rehabilitation, Azienda Ospedaliero-Universitaria, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. e.baldi@ospfe.it.Department of Neurosciences and Rehabilitation, Azienda Ospedaliero-Universitaria, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. mr.tola@ospfe.it.Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. tiziana.bellini@unife.it.Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. enrico.granieri@unife.it.Department of Neurosciences and Rehabilitation, Azienda Ospedaliero-Universitaria, Via Aldo Moro 8, I-44124, Cona Ferrara, Italy. e.fainardi@ospfe.it.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25391491

Citation

Castellazzi, Massimiliano, et al. "Epstein-Barr Virus-specific Intrathecal Oligoclonal IgG Production in Relapsing-remitting Multiple Sclerosis Is Limited to a Subset of Patients and Is Composed of Low-affinity Antibodies." Journal of Neuroinflammation, vol. 11, 2014, p. 188.
Castellazzi M, Contini C, Tamborino C, et al. Epstein-Barr virus-specific intrathecal oligoclonal IgG production in relapsing-remitting multiple sclerosis is limited to a subset of patients and is composed of low-affinity antibodies. J Neuroinflammation. 2014;11:188.
Castellazzi, M., Contini, C., Tamborino, C., Fasolo, F., Roversi, G., Seraceni, S., Rizzo, R., Baldi, E., Tola, M. R., Bellini, T., Granieri, E., & Fainardi, E. (2014). Epstein-Barr virus-specific intrathecal oligoclonal IgG production in relapsing-remitting multiple sclerosis is limited to a subset of patients and is composed of low-affinity antibodies. Journal of Neuroinflammation, 11, 188. https://doi.org/10.1186/s12974-014-0188-1
Castellazzi M, et al. Epstein-Barr Virus-specific Intrathecal Oligoclonal IgG Production in Relapsing-remitting Multiple Sclerosis Is Limited to a Subset of Patients and Is Composed of Low-affinity Antibodies. J Neuroinflammation. 2014 Nov 13;11:188. PubMed PMID: 25391491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr virus-specific intrathecal oligoclonal IgG production in relapsing-remitting multiple sclerosis is limited to a subset of patients and is composed of low-affinity antibodies. AU - Castellazzi,Massimiliano, AU - Contini,Carlo, AU - Tamborino,Carmine, AU - Fasolo,Francesca, AU - Roversi,Gloria, AU - Seraceni,Silva, AU - Rizzo,Roberta, AU - Baldi,Eleonora, AU - Tola,Maria Rosaria, AU - Bellini,Tiziana, AU - Granieri,Enrico, AU - Fainardi,Enrico, Y1 - 2014/11/13/ PY - 2014/07/28/received PY - 2014/10/16/accepted PY - 2014/11/14/entrez PY - 2014/11/14/pubmed PY - 2015/12/15/medline SP - 188 EP - 188 JF - Journal of neuroinflammation JO - J Neuroinflammation VL - 11 N2 - BACKGROUND: The purpose of this study was to investigate intrathecal production and affinity distributions of Epstein-Barr virus (EBV)-specific antibodies in multiple sclerosis (MS) and controls. METHODS: Cerebrospinal fluid (CSF) and serum concentrations, quantitative intrathecal synthesis, oligoclonal bands (OCB) patterns and affinity distributions of anti-Epstein Barr virus (EBV) antibodies were evaluated in 100 relapsing-remitting MS (RRMS) patients and 200 age- and sex-matched controls with other inflammatory neurological disorders (OIND) and other noninflammatory neurological disorders (NIND). RESULTS: Levels of anti-EBNA-1 and anti-viral capsid antigen (VCA) IgG were different in both the CSF (P <0.0001 and P <0.01, respectively) and serum (P <0.001 and P <0.05, respectively) among the RRMS, OIND and NIND. An intrathecal synthesis of anti-EBNA-1 IgG and anti-VCA IgG, as indicated by the antibody index, was underrepresented in the RRMS, OIND and NIND (range 1 to 7%). EBV-specific OCB were detected in 24% of the RRMS patients and absent in the controls. High-affinity antibodies were more elevated in the RRMS and in the OIND than in the NIND for CSF anti-EBNA-1 IgG (P <0.0001) and anti-VCA IgG (P <0.0001). After treatment with increasing concentrations of sodium thiocyanate, the EBV-specific IgG OCB had low affinity in all 24 RRMS patients analyzed. CONCLUSIONS: Our findings do not support the potential role of an EBV persistent brain chronic infection in MS and suggest that an EBV-specific intrathecal oligoclonal IgG production can occur in a subset of MS patients as part of humoral polyreactivity driven by chronic brain inflammation. SN - 1742-2094 UR - https://www.unboundmedicine.com/medline/citation/25391491/Epstein_Barr_virus_specific_intrathecal_oligoclonal_IgG_production_in_relapsing_remitting_multiple_sclerosis_is_limited_to_a_subset_of_patients_and_is_composed_of_low_affinity_antibodies_ L2 - https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-014-0188-1 DB - PRIME DP - Unbound Medicine ER -