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Protective effect of carboxymethylated chitosan on hydrogen peroxide-induced apoptosis in nucleus pulposus cells.
Mol Med Rep. 2015 Mar; 11(3):1629-38.MM

Abstract

Although the etiology of intervertebral disc degeneration is poorly understood, one approach to prevent this process may be to inhibit apoptosis. In the current study, the anti‑apoptotic effects of carboxymethylated chitosan (CMCS) in nucleus pulposus (NP) cells were investigated with the aim to enhance disc cell survival. Rat NP cells were isolated and cultured in vitro, and hydrogen peroxide (H2O2) was used to build the NP cell apoptosis model. Cell viability was assessed with a cell counting kit‑8 assay. The ratio of apoptotic cells was surveyed by annexin V‑fluorescein isothiocyanate (FITC) and propidium iodide (PI) double staining analysis, and the morphology was observed by Hoechst 33342 staining. The mitochondrial membrane potential of NP cells was evaluated by rhodamine 123 fluorescence staining. Reverse transcription (RT)‑quantitative polymerase chain reaction (qPCR) was performed to measure mRNA levels of inducible nitric oxide synthase (iNOS), caspase‑3, B‑cell lymphoma (Bcl)‑2, type II collagen and aggrecan. Western blot analysis was performed to detect protein levels of iNOS and Bcl‑2. The annexin V‑FITC/PI and Hoechst 33342 staining results indicated that CMCS was able to prevent NP cells from apoptosis in a dose‑dependent manner. Rhodamine 123 staining clarified that CMCS reduced the impairment of the mitochondrial membrane potential in H2O2‑treated NP cells. Reduced caspase‑3 and increased Bcl‑2 activity were detected in CMCS‑treated NP cells by RT‑qPCR and western blot analysis. CMCS also promoted the proliferation and secretion of type II collagen and aggrecan in H2O2‑treated NP cells. CMCS was indicated to be effective in preventing apoptotic cell death in vitro, demonstrating the potential advantages of this therapeutic approach in regulating disc degeneration.

Authors+Show Affiliations

Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25394560

Citation

He, Bin, et al. "Protective Effect of Carboxymethylated Chitosan On Hydrogen Peroxide-induced Apoptosis in Nucleus Pulposus Cells." Molecular Medicine Reports, vol. 11, no. 3, 2015, pp. 1629-38.
He B, Tao H, Liu S, et al. Protective effect of carboxymethylated chitosan on hydrogen peroxide-induced apoptosis in nucleus pulposus cells. Mol Med Rep. 2015;11(3):1629-38.
He, B., Tao, H., Liu, S., & Wei, A. (2015). Protective effect of carboxymethylated chitosan on hydrogen peroxide-induced apoptosis in nucleus pulposus cells. Molecular Medicine Reports, 11(3), 1629-38. https://doi.org/10.3892/mmr.2014.2942
He B, et al. Protective Effect of Carboxymethylated Chitosan On Hydrogen Peroxide-induced Apoptosis in Nucleus Pulposus Cells. Mol Med Rep. 2015;11(3):1629-38. PubMed PMID: 25394560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of carboxymethylated chitosan on hydrogen peroxide-induced apoptosis in nucleus pulposus cells. AU - He,Bin, AU - Tao,Haiying, AU - Liu,Shiqing, AU - Wei,Ailin, Y1 - 2014/11/13/ PY - 2014/02/01/received PY - 2014/09/29/accepted PY - 2014/11/15/entrez PY - 2014/11/15/pubmed PY - 2015/10/10/medline SP - 1629 EP - 38 JF - Molecular medicine reports JO - Mol Med Rep VL - 11 IS - 3 N2 - Although the etiology of intervertebral disc degeneration is poorly understood, one approach to prevent this process may be to inhibit apoptosis. In the current study, the anti‑apoptotic effects of carboxymethylated chitosan (CMCS) in nucleus pulposus (NP) cells were investigated with the aim to enhance disc cell survival. Rat NP cells were isolated and cultured in vitro, and hydrogen peroxide (H2O2) was used to build the NP cell apoptosis model. Cell viability was assessed with a cell counting kit‑8 assay. The ratio of apoptotic cells was surveyed by annexin V‑fluorescein isothiocyanate (FITC) and propidium iodide (PI) double staining analysis, and the morphology was observed by Hoechst 33342 staining. The mitochondrial membrane potential of NP cells was evaluated by rhodamine 123 fluorescence staining. Reverse transcription (RT)‑quantitative polymerase chain reaction (qPCR) was performed to measure mRNA levels of inducible nitric oxide synthase (iNOS), caspase‑3, B‑cell lymphoma (Bcl)‑2, type II collagen and aggrecan. Western blot analysis was performed to detect protein levels of iNOS and Bcl‑2. The annexin V‑FITC/PI and Hoechst 33342 staining results indicated that CMCS was able to prevent NP cells from apoptosis in a dose‑dependent manner. Rhodamine 123 staining clarified that CMCS reduced the impairment of the mitochondrial membrane potential in H2O2‑treated NP cells. Reduced caspase‑3 and increased Bcl‑2 activity were detected in CMCS‑treated NP cells by RT‑qPCR and western blot analysis. CMCS also promoted the proliferation and secretion of type II collagen and aggrecan in H2O2‑treated NP cells. CMCS was indicated to be effective in preventing apoptotic cell death in vitro, demonstrating the potential advantages of this therapeutic approach in regulating disc degeneration. SN - 1791-3004 UR - https://www.unboundmedicine.com/medline/citation/25394560/Protective_effect_of_carboxymethylated_chitosan_on_hydrogen_peroxide_induced_apoptosis_in_nucleus_pulposus_cells_ L2 - http://www.spandidos-publications.com/mmr/11/3/1629 DB - PRIME DP - Unbound Medicine ER -