Tags

Type your tag names separated by a space and hit enter

Determination of delta 1-tetrahydrocannabinol in human fat biopsies from marihuana users by gas chromatography-mass spectrometry.
Biomed Chromatogr. 1989 Jan; 3(1):35-8.BC

Abstract

A gas chromatographic-mass spectrometric (GC/MS) method for analysis of delta 1-tetrahydrocannabinol (delta 1-THC) in human fat samples is described. The fat sample, obtained from heavy marihuana users 1 week before and 4 weeks after smoking, is homogenized in hexane + 2-propanol, centrifuged, and the supernatant mixed with Lipidex 5000. The solvent is evaporated and the dried gel is packed in a glass column. delta 1-THC is eluted from the column with methanol + water + acetic acid, diluted with water and the eluent is passed through a bed of Octadecylsilane-bonded silica. After washing and drying, the retained delta 1-THC is eluted with hexane, derivatized with N-methyl-N-(t-butyl-dimethysilyl)trifluoroacetamide (MTBSTFA) and finally purified by HPLC on an Octadecyl Sl 100 column in methanol. The amount of delta 1-THC is determined by GC/MS, using selected ion monitoring, and a deuterated internal standard. The recovery of delta 1-THC is about 80%, and the concentration of delta 1-THC in the fat samples analysed ranged between 0.4 and 193 ng/g wet tissue.

Authors+Show Affiliations

Department of Pharmacognosy, Uppsala University, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2539872

Citation

Johansson, E, et al. "Determination of Delta 1-tetrahydrocannabinol in Human Fat Biopsies From Marihuana Users By Gas Chromatography-mass Spectrometry." Biomedical Chromatography : BMC, vol. 3, no. 1, 1989, pp. 35-8.
Johansson E, Norén K, Sjövall J, et al. Determination of delta 1-tetrahydrocannabinol in human fat biopsies from marihuana users by gas chromatography-mass spectrometry. Biomed Chromatogr. 1989;3(1):35-8.
Johansson, E., Norén, K., Sjövall, J., & Halldin, M. M. (1989). Determination of delta 1-tetrahydrocannabinol in human fat biopsies from marihuana users by gas chromatography-mass spectrometry. Biomedical Chromatography : BMC, 3(1), 35-8.
Johansson E, et al. Determination of Delta 1-tetrahydrocannabinol in Human Fat Biopsies From Marihuana Users By Gas Chromatography-mass Spectrometry. Biomed Chromatogr. 1989;3(1):35-8. PubMed PMID: 2539872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of delta 1-tetrahydrocannabinol in human fat biopsies from marihuana users by gas chromatography-mass spectrometry. AU - Johansson,E, AU - Norén,K, AU - Sjövall,J, AU - Halldin,M M, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 35 EP - 8 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 3 IS - 1 N2 - A gas chromatographic-mass spectrometric (GC/MS) method for analysis of delta 1-tetrahydrocannabinol (delta 1-THC) in human fat samples is described. The fat sample, obtained from heavy marihuana users 1 week before and 4 weeks after smoking, is homogenized in hexane + 2-propanol, centrifuged, and the supernatant mixed with Lipidex 5000. The solvent is evaporated and the dried gel is packed in a glass column. delta 1-THC is eluted from the column with methanol + water + acetic acid, diluted with water and the eluent is passed through a bed of Octadecylsilane-bonded silica. After washing and drying, the retained delta 1-THC is eluted with hexane, derivatized with N-methyl-N-(t-butyl-dimethysilyl)trifluoroacetamide (MTBSTFA) and finally purified by HPLC on an Octadecyl Sl 100 column in methanol. The amount of delta 1-THC is determined by GC/MS, using selected ion monitoring, and a deuterated internal standard. The recovery of delta 1-THC is about 80%, and the concentration of delta 1-THC in the fat samples analysed ranged between 0.4 and 193 ng/g wet tissue. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/2539872/Determination_of_delta_1_tetrahydrocannabinol_in_human_fat_biopsies_from_marihuana_users_by_gas_chromatography_mass_spectrometry_ L2 - https://doi.org/10.1002/bmc.1130030109 DB - PRIME DP - Unbound Medicine ER -