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Ampelopsis brevipedunculata extract prevents bone loss by inhibiting osteoclastogenesis in vitro and in vivo.
Molecules. 2014 Nov 12; 19(11):18465-78.M

Abstract

Osteoclasts play a critical role in bone resorbing disorders such as osteoporosis, periodontitis, and rheumatoid arthritis. Therefore, discovery of agents capable of suppressing osteoclast differentiation may aid the development of a therapeutic access for the treatment of pathological bone loss. Ampelopsis brevipedunculata has been used as herbal folk medicine to treat liver diseases and inflammation in Asia. However, its effects on osteoclast differentiation are unknown. We were aimed to investigate the anti-osteoclastogenic activity in vitro and in vivo and to elucidate the underlying mechanism of Ampelopsis brevipedunculata extract (ABE). In this study, ABE inhibited receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation, the formation of filamentous actin rings and the bone resorbing activity of mature osteoclasts. ABE inhibited RANKL-induced p38 and IκB phosphorylation and IκB degradation. Also, ABE suppressed the mRNA and protein expression of nuclear factor of activated T cells c1 (NFATc1) and c-Fos, and the mRNA expression of genes required for cell fusion and bone resorption, such as osteoclast-associated receptor (OSCAR), tartrate resistant acid phosphatase (TRAP), cathepsin K, dendritic cell-specific transmembrane protein (DC-STAMP), β3-integrin and osteoclast stimulatory transmembrane protein (OC-STAMP). Furthermore, results of micro-CT and histologic analysis indicated that ABE remarkably prevented lipopolysaccharide (LPS)-induced bone erosion. These results demonstrate that ABE prevents LPS-induced bone erosion through inhibition of osteoclast differentiation and function, suggesting the promise of ABE as a potential cure for various osteoclast-associated bone diseases.

Authors+Show Affiliations

Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. kimjy1014@gmail.com.Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. beryls@wku.ac.kr.Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Jeonbuk 580-185, Korea. ohhm@kribb.re.kr.Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc., Seoul 130-831, Korea. Ksc960@naver.com.Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. phone8418@hanmail.net.Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. ckhlms@wku.ac.kr.Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Jeonbuk 580-185, Korea. rho-m@kribb.re.kr.Imaging Science-based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. jmoh@wku.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25397737

Citation

Kim, Ju-Young, et al. "Ampelopsis Brevipedunculata Extract Prevents Bone Loss By Inhibiting Osteoclastogenesis in Vitro and in Vivo." Molecules (Basel, Switzerland), vol. 19, no. 11, 2014, pp. 18465-78.
Kim JY, Park SH, Oh HM, et al. Ampelopsis brevipedunculata extract prevents bone loss by inhibiting osteoclastogenesis in vitro and in vivo. Molecules. 2014;19(11):18465-78.
Kim, J. Y., Park, S. H., Oh, H. M., Kwak, S. C., Baek, J. M., Lee, M. S., Rho, M. C., & Oh, J. (2014). Ampelopsis brevipedunculata extract prevents bone loss by inhibiting osteoclastogenesis in vitro and in vivo. Molecules (Basel, Switzerland), 19(11), 18465-78. https://doi.org/10.3390/molecules191118465
Kim JY, et al. Ampelopsis Brevipedunculata Extract Prevents Bone Loss By Inhibiting Osteoclastogenesis in Vitro and in Vivo. Molecules. 2014 Nov 12;19(11):18465-78. PubMed PMID: 25397737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ampelopsis brevipedunculata extract prevents bone loss by inhibiting osteoclastogenesis in vitro and in vivo. AU - Kim,Ju-Young, AU - Park,Sun-Hyang, AU - Oh,Hyun Mee, AU - Kwak,Sung Chul, AU - Baek,Jong Min, AU - Lee,Myeung Su, AU - Rho,Mun Chual, AU - Oh,Jaemin, Y1 - 2014/11/12/ PY - 2014/09/30/received PY - 2014/10/28/revised PY - 2014/11/10/accepted PY - 2014/11/15/entrez PY - 2014/11/15/pubmed PY - 2015/10/6/medline SP - 18465 EP - 78 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 19 IS - 11 N2 - Osteoclasts play a critical role in bone resorbing disorders such as osteoporosis, periodontitis, and rheumatoid arthritis. Therefore, discovery of agents capable of suppressing osteoclast differentiation may aid the development of a therapeutic access for the treatment of pathological bone loss. Ampelopsis brevipedunculata has been used as herbal folk medicine to treat liver diseases and inflammation in Asia. However, its effects on osteoclast differentiation are unknown. We were aimed to investigate the anti-osteoclastogenic activity in vitro and in vivo and to elucidate the underlying mechanism of Ampelopsis brevipedunculata extract (ABE). In this study, ABE inhibited receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation, the formation of filamentous actin rings and the bone resorbing activity of mature osteoclasts. ABE inhibited RANKL-induced p38 and IκB phosphorylation and IκB degradation. Also, ABE suppressed the mRNA and protein expression of nuclear factor of activated T cells c1 (NFATc1) and c-Fos, and the mRNA expression of genes required for cell fusion and bone resorption, such as osteoclast-associated receptor (OSCAR), tartrate resistant acid phosphatase (TRAP), cathepsin K, dendritic cell-specific transmembrane protein (DC-STAMP), β3-integrin and osteoclast stimulatory transmembrane protein (OC-STAMP). Furthermore, results of micro-CT and histologic analysis indicated that ABE remarkably prevented lipopolysaccharide (LPS)-induced bone erosion. These results demonstrate that ABE prevents LPS-induced bone erosion through inhibition of osteoclast differentiation and function, suggesting the promise of ABE as a potential cure for various osteoclast-associated bone diseases. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/25397737/Ampelopsis_brevipedunculata_extract_prevents_bone_loss_by_inhibiting_osteoclastogenesis_in_vitro_and_in_vivo_ L2 - https://www.mdpi.com/resolver?pii=molecules191118465 DB - PRIME DP - Unbound Medicine ER -